...flowers inside. 50 mL falcon tubes or plastic weigh boats are both great options for this, depending on the...

...becomes available. For routine infections, like strep throat, testing for antibiotic resistance isn’t commonly...

...through microscopes, and sometimes you even wear a lab coat! These are the things the rest of the world imagines...

...are several variants of the CRISPR/Cas9 system floating around, and many pre-designed plasmids containing...

...primers. These are then immobilized with streptavidin coated beads. Fluorescently labelled dCas9 complexes containing...

...proteins that are tagged with biotin using avidin coated purification techniques. Due to the high affinity...

...easily purified using columns or beads that are coated with avidin. The biotin-avidin interaction has...

...). RNA production can be detected by using a MS2 coat protein that specifically detects a specific RNA...

... too easy to see ‘real’ science as wearing a lab coat and working at the bench, but the reality is a little...

... like Albert Einstein and Bill Nye in their lab coats handling glassware with brightly colored hazardous...

...out, marched with Truth. She was a woman in a lab coat.  The march opened with speeches but it is how the...

...antibodies are derived from animals, such as rabbits or goats; the animals are injected with the antigen that ...

...primary conjugated antibody. Overview of application Coat antigens to the well of a plate, either by charge...] [primary source secondary recognizes], e.g. , “goat anti-rabbit.” Choose a secondary that recognizes...

... RNA (gRNA), while the CRY2PHR-activator fusion floats freely, depicted in the figure below. Once triggered...

...differences in viral uptake by cells and to the uncoating step in the viral life cycle. Using AAV for neuronal...

...prior to handling the materials. Equipment Weighing boats or weighing paper Balance Scoopula or spatula Container...the reagent you will be weighing out, a weighing boat or weighing paper to contain the reagent while on...larger than the capacity of your balance or weighing boat, you can weigh out the reagents in batches. For ...your container after each time. Place the weighing boat or weighing paper onto the balance. These are used...paper could stick to the surface. Tare the weighing boat or paper. You need to do this because you don’t ...t want to include the weight of the weighing boat or paper into your measurement. Hit the tare button ... the material you are weighing onto the weighing boat or paper. If you weighed out too much material, ...

... Many items may float in the water bath when they are placed inside. Water bath floats can be used to ...Disinfectant Thermometer Water bath weights and floats Reagents None needed Procedure Ensure that the ...on the side of the tube. This way, as items are floating, you do not need to necessarily maneuver the bottles...

... for tips on using PPE in the lab. Equipment Lab coat Close-toed shoes Gloves Eye protection and face ...face shields, as needed Guidelines Your lab coat should be buttoned to protect your body and clothing from... gloves in a biohazard waste container. Your lab coat and gloves should not be worn in "common areas" ...

...requires laboratory glasses in addition to coat and gloves. This lab coat should not be worn outside of the BSL...learn more about safety in the lab. Equipment Lab coat Close-toed shoes Gloves Eye protection or face shields...

...in the vapors. Workflow Timeline Day 1: Antigen Coating Day 2: Blocking Day 3: Primary antibody incubation...Procedure Section 1: Prepare the Antigen Standard and coat the plate Dilute purified antigen to 20 ng/µL in...concentration!). Sample Data Figure 1: The plate was coated with serial dilutions of human recombinant purified...