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We narrowed to 364 results for: Nov

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Showing: 41 - 60 of 364 results

  1. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...Originally published Nov 12, 2015 and last updated Aug 20, 2020. Cas9 can be used to modify any desired...ways to circumvent this limitation: 1) the use of novel S. pyogenes Cas9 variants with varying PAM sequences... from IGI!) Synthetic S. pyogenes Cas9s with novel PAM recognition In 2015, Keith Joung’s lab performed...2015). From these screens, they identified four novel SpCas9 variants with altered PAM binding specificity...remaining variants (VQR, EQR, and VRER) recognize novel PAM sequences (shown above). The VQR, EQR, and VRER... Expanding the CRISPR toolbox The isolation of novel CRISPR proteins has and will continue to dramatically...

  6. Gibson Assembly Protocol

    Type
    Protocol
    ...colleagues at the J. Craig Venter Institute developed a novel method for the easy assembly of multiple linear ...

  7. General Transfection

    Type
    Protocol
    ...limited effect on cell growth. Last reviewed on: November 7, 2023...

  9. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Determination of Lentiviral Infectious Titer by a Novel Droplet Digital PCR Method. Hum Gene Ther Methods...

  10. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ... copGFP from the PGK promoter Adenoviral Transfer Vectors The adenoviral transfer vectors contained within...on lentiviral and adenoviral vectors read our Lentiviral Vector Guide and Adenoviral Vector Guide Find...Core Viral Vectors Lentiviral Transfer Vectors Adenoviral Transfer Vectors Resources Kazuhiro Oka and the...Medicine have deposited a series of lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer...pAdenoX from Clontech and can be used to generate adenovirus expressing a variety of reporters as well as ...process as it can be used in blue–white screening . Adenoviral Vector ID Purpose pAdx-CMV-LacZ 73345 Expresses... more plasmids in our Lentiviral Plasmids and Adenoviral Plasmids collections Addgene Blog: Your Lentiviral...

  11. TALEN Guide

    Type
    Collection
    ...Martin-Luther-University Halle-Wittenberg and Adam Bogdanove at Iowa State University published the nucleotide...zinc finger community ( see interview with Adam Bogdanove ), progress on TALe research has been swift. The...Figure 2: Simplified representation of the Voytas/Bogdanove Golden Gate TALEN kit. In this example, the final...technically challenging. Thanks to efforts by the Bogdanove group and Daniel Voytas’s group from the University...backbones in just a few steps ( Figure 2 ). The Bogdanove group also hosts web-based software to help scientists...hundreds of unique visitors every month. The Voytas/Bogdanove TALEN kit came online in the summer of 2011 and...Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Jul;39(...

  12. COVID-19 Resources

    Type
    Collection
    ... Resources Image credit: Maya Kostman for the Innovative Genomics Institute The Coronavirus Disease-2019...2019 (COVID-19) pandemic was caused by a novel virus strain from the Coronaviridae family called SARS-...speed to elucidate as much as they could about this novel virus. SARS-CoV-2 entry into a host...in a new window) For rapid detection of the 2019 novel coronavirus SARS-CoV-2 using CRISPR diagnostics:...bioRxiv (Link opens in a new window) Elsevier's Novel Coronavirus Information Center (Link opens in a ... a new window) Wiley Online Library - Covid-19: Novel Coronavirus Content Free to Access (Link opens in...., Chen, Z. N. (2020). CD147-spike protein is a novel route for SARS-CoV-2 infection to host cells . Signal...

  13. Immunology Research Plasmids and Resources

    Type
    Collection
    ...urokinase receptor CD87, UPAR, URKR PLXNA1 plexin A1 NOV, NOVP, PLEXIN-A1, PLXN1 PLXNA2 plexin A2 FLJ11751, FLJ30634...receptor - NODAL nodal homolog (mouse) MGC138230 NOV nephroblastoma overexpressed gene CCN3, IGFBP9 NPFF... in early stage tumors-like 1 cdnf ARTN artemin ENOVIN, EVN, NBN AVP arginine vasopressin ADH, ARVP, AVP-NPII...tissue growth factor CCN2, HCS24, IGFBP8, MGC102839, NOV2 CTSG cathepsin G CG, MGC23078 CX3CL1 chemokine (...

  14. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ... vector serotype. A helper plasmid containing adenoviral or herpesviral helper genes. These genes are ...serotype. Some packaging plasmids also include adenoviral helper genes normally contained in the helper...packaging in specific serotypes. Some also include adenoviral helper genes (E4, E2a, and VA), eliminating the...require the presence of a "helper" virus, such as adenovirus or herpes simplex virus, to replicate in the ...cell lines. Helper plasmids generally encode the adenoviral E4, E2a, and VA genes. This table contains helper...

  15. Viral Vector Guides and Plasmids

    Type
    Collection
    ...lines or clinical use. Adenoviral Vector Guide | Adenoviral Plasmids Adenoviral vectors have high packaging...kb 18–26 nm Dividing/Non-dividing No* Very Low Adenoviral Transient Double-stranded linear DNA 5–36 kb ...

  16. CRISPR Guide

    Type
    Collection
    ...Seegers, C. L., Bollen, S., Jore, M. M., Semenova, E., Severinov, K., De Vos, W. M., Dame, R. T., De Vries...control both when and where genome editing happens. CASANOVA , developed by Dominik Niopek’s lab , is a way...It has also been used extensively to screen for novel genes that regulate known phenotypes, including ... maintained by the Jennifer Doudna lab at the Innovative Genomics Institute, University of California,... 35301321 Edraki, A., Mir, A., Ibraheim, R., Gainetdinov, I., Yoon, Y., Song, C., Cao, Y., Gallant, J....30403660 Sanson, K. R., Hanna, R. E., Hegde, M., Donovan, K. F., Strand, C., Sullender, M. E., Vaimberg,..., Gootenberg, J. S., Wu, W. Y., Scott, D. A., Severinov, K., Van Der Oost, J., & Zhang, F. (2017). Multiplex...

  17. Luciferase Plasmid Collection

    Type
    Collection
    ... of imperfect miRNA sequence in the 3' UTR Carl Novina 33154 pAC-Luc Firefly Creation of N-terminal Firefly... Return to Top Other Highlighted Tools Modified/Novel Luciferases Engineered variants of luciferases with...and neoLux1.2 : Artificial luciferases desined de novo using deep-learning approaches. Split Luciferase...Renilla luciferase and a Halo tag to assay mRNA turnover in real time. Nonsense-mediated decay reporter...

  18. Resolute Plasmid Collection

    Type
    Collection
    ...members from industry and academia financed by the Innovative Medicine Initiative (IMI) of the European Union...RESOLUTE project has received funding from the Innovative Medicines Initiative 2 Joint Undertaking under...the European Union's Horizon 2020 research and innovation programme and EFPIA. Figure 1: Representation...

  19. Adenovirus Guide

    Type
    Guide
    ...Guides Viral Vectors Adenoviral Vector Guide Adenoviral Vector Guide Jump To: Adenoviral Vectors Vector Production...the end! Browse Adenoviral Plasmids Adenovirus versus Adeno-Associated Virus Adenoviruses fall under the...recombination with the adenoviral plasmid. Adenoviral plasmid — containing the rest of the adenoviral genome necessary... of all adenoviral plasmid components, see the Adenoviral Plasmid Elements table . Adenoviral Generations...Introduction to Adenovirus Adenoviral Vector Production and Troubleshooting Adenoviral Delivery of CRISPR... No more helper adenovirus: production of gutless adenovirus (GLAd) free of adenovirus and replication-competent... Kosai, K. I. (2021). Adenovirus biology, recombinant adenovirus, and adenovirus usage in gene therapy...

  20. Brain Initiative Collection

    Type
    Collection
    ...Collection The Brain Research through Advancing Innovative Neurotechnologies® (BRAIN) Initiative aims to...brain through the development and application of innovative tools enabling large-scale real-time interrogation...brain generated through this effort will provide novel ways to treat, cure, and even prevent brain disorders...
Showing: 41 - 60 of 364 results