...cameleon 3.60, EPAC, and Galphai), the C-terminus and N-terminus of the donor fluorescent protein can be truncated...increase the FRET efficiency.
Creating fusions at the N- and C- termini of your protein of interest
The most...straightforward fusion protein is made by connecting the N- or C-terminus of the fluorescent protein and protein...to describe the different parts of the fusion from N- to C-terminus. For instance, Lifeact-mTurquoise2 ...We have encountered several proteins for which the N- and C-terminus of the POI are necessary for its native...fluorescent protein into the POI can work well since the N- and C-terminal residues of the fluorescent protein...monomers in mammalian cells include: mTurquoise2, mEGFP, mNeonGreen, mScarlet(-I), and mCherry.
Keep the...