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Showing: 41 - 48 of 48 results
  1. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...Terminus 79629 (N) and 79628 (C) Cv (capsid) N and C 79633 (N) and 79641 (C) NS1 (var: W98G) N and C 79635...NS2B C 79636 NS4A N 79640 NS4B C 79639 NS5 C 79632 (N) and 79631 (C) PrM N and C   Detecting...Hess et al. successfully evolved wild type GFP to EGFP using CRISPR-X and subsequent FACS sorting. They.... The Roux lab has deposited HA tagged BioID2 for N-terminal fusions and Myc tagged BioID2 for C-terminal... (< 100 bp) and large (up to 120 kb) deletions in N. benthamiana and Arabidopsis. The authors found that...
  2. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...cameleon 3.60, EPAC, and Galphai), the C-terminus and N-terminus of the donor fluorescent protein can be truncated...increase the FRET efficiency. Creating fusions at the N- and C- termini of your protein of interest The most...straightforward fusion protein is made by connecting the N- or C-terminus of the fluorescent protein and protein...to describe the different parts of the fusion from N- to C-terminus. For instance, Lifeact-mTurquoise2 ...We have encountered several proteins for which the N- and C-terminus of the POI are necessary for its native...fluorescent protein into the POI can work well since the N- and C-terminal residues of the fluorescent protein...monomers in mammalian cells include: mTurquoise2, mEGFP, mNeonGreen, mScarlet(-I), and mCherry. Keep the...
  3. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...pAdx-CMV-iCre-p2A-copGFP 73350 Expresses iCre and EGFP from the CMV promoter pAdx-CMV-iCre-P2A-tdTomato...Cre is coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase) pCDH-CB-copGFP-T2A-iCre 72256...
  4. CRISPR References and Information

    Type
    Collection
    ...plasmids: pVSVg , psPAX2 ; positive control: CMV-EGFP PDF 2.3 MB Zhang GeCKO pooled library amplification...packaging plasmids: pVSVg , psPAX2 positive control: CMV-EGFP Kits are also available (mouse or human libraries... and can work for S. pyogenes, S. thermophilus or N. meningitidis Cas9 PAMs. Currently supports: mouse...
  5. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ...genomic regions of interest 82613 3xFLAG-dCas9/MSCV-EGFP Expresses 3xFLAG-dCas9 in mammalian cells for enChIP...enChIP analysis. Fujita T, Kitaura F, Oji A, Tanigawa N, Yuno M, Ikawa M, Taniuchi I, Fujii H. Genes Cells...
  6. Luciferase Plasmid Collection

    Type
    Collection
    ... 87067 pcDNA3.1-ccdB-Nanoluc NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko...87078 pLenti6.2-Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning. Lentiviral...UTR Carl Novina 33154 pAC-Luc Firefly Creation of N-terminal Firefly fusions in Drosopholia Michael Rosbash... 87070 pcDNA3.1-Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko...Taipale 87068 pcDNA3.1-Renilla-ccdB Renilla Creation of N-terminal Renilla luciferase fusions using Gateway ...Taipale 87069 pcDNA3.1-Firefly-ccdB Firefly Creation of N-terminal Firefly luciferase fusions using Gateway ... 87077 pLenti6.2-Firefly-ccdB Firefly Creation of N-terminal Firefly luciferase fusions using Gateway ...
  7. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...IgG2a 182104 Anti-LRRK2/Dardarin, N-terminus [N138/6R] LRRK2/Dardarin, N-terminus Human Mouse IgG2a 182105... 135224 GC52 pEGFPN1 anti-Gephyrin nanobody GC52 Gephyrin Mouse Llama 135225 AC50 pEGFPN1 anti-AMIGO-1...Llama 135226 AC27 pEGFPN1 anti-AMIGO-1 nanobody AC27 AMIGO-1 Mouse Llama 135227 IC65 pEGFPN1 anti-IRSp53 nanobody... nanobody IC65 IRSp53 Mouse Llama 135228 SS80 pEGFPN1 anti-SAPAP2 nanobody SS80 SAPAP2 Mouse Llama 145818...
Showing: 41 - 48 of 48 results