...CEPIA (additional versions are available to simultaneously measure calcium changes in the ER, cytosol ...fluorescence to a red fluorescent state only by simultaneous (1) user-controlled illumination with violet...

...D, member 1 CD94 LGMN legumain AEP, LGMN1, PRSC1 LTA lymphotoxin alpha (TNF superfamily, member 1) LT,...sterile alpha motif containing 1 FLJ31641, RIFLE, TAL LTA lymphotoxin alpha (TNF superfamily, member 1) LT,...receptor delta constant - TRDD1 T cell receptor delta diversity 1 - TRDD2 T cell receptor delta diversity...receptor delta diversity 3 TCRD TRDJ1 T cell receptor delta joining 1 TCRD TRDJ2 T cell receptor delta joining...receptor delta joining 3 - TRDJ4 T cell receptor delta joining 4 - TRDV1 T cell receptor delta variable... IL1F5 interleukin 1 family, member 5 (delta) FIL1, FIL1(DELTA), FIL1D, IL1HY1, IL1L1, IL1RP3, MGC29840...

...perform its helicase and nuclease functions simultaneously, by continuously unraveling dsDNA, pulling ...reeling of ssDNA and unwinding of dsDNA occur simultaneously, which creates a loop in the target strand ...

..., unlike voltages, you can adjust compensation values later in your analysis, unlike voltages.  Figure...

...for instance, the challenge of attempting to simultaneously and selectively induce both cutting and regulation... targeting a different set of loci to induce simultaneous cutting and transcriptional activation or repression...

...cytosine (C) into a uracil (U) base. Later the resultant U-G pair is either repaired by cellular mismatch...enables us to target more than one genomic site simultaneously.     These binary vectors could be used to ...

...of its ability to target multiple gene loci simultaneously, CRISPRa can also be used to map molecular ...nmeth.3871 Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning...

...overnight revelation, rather it was the result of consultations between researchers and publishers. Nonetheless...encouraging authors to integrate datasets with their resultant figures. Instead of providing a “dead end” figure...

...channelrhodopsins and tools based on light-oxygen-voltage (LOV) domain. They can also be used in crosstalk-free...directed evolution approach applied to fluorescent voltage reporters. Nat Chem Biol 14, 352-360 (2018). Yu...

...gel purification. In gel purification, you use a voltage difference across a gel matrix (usually agarose...positioned toward the cathode (- charge). When the voltage is applied across the gel, the DNA migrates toward...

... tend to show their limits when it comes to simultaneously modifying multiple loci (multiplexing). Currently...(MAGE) (1). MAGE uses recombineering (2) to simultaneously incorporate multiple single-strand DNA (ssDNA...in a broad range of strains. Thus, pORTMAGE simultaneously allows genome editing and mutant library generation...

... With this technique, multiple genes can be simultaneously silenced if a consensus sequence is used (~... tissue for downstream characterization. To simultaneously address both limitations of the traditional...

...proteins. A type VII is already on the horizon (Altae-Tran et al., 2023)! References and Resources References...), 280–284. https://doi.org/10.1038/nature24049 Altae-Tran, H., Kannan, S., Suberski, A. J., Mears, K....

...production plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression Edward Boyden...pGP-AAV-syn-FLEX-Volton2-ST-WPRE AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression...4AA-mNeon-ST A122D WPRE AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression...floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector...pAAV-EF1a-DIO-JEDI-2P-WPRE Double floxed genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector...2P-Kv-WPRE Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector...AAV9 pAAV-hSyn-JEDI-2P-WPRE Genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector...

...73472 RabV CVS-N2c(deltaG)-mCherry-P2A-Cre Cre CMV RABV Jessell 73474 RabV CVS-N2c(deltaG)-Cre Cre CMV RABV... light-inducible Cre; C terminus Cre fused to PhyBdelta CMV Mammalian, Zebrafish Eisenhoffer 131782 pME-CreLite...pME-CreLite CreLite system components, PhyBdeltaCreC and PIF6CreN, in middle entry vector (Tol2 kit) Zebrafish...131785 pAAV-CreLite CreLite system components, PhyBdeltaCreC and PIF6CreN, in AAV donor/transfer vector Cbh...131786 pLenti-CreLite CreLite system components, PhyBdeltaCreC and PIF6CreN, in lentiviral vector Cbh Lentiviral...to turn off the expression of one gene while simultaneously turning on another. The FLEx switch system ...

...Mouse IgG2a 114515 Anti-GABA(A)R, Delta [N151/3.3R] GABA(A)R, Delta Rat Mouse IgG2a 114516 Anti-Doc2b ...Mouse IgG2a 206657 Anti-GABA(A)R, Delta [N151/3R-2b] GABA(A)R, Delta Rat Mouse IgG2b 206658 Anti-Navbeta4...Mouse IgG2a 219470 Anti-Cavalpha2delta2 Ca2+ channel [N492/38R] Cavalpha2delta2 Ca2+ channel Mouse Mouse...Human Mouse IgG2a 220415 Cavalpha2delta2 Ca2+ channel [N492/39R] Cavalpha2delta2 Ca2+ channel Mouse Mouse...Mouse IgG2a 220416 Cavalpha2delta2 Ca2+ channel [N492/93R] Cavalpha2delta2 Ca2+ channel Mouse Mouse IgG2a...Human Mouse 206724 GABA(A)R, Delta scFv [N151/3] N151/3 scFv GABA(A)R, Delta Rat Mouse 206725 TRIP8b (constant...

...protein that enables imaging of two biosensors simultaneously   Researchers in the labs of Michael Lin ...mCyFP1 and EGFP as FRET donors in experiments simultaneously measuring the activities of CaMKII and RhoA...

...Sensors GRAB_NE nLight Oxytocin (OT) Sensors GRAB_OT Voltage Sensors Archon Voltron JEDI-2P ATP Sensors iATPSnFR2...GRAB_OTmut (control) none Constitutive 1, 9 Li Voltage Reporter: Archon 108422 pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2...KGC-GFP-ER2] Syn Archon1 EGFP Cre dependent 8 Boyden Voltage Reporter: Voltron 119036 pAAV-hsyn-flex-Voltron-ST...ST-WPRE Syn Voltron2-ST none Cre dependent 1 GENIE Voltage Reporter: JEDI-2P 179459 pAAV-EF1a-DIO-JEDI-2P-...

... al. , 2010). Number of fragments assembled simultaneously Multiple fragments can be assembled in one ...

...plasmid ID 42230). This plasmid allows for the simultaneous expression of sgRNA and SpCas9, but does not...complement oligo ( e.g., sgRNA-A in Table 4 ). The resultant oligos would be 25-mer oligos. However, if the...the reverse complement oligo. In this case, the resultant oligos would be 24-mer oligos ( Table 4 ). Design...