We narrowed to 79 results for: KAN
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TypeBlog Post...References 1. Zhou, Xin X., Linlin Z. Fan, Pengpeng Li, Kang Shen, and Michael Z. Lin. "Optical control of cell...
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Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages
TypeBlog Post...densely populated places like the University of Kansas and the University of Louisville in Kentucky, the... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog Post...133–138. PubMed PMID: 20010839. 4. Caussinus, E., Kanca, O., and Affolter, M. “Fluorescent fusion protein... -
Plasmids 101: Golden Gate Cloning
TypeBlog Post...method with high throughput capability. Engler C, Kandzia R, Marillonnet S. PLoS One 2008;3(11):e3647. doi... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post...chloramphenicol, erythromicin, gentamicin, hygromycin, kanamycin, nourseothricin (clonNat), spectinomycin, streptomycin... -
Viral Vectors 101: Calcium Sensors
TypeBlog Post...Neuroscience Research. 2021 Aug 1;169:2-8. Suzuki J, Kanemaru K, Iino M. Genetically encoded fluorescent indicators... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog Post...K, Sanders E, Tasissa M, Kostman M, Tillgren M, Makana Hanley L, Mueller I, Mitsopoulos A, Fan M (2020... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog Post...K, Sanders E, Tasissa M, Kostman M, Tillgren M, Makana Hanley L, Mueller I, Mitsopoulos A, Fan M (2020... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog Post... protein characteristics. For example, Masato Kanemaki's lab has developed a CRISPR/Cas-based system for... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog Post...1, 18-28. PubMed PMID: 22428977. 2. Choi, V.W., Asokan, A., Haberman, R.A., & Samulski, R.J. (2007). Production... -
Plasmids 101: Multicistronic Vectors
TypeBlog Post...Lentiviral AmCyan-P2A-mCherry P2A Mammalian pC5Kan-P2A P2A Insect pUltra P2A and T2A Lentiviral ... -
MXS Chaining
TypeBlog Post...PubMed Central PMCID: PMC2822740. 2. Engler C, Kandzia R, Marillonnet S. A one pot, one step, precision... -
Tips for arabidopsis transformation
TypeBlog Post... plant selection markers (basta, hygromycin, kananamycin, tunicamycin) (Nakagawa et al., 2007). Alternatively... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog Post...majority of plasmids contain either an ampicillin or kanamycin resistance gene. Every step along the way involves... -
CRISPR Plasmids - Tagging
TypeCollection... CRISPR/Cas Toolkit Kanemaki Lab Auxin-Inducible Degron Tagging Masato Kanemaki's lab has developed a ... -
Plasmids 101: Shuttle Vectors
TypeBlog Post...aminoglycosides work on different host species: kanamycin targets the prokaryotic ribosome, while G418 (... -
Hot Plasmids: Fall 2024
TypeBlog Post...tetracycline repressor TetR and resistance selection marker KanR. This strain is then transformed with the sgRNA ... -
Neuronal labeling with Spaghetti Monster
TypeBlog Post...design in public schools and was started in 2005 in Kansas by Bobby Henderson, a physics graduate from Oregon... -
Plasmids 101: E. coli Strains for Protein Expression
TypeBlog Post... General protein expression m15 pREP4*, ** Kanamycin (pREP4) Cis-repression of the E. coli T5 promoter... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog Post..., Rivera RM, Madhavan S, Pan X, Ran FA, Yan WX, Asokan A, Zhang F, Duan D, Gersbach CA. In vivo genome...