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  1. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ... Shah NG, McKenzie A 3rd, Steever AB, Wach A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14(10):943-51....3rd, Demarini DJ, Shah NG, Wach A, Brachat A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14(10):953-61....

  2. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ... Their cooler boxes come with a prepaid return shipping label. To return the box, simply peel your address...details on what they accept and to print a free shipping label. Recycle nitrile gloves Terracycle is a ...

  3. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    ...coronavirus-related deposits. Our valiant lab and shipping staff agreed to come in and start getting plasmid...plasmid deposits into the collection and to start shipping. They created the best safety protocols they could...

  5. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...line, with a row of FedEx envelopes waiting to be shipped out of the Krogan lab at UCSF. Our team members...Foussard, and Dave Gordon) have been quite busy shipping and sending plasmids to all who request them -...

  7. The MTA at Addgene

    Type
    Blog Post
    ...returned the MTA, Addgene can move forward with shipping the material to the requestor.  Why the MTA?  ...certainly frustrating to find out your plasmid hasn’t shipped because a form needs to be signed. But the truth...

  9. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ... despite the evolving export/import rules. Our shipping and lab teams continue to keep up with the demand...have confidence that from Business Development to Shipping we will execute any new projects with our usual...

  10. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ... and reverse reactions, respectively, and then shipped along with their corresponding glycerol plate on...volume and concentration, and not all providers are equipped to perform Sanger sequencing from glycerol stocks...

  11. Plasmids 101: Cre-lox

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    Blog Post
    ...conceptually similar to Cre-lox, with flippase (Flp) and the short flippase recognition target (FRT) site being...

  13. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...all of your plasmid data, we’ll send you prepaid shipping materials (i.e., a deposit kit) with instructions...we need to know your Addgene account username. Shipping address and phone number. Plasmid sequences or...

  14. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...gRNA/Cas9 to target cells. Therefore, you must be equipped and authorized to use lentivirus in your lab. ...

  15. Common Injection Routes in Mice

    Type
    Blog Post
    ...Restraint: scruffing Location: throat Gauge (bulb-tipped feeding tube): 18–20 Volume: up to 10 ml/kg Skill...Beginner Oral gavage Scruffing Throat 18–20 (bulb tipped) up to 10 mL/kg Intermediate Resources and...

  16. DNA Purification Without a Kit

    Type
    Blog Post
    ...and ball bearings. Then a cellulose dipstick is dipped into this mixture to absorb the nucleic acids. ..., purified total nucleic acids are released by dipping the cellulose directly into a PCR buffer, where...

  17. Tips for arabidopsis transformation

    Type
    Blog Post
    ... Growing the transformed agrobacterium culture Dipping your plant’s flowers in the agrobacterium culture...resuspend them in transformation media. If you are dipping your plants, put the culture into a container that...

  18. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ... editing enzyme (“Editase”), ADAR2, into the hippocampus of a Rett Syndrome mouse model using an adeno-associated...RNA in each of three neuronal subtypes of the hippocampus. This technology has formed the basis of the ...

  20. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...performed the following modifications to pDS132 (Philippe et al., 2004): Replaced the sacB cassette with...PMID: 29760772. PubMed Central PMCID: PMC5946454. Philippe, Nadège, et al. "Improvement of pCVD442, a suicide...
Showing: 41 - 60 of 160 results