...and reduce off-target effects of gene therapy, Feng Zhang’s lab has developed a system called DIRECTED (...

...PMC4744125. 3. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide libraries ...different research constellations: Doench/Root (2), Zhang (3), Wu (4), Yusa (5), Wang/Lander/Sabatini (6),...

...Finding nucleic acids with SHERLOCK and DETECTR . Feng Zhang's lab at the Broad Institute has utilized the ...design resources (Link opens in a new window) Find Zhang Lab Cas13a plasmids Additional protocol for using...

...lentiCas9-Blast (Plasmid #52962) was a gift from Feng Zhang and is described in Improved vectors and genome-wide...libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038...

....0172913 Pubmed PMID: 28245276 Liu X, Wang D, Wang H, Feng E, Zhu L, Wang H (2012) Curing of Plasmid pXO1 from....0029875 Pubmed PMID: 22253811 Ni B, Du Z, Guo Z, Zhang Y, Yang R (2008) Curing of four different plasmids...

...depositors David Liu, PhD, Keith Joung, PhD, and Feng Zhang, PhD, is pursuing CRISPR/Cas9 technology for ...

...-012198382-6/50026-1 Wang R, Xiang S, Feng Y, Srinivas S, Zhang Y, Lin M, Wang S (2013) Engineering production...

...through Addgene. For example, you could enter "Feng Zhang (Addgene plasmid #52961)".   The vector type ...

...PMC2629495. Zingg, B., Chou, X., Zhang, Z., Mesik, L., Liang, F., Tao, H.W., and Zhang, L.I. (2017). AAV-mediated... S.A., Slaughterbeck, C.R., Wakeman, W., Li, Y., Feng, D., Ho, A., Nicholas, E., Hirokawa, K.E., Bohn,...

...luciferase is used to monitor viral transfection Feng Zhang 21471 pLenti PGK V5-LUC Neo (w623-2) Firefly ...

...480–489. PMID: 33230293 Yang, S., Zhang, Y., Xu, J., Zhang, J., Zhang, J., Yang, J., Jiang, Y., & Yang,...CRISPR-associated transposases ( CASTs , developed by the Feng Zhang lab ) combine the large-scale capacity of transposases... mammalian cells. SHERLOCK , developed by the Feng Zhang lab , is a Cas13a-based molecular detection platform...its catalytic domain. REPAIR , developed by the Feng Zhang lab , used a hyperactive adenosine deaminase ...eLife . 3 e04766. PMID: 25497837 Ma, Y., Zhang, J., Yin, W., Zhang, Z., Song, Y., & Chang, X. (2016). Targeted... P. D., Wu, X., Jiang, W., Marraffini, L. A., & Zhang, F. (2013). Multiplex genome engineering using CRISPR...Bravo, J. P. K., Hooper, M. M., Dangerfield, T. L., Zhang, H., Finkelstein, I. J., Johnson, K. A., & Taylor...

...Cummins, A., Chen, Y., Fan, Y., Zhang, Q., Sharma, J., Duan, Y., Wang, L., Feng, G., Chen, Y., Ip, N. Y., Pickel....2022.05.003 Chen, X., Wolfe, D. A., Bindu, D. S., Zhang, M., Taskin, N., Goertsen, D., Shay, T. F., Sullivan...Kim, Y., Toong, N., Kaplow, I. M., Wirthlin, M., Zhang, X., Phan, B. N., Fox, G. A., Wade, K., He, J., ...Schneider, G., Stevenson, O., Vergara, J., Sharma, J., Zhang, Q., Franken, T. P., Smith, J., Ibrahim, L. A., ...

...'s Lab: Optogenetics Resource Center Boyden ES, Zhang F, Bamberg E, Nagel G, Deisseroth K. 2005. Millisecond-timescale...Neurosci. 17(8):1123-9. PMID 24997763 Deisseroth K, Feng G, Majewska AK, Miesenböck G, Ting A, Schnitzer ...Brain Res. 196: 1-28. PMID 22341318 Gradinaru V, Zhang F, Ramakrishnan C, Mattis J, Prakash R, Diester .... 1408:141-65. PMID 26965121 Yizhar O, Fenno L, Zhang F, Hegemann P, Diesseroth K. 2011. Microbial opsins...dysfunction. Nature. 477(7363):171-8. PMID 21796121 Zhang F, Prigge M, Beyrière F, Tsunoda SP, Mattis J, Yizhar...

...J, Inoue K, Kumata K, Seki C, Ono M, Shimojo M, Zhang M-R, Tomita Y, Nakahara J, Suhara T, Takada M, Higuchi...Link opens in a new window) Xiong M, Tao Y, Gao Q, Feng B, Yan W, Zhou Y, Kotsonis TA, Yuan T, You Z, Wu... Haberman A, Graham J, Block J, Zhou W, Chen Y, Zhang S-C (2021). Human Stem Cell-Derived Neurons Repair...

...domain and a mostly c-terminal nuclease domain. Feng Zhang's group utilized the bi-lobed architecture of ... of varying fusion protein expression levels. Zhang et al., PLoS One. 2012;7(8):e43072. doi: 10.1371...