...Transfer (BRET) CalFlux VNT : An intracellular calcium sensor created by fusing NanoLuc® and Venus fluorophore...interest can also be inserted into the plasmid. Malcolm Moore 51819 Syn-ATP Firefly (modified) CMV Optical...Lentiviral expression of firefly luciferase Javier Alcudia 113442 pcDNA3.1 NL NanoLuc® CMV Mammalian expression...

...it to create a standard curve on your blot to calculate the amount in your sample. (Do ensure your blot.../Reprobe PVDF membrane Increased time Calculate absolute quantification Running a standard curve...wells; purified protein not always available Calculate relative quantification Normalizing to ubiquitously...

...by the blood stage of the malaria parasite, P. falciparum . Please see the following reference for more...essential for erythrocyte invasion by Plasmodium falciparum. Crosnier C et al., Nature. 2011 Nov 9;480(7378...

... CRISPR-based method for cell lineage tracing Valcyrie is a CRISPR-based marker excision method for marking...target. Read the paper in Development Find the Valcyrie plasmids If you have a new CRISPR tool you’ve...

...15R] QKI-7 Human Mouse IgG2a 177508 Anti-NALCN [N185/7R] NALCN Rat Mouse IgG2a 177509 Anti-Dopamine D2 ...Mouse IgG2b 225412 NALCN (Rattus norvegicus) recombinant monoclonal antibody. NALCN Rat Mouse IgG2b 225413...Alpha1 Human Mouse 206721 NALCN ion channel scFv [N185/7] N185/7 scFv NALCN ion channel Rat Mouse 206722...

... lot of their plastic packaging (from Corning®, Falcon®, or Axygen® product packaging) for free! It has... If you use serological pipettes from Corning®, Falcon®, or Axygen®, set up a bin or box in your tissue...

...by the blood stage of the malaria parasite, P. falciparum . Please see the following reference for more...bio-His 53410 PCYOX1L-bio-His 53411 PF4-bio-His 53412 CALCR-bio-His 53413 PPIB-bio-His 53414 PTGS1-bio-His 53415...

...-encoded reporters of molecular signals; e.g., calcium indicators In this overview, we will focus on the... truncated forms of cytosolic STIM1 to control calcium channels ~488 LOV domains LOV-based Light-oxygen-voltage-sensing...light-controlled molecules. ~450–495 BACCS light-activated calcium channel switch iLID Improved light-inducible dimers...

...manipulation in the lab. This is commonly done using calcium chloride which permeabilizes the cell membrane ...and physical methods in the lab. Chemicals like calcium phosphate and diethylaminoehtyl (DEAE)-dextra neutralize...

...will image these neurons using GCaMP7, measuring calcium signaling related fluorescence during morphine ...enable multiplexed imaging of neurotransmitter, calcium, and membrane voltage,” says Tian. Figure...

...the transfection method (i.e. polyethyleneimine, calcium phosphate or liposomal methods), the volume of ...be optimized prior to starting. We recommend calculating the ratio of the surface area of the larger vessel...

...qPCR Protocol for more details. Pro Tips When calculating the titer for your AAV, remember to account for...tool for gene expression in mammals Use the PEI calculator for planning AAV packaging transfections Find...

... plate Fidelity of assembly (percent correct) Calculated colony totals Per 2 μl assembly reaction Per...outgrowth volumes described in Figure 3, with calculated yields from entire outgrowth built from 2 µl ...

...to your normal control - you can set up your calculations so that your assay output number (RQ value) ... determination of amplification of Plasmodium falciparum multidrug resistance-1 gene (pfmdr1). J Parasitol...

...vectors expressing GCaMP6f with this promoter. Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f...terminal providing better signal to noise for imaging calcium dynamics in fine structures. We have expanded our...

... commonly used are GECIs - genetically encoded calcium indicators (e.g. GCaMP). Others include: pHluorin... of how bright is the emission. Brightness is calculated as the product of extinction coefficient and ...

...-55 °C for the homologous region (calculated using Oligo Calculator). Insertions - To insert short sequences...

... and ways to navigate it, we’ll search for the calcium biosensor, GCaMP, and click through to the plasmid...distribution. If your experiment involves delivering a calcium biosensor using AAV, you might decide you’d really...

...also need to know the extinction coefficient to calculate the concentration. At 280 nm, the extinction coefficient...protein standard and the concentration may be calculated using imaging software. Any bands on the gel ...

... ligands (Figure 2). Biosensors, which include calcium and voltage sensors, are the broadest category ... vectors available in the repository. GCaMP, a calcium indicator (biosensor, by Addgene’s categorization...