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We narrowed to 111 results for: gfp fluorescence reporter

Showing: 81 - 111 of 111 results

  1. Worm Expression Resources

    Type
    Collection
    ... which makes them attractive for imaging and fluorescence microscopy experiments. The C. elegans genome...genome is well studied and hundreds of reporters have been developed and characterized which allow researchers...This includes CRISPR, RNAi, empty backbones, fluorescent proteins, and Cre expression plasmids. Large ...vectors for C. elegans research, including lacZ and GFP fusion vectors. C. elegans optimized fluorophores...Approach to Better CRISPR/Cas9 Editing Efficiency Fluorescent Tagging of Endogenous Genes with SapTrap Even...

  2. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...to top Recombinase-Dependent Reporter Plasmids Incorporating a fluorescent protein into your expression...Plasmids Recombinase-Dependent Empty Backbones Reporter Plasmids Pooled Libraries/Kits Site-specific recombinase...recombinases , as well as useful empty backbones or reporters . Experimental Considerations Recombinase Recognition...DOG New Tricks: Controlling Protein Activity with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and...visualize which cells have undergone recombination. Reporter plasmids can also be useful for lineage tracing... Plasmid Collections AAV Enhancer Collection Fluorescent Biosensors Chemogenetics Plasmid Collection Optogenetics...

  3. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...low levels of both fluorescence and sequence overlap (coral mOrange2, jellyfish EGFP, and sea anemone mKate2...cells. The Brainbow-1.0 construct contains three fluorescent proteins: RFP (red), YFP (yellow) and M-CFP (...recombination. Brainbow-1.1 scales up the system to four fluorescent proteins, with recombination events specified...but the principles remain the same. The first fluorescent protein in the construct, OFP (orange), is expressed...-2.1 construct can express one of four colors (n-GFP, RFP, YFP or M-CFP.) The construct contains two tandem... tandem invertible segments, each with two fluorescent protein coding sequences in opposite orientations...expressed; once Cre is removed from the system, the fluorescent protein whose coding sequence is closest to the...

  4. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...transporters. The intensity-based Glutamate-Sensing Fluorescent Reporter (iGluSnFR) designed and improved by the ...optogenetic activator channelrhodopsin-2 or fluorescent reporter dTomato under the control of one of seven...primary visual cortex (V1) layer 2/3 (L2/3). B) Fluorescence from axonal boutons of neurons expressing iGluSnFR3...and labeled with primary antibody (0.01 µg/mL Anti-GFP [N86/38.1R] (Addgene #180084), 0.01 µg/mL Anti-HA...interneurons in mice. A) AAV expression of dTomato reporter (magenta) under the control of the BiPVe3 enhancer...Addgene cloned EGFP into pAG Lenti CMV N-HA Puro (Addgene #236079) to create pAG Lenti CMV HA-EGFP Puro (Addgene...excited to share our very own set of empty, HA-, and EGFP-expressing lentiviral backbones designed right here...

  5. Fluorescent Proteins: FRET

    Type
    Collection
    ... Plasmid Collections Fluorescent Proteins FRET Fluorescent Proteins: FRET FRET Pairs FRET Calibration...use FRET to report on protein binding or conformational changes. Organic dyes, fluorescent proteins, and...Check out our Biosensors Collection to find fluorescent reporters for metal ions, small molecules, and more...Calibration Standards Additional Resources Förster (or Fluorescence) Resonance Energy Transfer (FRET) is a process...experiments. FRET efficiency can be measured using fluorescence microscopy or spectroscopy by exciting the donor...emission of the acceptor. Alternatively, the fluorescence lifetime (the time between excitation of the...a photon) of the donor can be measured using fluorescence lifetime imaging microscopy (FLIM) or FLIM-FRET...

  7. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...quantity inside a cell Plasmids 101: Green Fluorescent Protein (GFP) Plasmids 101: Luciferase Monitor ...expression plasmids, gene knock-down plasmids, reporter plasmids, viral plasmids, and genome engineering...

  8. Zebrafish Plasmid Collection

    Type
    Collection
    ...for genome editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression plasmids...markers, somatic mutations, or labeling with fluorescent proteins to visualize and track clones of cells... Albert Pan and Alex Schier Labs. Multicolor fluorescent labeling in zebrafish Whole organism lineage ...studying diseases, are listed below: Lipid droplet reporters - Richard White Lab. An imaging platform to study...study lipid dynamics in vivo. Bimolecular fluorescence complementation (BiFC) tools - Marco Morsch Lab...Michel Bagnat lab. Targeting cassettes encoding fluorescent proteins for tagging genes at endogenous genomic...using red light. LipoGlo - Steven Farber Lab. A reporter system that allows direct assessment of lipoprotein...

  9. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...there is no fluorescence background resulting from nonspecific donor labeling. Fluorescent proteins (FPs...incorporation of fluorescent probes into protein: hexahistidine-tag-mediated fluorescent labeling with (...Experiments Beware of Fluorescent Protein Aggregation Brush up on Your Fluorescent Protein Basics Resources...Browse Empty Plasmids to Fluorescent Protein Fusions Check out Our Fluorescent Protein Guide ... permeabilized cells  Cy5NTAc 32 25-45 GFP (fluorescein is similar)   Lumio Red Tc: CCPGCC 48 38-70...answers. What is FRET and what's it good for? Fluorescence resonance energy transfer (FRET) is a biophysical...uncoupled donors. The best method for targeting a fluorescent donor to your protein is to hardwire the molecule...

  10. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...reproduced their EGFP findings since the initial report in 2023.)     Figure 1: Fluorescence intensity...a magnetoresponsive fluorescent protein. Controlling an optogenetic or fluorescent tool with magnets would... do fluorescent proteins respond to magnetic fields?” and “What are magnetoresponsive fluorescent proteins... expressing good ol’ EGFP — showed a slight but detectable change in fluorescence when a small magnet ...see if any other fluorescent proteins display a similar effect (many do). Fusing EGFP to the self-labeling...set out to do just that — by developing a new fluorescent protein tool that responds strongly to magnetic...Motivated by curiosity Although a wide range of fluorescent proteins and optogenetic tools exist, humans ...

  11. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...Libraries are available with T-Sapphire, EGFP, or mScarlet reporters, so you can choose a color compatible...in this post: Bright and photostable yellow fluorescent protein mGold2 Viral delivery of TnpB for plant...connections with ATLAS Bright and photostable yellow fluorescent protein mGold2 By Mike Lacy Get your sunglasses...these proteins are bright! Most current yellow fluorescent proteins tend to photobleach rapidly, which hinders...St-Pierre recently developed a pair of new yellow fluorescent proteins: mGold2s and mGold2t (Lee & Lai, et ...using mVenus or mCitrine, or if existing yellow fluorescent proteins just haven’t met your needs, consider...et al. (2025). Bright and photostable yellow fluorescent proteins for extended imaging. Nature Communications...

  12. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...along with a reporter, such as a fluorescence gene or a resistance gene. These reporters allow you to ...at equivalent levels upstream of an IRES fluorescent reporter for easy detection.5  Just released: Addgene's... using IRES elements. Further, scientists have reported lower expression of the downstream cistron due...eukaryotic cells, and, although some scientists report close to 100% cleavage with some of these peptides...co-express your gene of interest along with a fluorescent protein or selectable marker, it is easiest to...that already has the multicistronic element and reporter cloned in. In these plasmids you would simply ... 2A element (depending on the placement of the reporter gene). Addgene's collection offers a variety of...

  13. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...with Rosella, a pH-sensitive fluorescent biosensor A pH-sensitive fluorescent biosensor-based assay system..., Hess et al. successfully evolved wild type GFP to EGFP using CRISPR-X and subsequent FACS sorting. They...-ome using a quick, affordable, and ubiquitous reporter assay. In their recent publication, Kroeze et ...with unique tissue-specific promoters expressing GFP, tetracycline response elements, and shRNAs many ... of DsRed connected to a pH-sensitive variant of GFP (SEP) by a 9 amino-acid linker (see Figure 1). The...DsRed is relatively pH-insensitive, while SEP fluoresces at cellular pH (7.0) but not at vacuolar pH (...environments that inactivate SEP and leave only DsRed to fluoresce (1). With variants that can be targeted to specific...

  14. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ...approach to selecting a bright fluorescent protein and a photostable fluorescent protein. In the third post.... In corals, the red fluorescent proteins form tetramers. In general, fluorescent proteins have a natural...your research. But be aware that the fluorescent proteins reported in publications are only characterized... Addgene Blog Which Fluorescent Protein Should I Use? Choosing Your Fluorescent Proteins for Multicolor... with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire Fluorescent Protein...select a non-aggregating fluorescent protein. In the jellyfish Aequorea victoria, AvGFP forms a homodimer. ...be compared with mEGFP or mTurquoise2, which are established true monomeric fluorescent proteins. Concluding...

  15. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...relies on the fluorescence ratio between the 2 fluorophores, rather than on the fluorescence intensity, ...fluorophore (circularly permuted green fluorescent protein or cpGFP) to the petunia SL enzymatic receptor...the receptor which modulates the fluorescence intensity of the cpGFP without altering that of the internal...straightforward workflow for doing gene targeting, reporter generation, controllable gene knockouts, and inducible...thoughtfully designed plasmid components with Fluorescence Activated Cell Sorting (FACS), allowing for .../doi.org/10.1101/2020.05.04.076067    Novel fluorescent enzyme biosensors provide direct, specific and... Claudia Vickers’s lab has designed novel SL fluorescent biosensors by integrating a SL-insensitive fluorophore...

  16. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...sigh. The problem is that many of these studies report correlations without clearly testing whether a ...Addgene has several great options: pLV hUbC-dCas9-T2A-GFP for removable dCas9 expression and pLM-CMV-R-Cre....expressing these constructs can be achieved by fluorescence-activated cell sorting, and stable gRNA expression...

  17. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    ...explains how to critically assess the reported properties of fluorescent proteins, how to do a head-to-head...comparison of fluorescent proteins and how to make a well-informed decision on the best fluorescent protein ...best fluorescent protein for a specific application can seem like a daunting task. Many fluorescent proteins... number of fluorescent proteins is steadily increasing. Often, the choice for a fluorescent protein is...properties of fluorescent proteins on websites. To make the best choice, the fluorescent protein properties...Brightness Brightness refers to the fluorescence intensity of a fluorescent molecule. A higher brightness increases...tables with fluorescent protein properties and provides a quick way to compare fluorescent proteins, e.g...

  18. CRISPR Plasmids - Tagging

    Type
    Collection
    ... general cloning plasmid, and a prebuilt Unc-32::GFP targeting vector. Jorgensen Lab SapTrap CRISPR/Cas...with the following article: Natsume, et al. Cell Reports 2016 Förstemann Drosophila Cell Tagging System ...provide PCR templates for amplification of the tag (eg GFP, Flag, YFP, etc) and selection markers. Two independent... the first publicly available collection of fluorescently tagged, human, induced pluripotent stem cell...PITCh system plasmids for CRISPR-based knock-in of EGFP-2A-PuroR cassette to the C-terminus of endogenous...the donor vector. The PITCh donor plasmid with an EGFP-2A-PuroR cassette, flanked by microhomologous sequences...first deposited PITCh plasmids were tested by fusing EGFP-2A-PuroR cassette to a nucleolar protein, fibrillarin...

  19. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...incubation, you assess the level of fluorescence in your cells under a fluorescence microscope. The higher the ...green fluorescent protein (EGFP) reconstitution. (a) Scheme of validation for DSB mediated EGFP expression...where your target is inserted, disrupting the EGFP fluorescent signal, for details on this plasmid, see Mashiko...containing sgRNA sequences into HEK293T cells. The fluorescence intensity was classified into four groups (4...darker than control, 1; very dark). Brighter fluorescence indicates a more successful gRNA design. Adapted...the amount of fluorescence, the better that gRNA is at causing a double stranded break at the target site...screening as little as 8-10 pups. The level of fluorescence you saw in the validation assay should give ...

  20. Biosensor AAV Preps

    Type
    Collection
    ...KGC-GFP-ER2 Syn Archon1 EGFP Constitutive 8 Edward Boyden 115893 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2...Yulong Li Voltage Reporter: Archon 108422 pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 CAG Archon1 EGFP Cre dependent...KGC-GFP-ER2] Syn Archon1 EGFP Cre dependent 8 Edward Boyden Voltage Reporter: Voltron 119036 pAAV-hsyn-flex-Voltron-ST...Service: Biosensor AAV Browse our AAV that express fluorescent biosensor proteins, including calcium sensors...-ST none Cre dependent 1 GENIE Project Voltage Reporter: JEDI-2P 179459 pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE ...none Constitutive 9 Francois St-Pierre Voltage Reporter: ASAP 225707 pAAV-hsyn-ASAP5-Kv2.1-WPRE Syn ASAP5...HaloCaMP1a 138327 pAAV-synapsin-HaloCaMP1a-EGFP Syn HaloCaMP1a EGFP Constitutive 1, 9 Eric Schreiter Calcium...

  21. Tetracycline Inducible Expression

    Type
    Collection
    ... evaluate shRNA efficacy by fluorescence, use Plasmid #35626 as a reporter for cDNA target. rtTA3 Ptet...Iwasato 63704 pRetroX GFP T2A Cre Retroviral vector for dox-inducible expression of GFP T2A Cre recombinase...Eric Kowarz 96930 XLone-GFP Tet-On PiggyBac vector for inducble expression of EGFP Tet-On 3G rtTA TRE3GS...171123 pLVX-TetOne-Puro-GFP Lentiviral Tet-On vector for inducible expression of EGFP Tet-On 3G rtTA TRE3GS...pTet-IRES-EGFP Lentiviral plasmid for Tet-controlled expression of transgene of interest with EGFP (On or... Off) None TRE, miniCMV Maria Lung 16542 pBI-MCS-EGFP Bidirectional promoter (Pbi) for Tet-responsive ...expression (On or Off) of both your gene of interest and EGFP. Pbi contains a TRE between two minimal CMV promoters...

  22. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...measurable through an epitope tag such as FLAG/His/GFP. Fluorescent protein tags, which correlate closely with... cells were labelled with a green or violet, fluorescent dye, respectively. WT and KO cells were mixed... specific detection of human TRPA1. Scientific Reports, 9(1), 18500. https://doi.org/10.1038/s41598-019...use in western blot, immunoprecipitation, immunofluorescence and flow cytometry. F1000Research, 13, 817...

  23. CRISPR Guide

    Type
    Collection
    ...sequences using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the...catalytically inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable...viral genome, and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a...customizable DNA or RNA label for fluorescence microscopy. RNA aptamers, like MS2 or PP7, can also be fused to...to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging can...dCas9 and S. aureus dCas9) tagged with different fluorescent proteins or by fusing the gRNAs to orthogonal...imaging of genomic loci, as in the CRISPR-Sirius . Fluorescent CRISPR systems have been used for dynamic tracking...

  24. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ... the commonly used cassettes, such as fluorescent proteins (EGFP, mCherry etc.), recombinases (Cre, Flp... make. For example, large insertions, like a fluorescent protein knock-in, suffer from low efficiency ...with your desired insertion feature (e.g., a fluorescent protein), you can amplify the desired region ..., et al. "One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated...

  25. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...the only type of selectable marker. Fluorescent reporters, such as GFP, are another common selection marker...antibiotic, fluorescence-activated cell sorting (FACS) is used to sort cells expressing GFP and later, ...

  26. Modular Cloning Guide

    Type
    Guide
    ...Sieber Plasmids with control elements and fluorescent reporters to be used in combination with the MoClo-YTK...PhytoBrick parts. MoBiFC - Modular Bimolecular Fluorescence Complementation Toolkit Plant Expression Benjamin...Field 50 plasmids for assembling bimolecular fluorescence complementation experiments to analyze protein-protein...synthetic terminators, protein purification tags and fluorescence proteins for use in E. coli for a variety of...a combinatorial library of constructs expressing GFP at 18 levels for rapid characterization in new organisms...Christopher Dupont 95 plasmids including backbones, fluorescent proteins, protein tags, and signal peptides, ...

  27. Molecular Biology Reference

    Type
    Guide
    ...elements. These plasmids contain a reporter gene (e.g., luciferase or GFP) that offers a readout of the activity...the control of a non-bacterial promoter or a fluorescent protein to select or sort the cells by visualization...TALEN Plasmids and Kits , Genome Engineering Kits Reporter Plasmids Used for studying the function of genetic...level of transcription driven by that promoter. Fluorescent Protein Plasmids , Luciferase Plasmids Viral ...relies on the random incorporation of modified, fluorescently-tagged bases during in vitro DNA replication... DNA because the ultimate goal is to have a fluorescently-tagged nucleotide at each position in the DNA...DNA strands are separated by size, read using fluorescence detection, and the sequence outputted in the...

  28. Lentiviral Vector Guide

    Type
    Guide
    ...the only type of selectable marker. Fluorescent reporters, such as GFP, are another common selection marker...antibiotic, fluorescence-activated cell sorting (FACS) is used to sort cells expressing GFP and later expanded...

  29. Optogenetics Guide

    Type
    Guide
    ...of proteins) and sensors (genetically-encoded reporters of molecular signals). These areas have each since...plasmids allow cloning of protein coding sequences with GFP-LOVpep, cpPDZ, ePDZb and ePDZb1 as tags. pDawn/pDusk...activator FixJ in the absence of blue light. 470 Fluorescent Protein domains Dronpa based Light-inducible ...engineered from a green-to-red photoconvertible fluorescent protein 400 UVR8 domains UVR8 plant photoreceptor...

  30. CRISPR Guide

    Type
    Guide
    ...sequences using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the...catalytically inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable...viral genome, and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a...customizable DNA or RNA label for fluorescence microscopy. RNA aptamers, like MS2 or PP7, can also be fused to...to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging can...dCas9 and S. aureus dCas9) tagged with different fluorescent proteins or by fusing the gRNAs to orthogonal...imaging of genomic loci, as in the CRISPR-Sirius . Fluorescent CRISPR systems have been used for dynamic tracking...

  31. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ... as well as the type of transgene delivered: fluorescent proteins, optogenetic sensors, CRISPR elements...expression from mouse to primate neocortex. Cell Reports, 34(13). https://doi.org/10.1016/j.celrep.2021.108754...relatively even distribution of target gene expression (EGFP, in the depositing manuscript) across the brain....
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