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Showing: 561 - 580 of 897 results

  1. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...insect cells, they are most often expressed from a plasmid in bacteria (Ahmed et al., 2012). One challenge...Resources on Addgene.org: Browse Addgene’s Antibody Plasmid Collection Find antibodies for neuroscience research...

  2. Evolution of Lab Techniques

    Type
    Blog Post
    ... on Analyzing DNA Sequencing Results Check out Plasmid Cloning by PCR Resources on Addgene.org Check...Pages Find Molecular Biology Tools Analyze Your Plasmids by Restriction Digest ...

  3. AAV Production in HEK293 Cells

    Type
    Protocol
    ...of each plasmid needed to have a 1:1:1 molar ratio with 2 mg total DNA per CS5 Plasmid Plasmid Size (bp...conditions must be optimized. Plasmids for transfection: pHelper; pRC (Rep-Cap), plasmid expressing your gene ...molar ratio of each plasmid: 2000 μg / 24,961 bp = 0.08 μg/bp Therefore, for each plasmid we need: Sample ... 1.00 727.6 pHelper 11,854 1.00 1,185 Transfer Plasmid 5,842 1.00 584.2 Total bp 24,961 In total, we would... the total number of base pairs from all three plasmids, we can determine the total μg/bp we need to achieve...Needed: 949.8 μg / 1.0 μg/μL = 949.8 μL Transfer Plasmid: 0.08 μg/bp × 5,842 bp = 468.1 μg Volume Needed... 2% HI-FBS into a sterile 1 L bottle. Add each plasmid DNA into the bottle containing the Opti-MEM. Mix...

  4. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

    Type
    Protocol
    ...Modify your plasmid MCS or add short sequences using oligo overlap cloning.... Protocols Plasmid Modification...Modification by Annealed Oligo Cloning Plasmid Modification by Annealed Oligo Cloning You may also like... Restriction...Restriction Digest of Plasmid DNA DNA Ligation Bacterial Transformation Summary Oligo overlap cloning can...anytime you need to add a short stretch of DNA to a plasmid, such as: Changing a Multiple Cloning Site (MCS...

  5. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...Recombinant Antibodies You may also like... Antibody Plasmid Collection Antibodies Blog Posts Molecular Biology...suspension HEK293 cells with recombinant antibody plasmids using Polyethylenimine Max as a transfection reagent...General Considerations For antibody-expressing plasmids containing the SV40 origin of replication use ... SV40 large T antigen. For antibody-expressing plasmids containing the Epstein-Barr virus origin of replication...meter Reagents HEK293 cells Recombinant antibody plasmid DNA 500 mL vented flask 50 mL conical tubes Microcentrifuge... Transfer the PEI-MAX and recombinant antibody plasmid DNA sample to the biosafety cabinet and incubate...biosafety cabinet. Add 180 µg of recombinant antibody plasmid DNA to one tube of 6 mL BCD TFX. Cap the tube and...

  6. Science as Art

    Type
    Blog Post
    ... Source Lab Resources on Addgene.org Try the Plasmid Land Game ...

  7. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...You’ve gotten the plasmid encoding your protein of interest from Addgene, transfected it into your target...from a variety of vendors and you may even find plasmids encoding antibodies from Addgene that you can ...

  8. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...for future preclinical and clinical work. Find Plasmids for Disease Research on Our Special Collections... Check out Our CRISPR Guide Pages Find CRISPR Plasmids for Your Research Find Validated gRNAs for Your...

  9. General Transfection

    Type
    Protocol
    ...therefore, plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, ...protocol to transfect mammalian cells with your plasmid of interest. Protocols..., the plasmid should also be propagated in an endonuclease negative E. coli strain such as NEB stable....is prepared, transfect cells with a fluorescent plasmid using a variety of ratios. Check the cells 1–2 ...

  11. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ... the submitted sequences were a concern to the plasmid function. The steps of the training process gave...

  13. Finding Your Perfect Job After University

    Type
    Blog Post
    ...found out the job was with Addgene. I had used plasmids from this company before and recognised the logo...experiments over the phone or explain how various plasmids work. Most people who call or email me are scientists...

  14. Pouring LB Agar Plates

    Type
    Protocol
    ...also like... Plasmids 101 Blog Posts Mol Bio Protocols Cloning Protocols Introduction Plasmids can carry ...resistance gene on a plasmid allows researchers to easily isolate bacteria containing that plasmid from bacteria...resistance to that antibiotic - usually conferred by a plasmid carrying the antibiotic resistance gene. The following...effective at selecting only bacteria containing the plasmids of interest (for example, fewer satellite colonies...
Showing: 561 - 580 of 897 results