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We narrowed to 939 results for: ina

Showing: 561 - 580 of 939 results

  2. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ... by geneticists to study gene function. In this final installment, I’ll provide a brief introduction as...transgenes in different loci allows for genetic recombination so that the transgenes can be inherited on the...Engineering Posts Use FLEx Vectors for Site-Specific Recombination Resources at Addgene.org Browse Drosophila...

  3. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...log off for the day. 1:45 PM--Write final report. Work on a final report for a user research project I...help implement solutions for the stakeholders. Finally, software product managers test the solutions with...

  4. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ... approach, and conclusions (see above). Keep original files in a separate folder: You will often use...for a single use, save the script as originalName_descriptionOfEdit in the same directory where it was ...that was temporarily used. Just be sure that the original code remains in tact! Take notes Comment in ...

  5. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...insert targeted mutations through homologous recombination. To further increase the utility of CRISPR/Cas9...containing the RNA pol III promoter, gRNA and pol III terminator. These relatively large cassettes (considering...Size is also a factor; the pol lll promoter and terminator need be encoded only once, and each <200 bp tRNA-gRNA...

  6. The time and cost required to make a plasmid

    Type
    Blog Post
    ...This post was originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered...cloning, waiting, and repeating before you can finally dive deep into generating the data you’re excited...cost $155.25 in reagents and consumables today. Finally, this doesn’t account for the most valuable component...

  7. Summer SciComm: Preprints

    Type
    Blog Post
    ...or awaiting a final publication in a journal.  Preprints can be one tool in a combination of strategies... Addgene ID for their deposited plasmids in the final journal publication or an updated preprint, further...

  9. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...ligation assay (PLA). The clever combination of FUNCAT and PLA PLA was originally published by the Söderberg...16590564. PubMed Central PMCID: PMC222790. 6. Buxbaum, Adina R., Bin Wu, and Robert H. Singer. "Single β-actin...

  11. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...yellow, or magenta, depending on the hairpin combination bringing the total number of individual colors...Adding an additional hairpin/fluorescent protein combination, for example, with a far-red FP, would increase...15! They also envision using CRISPRainbow in combination with gene editing. CRISPRainbow requires very...

  12. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    ...available (below). However, on November 22nd a preliminary injunction against the FLSA updates was granted...inconsistencies across geographical regions When we originally started gathering this information, many assumed...to pursue academic work, and to enhance the sustainability of the scientific enterprise by reducing the...

  13. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...questions the conclusion of inter-homologue recombination was released. This blog has not been updated...al. were interested in correcting an autosomal dominant mutation in MYBPC3 that leads to hypertrophic ...embryos to find those that do not carry a given dominant disease-causing gene, or that carry only one copy...

  14. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ... deletions.   Cas12a – The staggered cutter  Originally classified as Cpf1, Cas12’s primary difference...Another similarity to Cas12a is that Cas14 also indiscriminately cleaves non-complementary ssDNA when engaged... the genome. Cas13, similar to Cas14, also indiscriminately cleaves ssRNA when engaged at its RNA target...

  15. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...drug discovery process.” The ClonTracer library originally came about as Bhang and co-workers were trying...treatment process. The authors went on to show that combination treatment with multiple drugs reveals even smaller...heterogeneity to drug discovery and the development of combination therapies.         References 1. Hyo-eun, C. ...

  16. Design Tips for Prime Editing

    Type
    Blog Post
    ... Editing, and that meant rereading many of the original papers reporting various prime editing tools. ...your curiosity, we encourage you to check out the original papers for more details. You can find many software...prime editing systems by manipulating cellular determinants of editing outcomes. Cell, 184(22), 5635-5652...

  17. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...AAV serotypes, and the ever-growing variety of recombinase systems. Picking Ligands Picking the right ligand...ligand co-developed by Bryan Roth's and Takafumi Minamimoto's lab called deschloroclozapine (DCZ) shows promise...our chemogenetics poster! This blog post was originally written by Maya Peters Kostman in May 2020 and...

  18. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...dCas9. Within a few rounds of cell division, the original methylated strand is diluted to undetectable amounts...dCas9 so that it may later be excised by Cre recombinase. Addgene has several great options: pLV hUbC-...L, Li C, et al. (2018) OGT binds a conserved C-terminal domain of TET1 to regulate TET1 activity and function...

  19. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...Originally published Apr 30, 2020 and last updated Jul 12, 2021. What started out as a small collection...used for this study at Addgene which include a C-terminal deletion in the spike protein to improve expression...pseudoviruses to determine how effective sera from vaccinated individuals are at neutralizing the different...

  20. Lab Automation at Addgene

    Type
    Blog Post
    ...is ideal because it eliminates the potential of human error or cross contamination between samples. With... Shannon Rinaldi...
Showing: 561 - 580 of 939 results