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  1. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ... a crowd. Having answer slides ready after your formal conclusion slide will also help with your confidence...with other ways to engage the audience. Take an informal poll by asking for a show of hands on a particular...

  4. A Control for All Seasons

    Type
    Blog Post
    ...antibody is replaced with pre-immune serum from the animal the antibody was produced in or an isotype-matched...primary antibody is incubated with its antigen or a small epitope-containing peptide to block the antigen-...

  5. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...of endogenous light-sensitive receptors, like mammalian rhodopsin, and the intracellular domain of an ...experiments.  Wavelengths Most vectors have an optimal wavelength to use but can be activated by a range...

  6. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...), but there are differences in tropism between animal species or even strains of the same species. What... to define high titer, then, is this: It’s the optimal number of viral particles per volume required to...

  8. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...in the uncut DNA; the rest of the digest appears normal and produces the expected fragments for that plasmid...transfected supercoiled plasmid DNA concatemers in mammalian cells. J Gene Med. 2009 May;11(5):444-53. doi:...

  9. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...of one AAV serotype with the capsid of another. Formally, pseudotypes are described with two numbers separated...using the genomic structure of AAV2, so they are formally called rAAV2/1, rAAV2/2, and so on, although we...

  11. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...collaborative learning style at all. Finally I rotated in a small lab with a new PI. I realized that as a first born...collaboration? If you are considering a career at a small, teaching college you might want to consider less...

  13. Finding Your Perfect Job After University

    Type
    Blog Post
    ... agencies and noticed a position available at a small science non-profit doing customer service (recruitment...this is a fairly unique situation, because it is a small and dynamic company and so it has the flexibility...

  14. Finding Your Science Policy Path

    Type
    Blog Post
    ...societies, and industry through convening activities, formal lobbying, or strategy setting.  The “little p” ...question may change over time and that’s perfectly normal!  Action Items:  Start a career journal during...

  15. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...Moreau (1896) where he explores the concepts of animal testing and modifications. The premise of the novel... novel involves Dr. Moreau who creates human-animal hybrids. This book is published 30 years after the...

  17. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...development of viral vectors, gene transfer into mammalian cells often relied on chemical (CaCl2, cationic...If not using proper technique, there’s always a small chance that a researcher could infect him or herself...

  18. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...found that many researchers feel that they lack formal training in a variety of transferable skills. At...until the team really starts functioning (or malfunctioning). Clear lines of communication Few people actually...

  19. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...antibodies to pass through the cell membrane. Formaldehyde fixation creates cross-links between lysine ...Sometimes, users will combine methods and fix with formaldehyde first to freeze everything in place followed...
Showing: 561 - 580 of 814 results