...Brainbow-1.0 in that transient Cre recombination is required to prevent constant inversion of the construct...derivatives of the fluorescent proteins, which are directly trafficked to cell membranes. Membrane trafficking... Fouquet S, Dupin E, Benosman R, Chédotal A, Beaurepaire E, Morin X, Livet J. Neuron. 2014 Feb 5;81(3)...

...advantage to using direct epigenetic modifiers? As with any experiment, your desired outcome will determine... DNA sequences. Non-editing CRISPR applications direct catalytically dead dCas9 fused to a variety of ...

... in a DNA double strand break (DSB), the event required for frameshift and knock-in mutations. If one ...will generate a ssDNA break which can easily be repaired by cells with a near zero likelihood of mutation...sequencing The only way to quantify off-target effects entirely is to perform whole genome sequencing. While this...

... Is Bitter, but Its Fruit Is Sweet Time If you require a quick onset of expression, AAV may not be the...AAVs for DREADD delivery indicates ~7-21 days are required for the onset of gene expression. However, it ... As single stranded DNA viruses, AAVs normally require second strand synthesis before gene expression ...

...variable region of an antibody does not recognize the entirety of the target protein: instead, it recognizes ...between the IgG antibody and scFv. Because the entire antibody molecule is not necessary for antigen ...other markers can be injected into an animal, to directly label circulating cells or other cells that are...

...antigen of interest in the given protocol. This requires use of one of the five pillars of antibody validation...intended application — here, flow cytometry — is the desired target, using one or more of the approaches outlined...relative protein expression. Its reliability will be entirely dependent on the selectivity of the antibody used...

...and CSO Melina Fan's words of welcome! Executive Director Chonnettia Jones and Board Member Joanna Brownstein...each and every Addgenie, from our co-ops to our directors, we can't celebrate Addgene without recognizing...

...decision. We realize that with this move, we lose a direct connection with our nearly 22,000 X followers. ...official Addgene account. You can reach out to us directly via email at [email protected], and we invite you...

...John Jumper! The Baker Lab focuses on designing entirely new proteins, improving our understanding of how...John Jumper! References Kuhlman, B., Dantas, G., Ireton, G. C., Varani, G., Stoddard, B. L., & Baker, D...

...around the corner. Here at Addgene, we’re always inspired by the new seasons, so we've made some big summer...Landscape for Engineered Systemic AAVs” with the director of CalTech’s CLOVER lab, Tim Miles, PhD, we invite...

...Collins labs at the Wyss institute for Biologically Inspired Engineering. Alex is the first MD PhD we've had... - 13:21 : The Wyss Institute for Biologically Inspired Engineering and why Alex decided to work there...

...Components of CRISPR: gRNAs: Sequences of RNA that direct the CRISPR system to cut other DNA or RNA sequences...Nucleases: Proteins that bind to gRNAs and are directed by the gRNAs to cut particular DNA or RNA sequences...

...Matthew J. Sikora (@mjsikora) December 18, 2018 Fireplace from the Dyer Lab If you’re feeling as chilly ..., they turned one of the lab computers into a fireplace scene.  Got our @EmoryChem lab office looking...

...below the ordering table. Click on it, select your desired serotype and volume, and submit your request.  ...simply request your plasmid of interest in your desired serotype, place your order when ready, and we’ll...

...Increased Postdoc Wages,” Future of Research executive director, Gary McDowell, gives a thorough yet concise rundown...postdocs various academic institutions can afford to hire. It will be difficult for some of these institutions...

...would be an average of 673 plasmids each day. This requires tremendous coordination amongst the lab team, ...above), but as Joanne Kamens, Addgene’s executive director, said during our celebratory lunch, it’s hard ...

...biomass. Dr. Duedu would like to use the plasmids he acquires through Addgene to produce molecular biological...PCR. We hope these plasmids will help Dr. Duedu inspire new Ghanaian scientists to continue to do excellent...

...insert are often denoted by "wt" either directly before or directly after the insert name.   Example: If ...

...Cambridge before spending time in biotech screening direct interactions between drugs and promoters of oncogenes...and extremophiles for biotechnology and has been directly involved in supervising many successful iGEM undergraduate...

... an introduction to Addgene with our Executive Director, Joanne Kamens. As a regular contributor of careers...teams at Addgene. An introduction to plasmids with Director of Biology Lianna Swanson was very popular, with...