We narrowed to 611 results for: sam

1. Viral Vectors 101: Optogenetic Tools

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   Blog Post

   ...one cell type to another, as they may not use the same pathways. Additionally, as GPCR signaling is amplified...

2. Finding Your Perfect Job After University

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   Blog Post

   ...cancer and I loved the practical aspects of testing samples in the lab, but I really struggled with the large...

3. Building Global Connections with the International Mentorship Program USA-EUROPE

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   Blog Post

   ... my mentor, since she does research at UK in the same field that I do: prostate cancer. She has guided...

4. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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   Blog Post

   ... bottom of the gel in the BRAF gRNA-transduced samples (data not shown). Pro-Tips: 1.Designing primers...

5. RNA Interference in Plant Biology: New Tools for an Old Favorite

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   Blog Post

   ...between gene silencing and knockout methods of the same gene. However, recent work has called this prevalent...

6. A History of Genome Engineering in Popular Culture

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   Blog Post

   ...findings of the 1952 Hershey-Chase experiment. In the same year, Rosalind Franklin and her student Raymond ...

7. Sequencing Options for CRISPR Genotyping

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   Blog Post

   ...investigator can be relatively certain that their samples don’t have off-target mutations if they remain ...

8. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

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   Blog Post

   ...changes 5’ to the PAM, sequences corresponding to the same strand as the PAM produced a higher editing efficiency...

9. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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   Blog Post

   ...depth. Two low-energy photons (typically from the same laser) cooperate to cause a higher-energy electronic...

10. Cancer and the Immune System: Deciphering the Relationship

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    Blog Post

    ...multiple immunotherapy targets can be utilized at the same time. A combination of tolerance marker blockade...

11. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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    Blog Post

    ...turnaround time 5-7 days); because the tracrRNA is the same in all csRNP experiments, it can be ordered in bulk...

12. Plasmids 101: Modular Cloning Applications and Kits

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    Blog Post

    ...unique genomic targets or multiple sgRNAs against the same target to ensure efficient cutting. Beyond sgRNAs...

13. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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    Blog Post

    ...human induced pluripotent stem cells from urine samples. Nat Protoc, 2012. 7(12): p. 2080-9. PubMed PMID...

14. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

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    Blog Post

    ...likely because both innovations aim to solve the same problem. Both approaches are effective, but users...

15. Optogenetics + CRISPR, Using Light to Control Genome Editing

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    Blog Post

    ...light-inducible system was able to recognize the same PAM and had similar targeting specificity as full-length...

16. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

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    Blog Post

    ...https://doi.org/10.1038/nbt.3043 Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA (2013) Programmable...

17. 27 Hot Plasmids from 2016

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    Blog Post

    ...population within a given tissue rather than a mixed sample. TRIBE was developed in Drosophila, and further...

18. Chemogenetics Guide

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    Guide

    ...arePharmacologically Selective Actuator Modules (PSAMs, pronounced SAMs). PSAMs are engineered α7 nicotinic acetylcholine...varenicline. This PSAM carries mutations at L131G, Q139L, and Y217F and is termed PSAM4 (or PSAM 4 ). Additional...cognate PSEM. For example, PSAM Q79G,Q139G is activated by PSEM 22S , while PSAM L141F,Y115F is activated... effort to develop a PSAM that responded to a clinically approved drug, the PSAM domain was engineered...have also been developed as agonists for PSAM4. A given PSAM is coupled with an ion pore domain (IPD) ...signaling. PSAM-based LGICs can be classified by the ion pore domain they are coupled to: PSAM-Gly LGICs...ions, and inhibits neuronal activity. PSAM-5HT3 LGICs pair a PSAM domain with a 5-HT 3 serotonin receptor...

19. Plan Your Experiment

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    Guide

    ...activator systems exist, including the multi-plasmid SAM system Activate Design and Clone Your gRNA Achieving...the genomic region you intend to target in your samples to ensure there are no discrepancies with the reference...

20. AAV Titration by qPCR Using SYBR Green Technology

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    Protocol

    ...empty NTC C AAV reference Sample 3 D E Sample 1 Sample 4 F E Sample 2 Sample 5 F Perform data analysis...most samples. If sample is expected to have a titer <1 x 10 12 GC/mL, use dilutions 3–6 If sample is expected...standards and samples in duplicate at least If possible, include an AAV reference sample of known titer...Count the number of samples (n) and prepare master mix for an additional 10 samples (n+10 – the additional...that there is enough master mix for all samples). Each sample requires 15 μL of master mix. Pro-Tips Use...pipetting error and sample evaporation. Make the master mix after all the samples have been added to the...virion). 5 μL sample + 39 μL H 2 O + 5 μL 10X DNase buffer + 1 μL DNase Gently mix sample (do not vortex...