... both on and off target. Across all loci tested, error rates overall ranged from 0.3% to 10%, in comparison...Importantly, this is true in both mice and nonhuman primates (for AAV.CAP-B10, for example).  Given that the...

...information, making it available to everyone, that creates more breakthrough and innovation. With any kind...Suffolk University Law School. Suffolk University graduates on average 20 PhD’s a year and we have a very ...Committee at the Greater Boston Food Bank and participates locally in Read To A Child's "Lunchtime Reading...

...calculated (details in [2]) and can be used to quantify rates of mutation in a bulk or single cell-derived genomic...instructions on how to design such assays, in addition to testing their performance (2). Remember that although digital...transfection of a novel TAL effector nuclease (TALEN) facilitates efficient knockout of HIV co-receptor CCR5. Nucleic...

...be used to express proteins. Viral expression cassettes typically contain a promoter (inducible or constitutive...lineage, and so much more! Libraries allow you to test thousands of elements simultaneously in your system...dose. Vaccine development strategies. Image courtesy of Gorry et. al, 2007.   Clinical applications...

...to the right indicates a higher signal, or upregulation, and a peak to the left indicates a lower signal...continue gating populations of interest with normal gates, just based on a histogram plot instead of a dot...

... packaging cell line. This system provides the greatest flexibility to pseudotype γ-retrovirus using different...post‐transcriptional regulatory element; sequence that stimulates the expression of transgenes via increased nuclear...U5 and the rest of the provirus. 3’ LTR Terminates transcription started by 5' LTR by the addition...

...companies or check the literature. Pro tip! Early gates, like your live/dead markers or commonly used markers...specific cell populations, often used in the later gates, are more likely to require FMOs. Isotypes Isotype...rather than for setting up your initial voltages and gates. Ashlyn has a research background in CD8+ T cells...

... trans activity. AsCas12a, on the other hand, generates staggered DNA nicks at its target in cis and cleaves... used by Jinek et al. 2012, or browsing for the latest AsCas12a plasmids, Addgene and CasPEDIA have got...from CasPEDIA.   How can I use it? CasPEDIA operates like many search engines, featuring both a general...

...together. I also prefer the number system to using dates as labels, since I often work on multiple projects...sure that the original code remains in tact! Take notes Comment in your scripts: Comments on what a given...who are still learning the basics. However, these notes take up a lot of space and don’t tell you why you...

...infancy. A long timeline from transformation to testing and a lack of well-characterized genetic tools ...excited to see how this exchange of genetic tools facilitates plant science. Challenge #3: Intellectual property...in 2004, she accepted a commission in the United States Air Force. She left active duty to pursue a degree...

...keep track of how you met the connection, as these notes are saved in the messaging stream. Unfortunately...LinkedIn is not the platform for politics, family updates, or click bait of any kind. For more on inappropriate...However, do comment on other people’s news and updates. Share your own good ideas, events and favorite...

...large number of targets, and can require specific conjugates. And, of course, once you have them, you’ll need... along with a higher likelihood of aggregation (Bates, 2019). They are small enough to be produced in ...delivery systems. Elsevier Science & Technology Books. Bates A, Power CA. David vs. Goliath: The Structure, Function...

...options. With limited time and budget you cannot test them all, so how do you know which ones to try?  ...immune complexes are smaller than a primary:secondaries:conjugates complex. If you are trying to visualize....  The 5 pillars include genetic strategies that test expression in knock-out or knock-down down tissues...

...with the desired resolution of prime editing intermediates. Building upon the PE2 and PE3 systems of their...introduce epitope tags and RNA transcription start sites. The lab further demonstrated that PRIME-del can...RT), which induces two double-strand breaks and deletes the intervening sequence. As with other prime editing...

...CASANOVA responds to blue light quickly. Within minutes of inactivating the CASANOVA complex, fluorescent...genes-of-interest (GOI) into two independent expression cassettes on a single plasmid (Grefen and Blatt, 2012). ...proteins that bind to either the light- and dark- states of the photoswitchable cPYP, which changes in conformation...

...caveolin 1, also known as CAV 1. Usually, caveolin 1 promotes the cell life cycle progression and enhances tumor... TAK 1, an enzyme that controls apoptosis and regulates transcription. Without TKA1, cells proliferate... 2004). In fact, Sanford H. Barsky and his team tested the influence myoepithelial cells had on the conversion...

... oxygen concentration may affect photobleaching rates (Shaner et al, 2008). This brings us to the key ...application (Goedhart et al, 2012). The third issue relates to the environment of the fluorescent protein. ...The issues with quantification of photobleaching rates can be summarized as follows: Instead of determining...

... cross-talk with the other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric...individual colors to 6. A gRNA with all three hairpins generates white light. All 6 gRNAs can be expressed in a...separate vector. Figure 2: CRISPRainbow generates 6 unique colors and a white composite. gRNAs with...

...a new legal minimum of $47,476 per year, under updates to the Fair Labor Standards Act (FLSA). This was...22nd a preliminary injunction against the FLSA updates was granted nationwide. Again, we at Future of ...getting paid, which we will be analyzing for future updates. But suffice it to say, salaries are showing surprisingly...

...accelerating science! Photo courtesy of under a CC license. Many scientists and advocates have argued that while...manuscript is drafted. Preprints were one of the key routes for researchers to share information about the ...