...tasks, both of which are factors in the technical challenges they can present. Many researchers find themselves....2 (2003): 141. PubMed PMID: 12705861. 3. Chen, Sifeng, et al. "Freeze-thaw increases adeno-associated...

...Hongkui Zeng Additional Resrouces on the Addgene Blog  Read about Other Means of Genome Engineering Using...

...real-time, posing a huge challenge to studying epigenetic changes over time.  This challenge was difficult to ...

...Makerspace is composed of computer scientists, engineers, and biologists working together on a variety ... case.   Funding Makerspaces One of the main challenges when starting a new project at a makerspace is...

... of your supervisor’s management style, bring challenges to his or her attention as soon as you can. It...mutually beneficial solutions. If you package your challenges with your supervisor into learning opportunities...

...discussions with both panelists and audience fully engaged. They can also be dull and painful to sit through...well-planned approach to making the event interesting and engaging. Long before the event... Choose a moderator ...

...experiments, and prepared your reagents. You’re going to engineer the best thing since CRISPR; you are ready to ...technologies currently available, you now face the challenge of trying to figure out what system might work...

...post was contributed by Greg Dingle, a software engineer with the Chan Zuckerberg Initiative. We hereby...blogger, Greg Dingle! Greg Dingle is a software engineer with the Chan Zuckerberg Initiative, working on...

...entire time talking to the poster, as opposed to engaging with you? Be sure to use your poster as a visual...they are following along. This will not only re-engage you with those who sought out your poster, but ...

...and protein turnover rates. Additionally, by engineering FP fusions associated with cellular organelles...the tag for each insertion. Also, it remains challenging to screen for the genetically modified strains...

...natural. Creative negotiation skills will help you strengthen relationships with your colleagues and supervisors...help you get more of what you want and can also strengthen your relationships with others who know that ...

...identified in nature, while many more have been engineered in labs. This vastly increases the possibilities...S. E., Muller, A., Sousa, A. A., Tsai, S. Q., Bengtsson, N. E., Lööv, C., Ingelsson, M., Chamberlain, ...

...Come From and What Do They Mean? Overcoming the Challenges of Lentivirus Production Tips for Titering Your...recombinant proteins and viral vectors. Frontiers in Bioengineering and Biotechnology, 9. https://doi.org/10.3389...

...determinants of prime editing efficiency such as the length of the RTT overhang, the GC content of the primer... pridict.it. This approach leverages the strengths of PRIDICT/PRIDICT2.0 while accounting for some...

...mouse knockout lines should increase. New genome engineering tools also offer hope for the creation of new...models in species that were previously difficult to engineer, like rats. If you have tools for creating knockout...

...daily, yet obstacles remain. One of the biggest challenges facing researchers today is the complexity of... of the latest resources? In response to this challenge, Addgene has launched an initiative to curate ...

...and other genetic elements using specifically engineered plasmids, commonly referred to as vectors, which...promoter (e.g., a liver-specific promoter). The strength of the promoter is also important for controlling...that can drive expression of short RNAs. Genome Engineering Plasmids - Used to target and edit genomes. Genome...Strain Vendor(s) Genotype BL21 Invitrogen; New England BioLabs E. coli B F dcm ompT hsdS(rB mB) gal ccdB...traDelta36 proAB lacIqZDeltaM15] NEB Stable New England Biolabs F' proA+B+ lacIq ∆(lacZ)M15 zzf::Tn10 (...group of newly synthesized DNA strands of varying lengths whose last nucleotide is labeled. Once all the ...

...major players in the CRISPR field. Just a year after Feng Zhang’s lab identified Cas13a (C2c2) (Abudayyeh ...) displayed robust editing with various spacer lengths from 30-84 nucleotides, and designated this system...

...). Additional resources on the Addgene blog Engineering the plant genome using CRISPR/Cas9 Using pSiM24...pSiM24 for simplifying plant genetic engineering Using arabidopsis in the classroom Resources on Addgene.org...

...bypassing these issues. CRISPRi libraries utilize an engineered Cas9, which contains multiple point mutations...Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex." Nature517.7536 (2015): ...