We narrowed to 602 results for: sam

1. Adeno-associated Viruses (AAVs) for Genome Editing

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   Blog Post

   ...require modifying multiple genes simultaneously in the same cell, are more appropriate for a CRISPR-based approach...

2. March for Science

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   Blog Post

   ...politician Rush Holt said, government must have the same fundamental attribute that enables science: “humility...

3. Multiple Plasmids at a Low Price: Kits from Addgene

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   Blog Post

   ...plasmids used to generate these fusions all use the same restriction enzymes for cloning. Furthermore, some...

4. 10 Steps to a Perfect Science Talk

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   Blog Post

   ... it perfectly fits the occasion.  I speak on the same topics many times, but I always seek out information...

5. Treating Muscular Dystrophy with CRISPR Gene Editing

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   Blog Post

   ...slightly different methods, they each observed the same positive phenotypes in cardiac and skeletal muscle...

6. Thoughts on the Future of Research 2015 Boston Symposium

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   Blog Post

   ...underrepresented groups under their mentorship with the same critical eye they place on others within their labs...

7. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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   Blog Post

   ...three loxP variants, but the principles remain the same. The first fluorescent protein in the construct,...

8. Plasmids 101: Common Lab E. coli Strains

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   Blog Post

   ...convoluted due to researchers sharing and renaming samples throughout history. It was likely isolated in 1918...

9. Viral Vectors 101: Optogenetic Tools

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   Blog Post

   ...one cell type to another, as they may not use the same pathways. Additionally, as GPCR signaling is amplified...

10. CRISPR 101: Epigenetics and Editing the Epigenome

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    Blog Post

    ...activate transcription. A lentiviral vector with the same modifier, Fuw-dCas9-Tet1CD, is available from Rudolf...

11. Hot Plasmids: Spring 2025

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    Blog Post

    ...proteoglycans composed of a protein core, 2–4 glycosaminoglycans (GAG), and are (typically) tethered to the...

12. CRISPR 101: Off-Target Effects

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    Blog Post

    ...If you are performing CRISPR library screens, the same principle holds true - the higher the number of ...

13. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

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    Blog Post

    ...They have successfully used guides targeting the same or complementary strands, as well as guides facing...

14. Finding Your Perfect Job After University

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    Blog Post

    ...cancer and I loved the practical aspects of testing samples in the lab, but I really struggled with the large...

15. Building Global Connections with the International Mentorship Program USA-EUROPE

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    Blog Post

    ... my mentor, since she does research at UK in the same field that I do: prostate cancer. She has guided...

16. Plan Your Experiment

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    Guide

    ...different activators exist, including the multi-plasmid SAM system. Select Expression System To use CRISPR, you...efficacious than gRNAs containing a C nucleotide at the same position in spite of being a perfect match for the...

17. A History of Genome Engineering in Popular Culture

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    Blog Post

    ...findings of the 1952 Hershey-Chase experiment. In the same year, Rosalind Franklin and her student Raymond ...

18. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

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    Blog Post

    ...changes 5’ to the PAM, sequences corresponding to the same strand as the PAM produced a higher editing efficiency...

19. RNA Interference in Plant Biology: New Tools for an Old Favorite

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    Blog Post

    ...between gene silencing and knockout methods of the same gene. However, recent work has called this prevalent...

20. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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    Blog Post

    ... bottom of the gel in the BRAF gRNA-transduced samples (data not shown). Pro-Tips: 1.Designing primers...