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  1. Evolution of Lab Techniques

    Type
    Blog Post
    ...restriction mapping may still be used to screen multiple colonies before selecting a smaller subset to ...
  2. Finding Your Science Policy Path

    Type
    Blog Post
    ...simple question is a moving target. There are a multitude of options for achieving success in a science ...
  3. CRISPR Guide

    Type
    Guide
    ... Many different activators exist, including the multi-plasmid SAM system. Select Expression System To ...more about Cas9 multiplexing and Cas12a (Cpf1) multiplexing . Browse Plasmids: Multiplex gRNA vectors Genome-Wide...two gRNA target sites Modifying multiple genes at once Current multiplex CRISPR systems enable researchers...genetic loci by cloning multiple gRNAs into a single plasmid. These multiplex gRNA vectors can conceivably...in living cells. Multicolor CRISPR imaging allows for simultaneous tracking of multiple genomic loci in...Base Edit Activation or Repression Epigenetics Multiplex Genome Engineering Genome-wide Screening What ... Both types of base editors are available with multiple Cas9 variants including high fidelity Cas9’s. ...
  4. Adenovirus Guide

    Type
    Guide
    ...double-stranded DNA (dsDNA) molecule containing multiple, heavily spliced transcripts. At either end of...harvested about 7-10 days later. Vogelstein designed multiple shuttle vectors for different purposes. The pAdTrack...that the infection rates are equivalent across multiple viruses. The adenoviral backbone pAdEasy-1 is ...
  5. Guide to Using Pooled Libraries

    Type
    Guide
    ... unique gene targeting sequence, but there are multiple sequences targeting each gene in the overall library...lineage tracing or parsing the effects of expressing multiple genes at once. A well-designed screen can help...library screens, cells are infected at a very low multiplicity of infection (MOI) to increase the odds that...
  6. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ... strand. Rep and Cap are translated to produce multiple distinct proteins (Rep78, Rep68, Rep52, Rep40 ... experimented with hybrid capsids derived from multiple different serotypes, which also alter viral tropism...
  7. Cloning

    Type
    Guide
    ... same sites in the same orientation within its multiple cloning site . Due to their short length, it is...cloning is that it allows for the simple assembly of multiple fragments of DNA in the chosen orientation, and...
Showing: 521 - 540 of 544 results