...as examples for this post. The D10A mutation inactivates the RuvC domain, so this nickase cleaves only...and images in this post were adapted from an IDT webinar: Optimized methods to use Cas9 nickases in genome...For more information and a link to the archived webinar, click here. We would like to thank IDT scientists...

...significant improvement and simplification of the original technique, specifically in terms of initial steps...excellent additional measure of DNA binding sites in combination with existing ChIP-seq data sets, of which there... a graduate student at the University of North Carolina in Chapel Hill, where he studies enhancers and...

...then in my final year of graduate school, I actually served as the student course coordinator. With the...’d put in outside the lab had paid off. “The combination of the editing and teaching experience that I...

...perfectionists, it took us a full two months to finally decide the LEGO laboratory was good enough. There...box, the Big Bang Theory set, and then use our imaginations to mold it all into a full laboratory. It is...It was a great treat for us to put our rusty imaginations to use in this world where we are often trained...

...outside of academia, a job as a ‘Cancer Marketing Coordinator’ came up at Abcam. Unlike other job descriptions...scientists think that they are unable to tick these final two boxes, but I knew there would be a way around...marketing gives you a great introduction to the financial world. It teaches you about selling products which...

...This is the fifth and final post in the Addgene Blog Mentoring for Scientists Series. The entire series...SMART.” SMART goals are Specific, Measurable, Attainable, Relevant, and Time-bound. It is a good idea ... open communication Feel valued by giving back Final tips for success Make mentoring meetings a priority...

...Washington University in St. Louis provides a combinatorial indexing strategy that simultaneously tracks...cell type that can differentiate into hepatic or intestinal cell types. MEFs were reprogrammed into iEPs ...2019). Key takeaways of CellTag CellTag is a combinatory cell-indexing tool that combines scRNA-seq and...

...Di Ventura (@barbara_synbio) May 13, 2021 The original AraC transcriptional regulator Their system is...does BLADE have over AraC? When compared to the original AraC system, BLADE is simply more precise. “We...light switch and shuts off just as quickly. The original AraC system, which uses arabinose, requires many...

...mobilized to figure out how to deliver when labs finally opened.  I was awed by the teamwork and initiative...One function that rose to the challenge was our Finance Team. With Addgene closed for almost two weeks ...for unknown amounts of time, we were facing a financial crisis. As a nonprofit organization, Addgene charges...

...unsuccessful. Nothing more joyful that getting to the final step and the transformed bacteria refuse to grow...(Jackson ImmunoResearch, 016-050-084; 0.2 μg/ml final). Both rabbit (EMD, AB3596) and mouse (NEB, E8032S...matrices, not so much. Maybe a calcium-independent proteinase site could be used for plasmid version 2.0? Instruments...

...how I got my current job as a Communications Coordinator. Wondering how you can develop more transferable...Theresa Liao (@theresaliao) is the Communications Coordinator for the Department of Physics & Astronomy, University...University of British Columbia. Originally on track for a research career, she transitioned into a communications...

...Japanese ostracod(Cypridina noctiluca)Sea firefly(Cypridina hilgendorfii) 465 62 Cypridina (Vargulin) luciferin...

...considered an entirely new cell line from that of the original parental cells. Positive selection in mammalian...  HeLa, NIH3T3, CHO, COS-1, 293HEK Inhibits termination step of translation 2-10 ug/mL G418/Geneticin... Inhibits protein synthesis; premature chain termination 1-10 ug/mL Zeocin Sh bla HeLa, NIH3T3, CHO,...

... sign up here.        New toolkit for N or C terminal tagging of proteins for degradation using AID ...created a new toolkit of plasmids for the N- or C- terminal tagging of proteins with mAID and other tags (...SpCas9-NG with the activation-induced cytidine deaminase  Target-AID-NG, which mediated a C to T conversion...

...Generated against full length GFP and converted to recombinant form by the NeuroMab Facility Isotype IgG2a ...a polyclonal, with the reproducibility of a (recombinant) monoclonal. Another reason is that some antibodies...  et. al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex ...

..., cyan, or magenta) depending on the hairpin combination. A gRNA containing all three hairpins produces...generates blue, green, or red colors for labeling. A combination of MS2, PP7, or boxB sequences in the same sgRNA...increase stability and minimize misfolding and recombination during virus production needed to deliver CRISPR-Sirius...

...community-editable resource for storage and dissemination of information about FPs. FPbase.org FPbase...data to existing proteins when missing, or edit inaccuracies as they find them (anyone can register with ...community-editable database is free from the risk of inaccurate data submissions, so FPbase includes a versioning...

...these materials may not be available from their original sources. Figure 1: Biological repositories...availability of this strain from The Jackson Laboratory. Originally developed and characterized by Stanley Perlman...materials to be deposited into repositories, and financial support to maintain repositories’ capacity for...

...to break membranes of different organisms. And finally, we demonstrated that R bodies can be “tuned” with...F. R. Organization and expression of genetic determinants for synthesis and assembly of type 51 R bodies...trait: Absence of toxicity of R-body producing recombinant E. coli on paramecia. European Journal of Protistology...

... credit for small contributions. We strive to eliminate these two barriers, creating a community where...other scientists and to help in Crowdfight’s coordination work.  We also encourage you to file a request...Biomédica de Salamanca (IBSAL) and one of the coordinators at Crowdfight. She is currently based in Spain...