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  1. Clear and Accessible Writing

    Type
    Blog Post
    ...scientific paper, you may feel it’s overwhelming or challenging. So instead, let’s focus on practicing with a...dive into applying these four steps elsewhere, engaging in this practice, and perhaps modifying it as ...

  3. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...Even if you're currently a synthetic biologist engineering E. coli metabolism, maybe you’ll be able to apply...Optogenetics, Neuroscience, and the Future of Neuroengineering Primer on Optogenetics Resources at Addgene.org...

  6. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...Campus and the University of California, Berkeley engineered an AAV variant that efficiently infects neurons...recombinase-dependent vectors, this can cause challenges for the experimental design as these vectors ...

  7. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...typically their own genome, but this step can be engineered to be anything you want delivered to your target...and virus. Viral CRISPR-Cas9 tools have been engineered to introduce frameshift mutations in genes of...

  8. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...routine, open-source software projects that actively engage with the community are particularly successful....hardware, this process is often much more involved. Challenges such as the provision of an accessible supply...

  9. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...CRISPR-Cpf1 genome editing!  A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex...spCas9: ~4 kbsaCas9: ~3 kb ~3.9kb crRNA/gRNA length gRNA: ~100 nt crNA: ~42 nt Requires tracrRNA?...

  10. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...fragment, Fvs are often produced recombinantly and engineered to be joined by a linker peptide, resulting in...Tomisaki M, Kiuchi T, Takagi J, Watanabe N (2022) Engineered fast-dissociating antibody fragments for multiplexed...

  13. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...utilizes a gRNA which is photocaged and unable to engage a target sequence. Upon a brief light exposure ...Rosenberg, M., Hendel, A. Using Synthetically Engineered Guide RNAs to Enhance CRISPR Genome Editing Systems...

  14. To Each HIS Own

    Type
    Blog Post
    ...very pure sample. Because binding strength is proportional to the length of the tag, with longer tags you...

  15. All in a Twist: dsRNA

    Type
    Blog Post
    ...abundant in normal cells and are arise from a ssRNA engaging one strand of a dsDNA molecule; the 3-stranded..., but they also have a darker side. When ssRNA engages one strand of a dsDNA molecule, it does so at the...

  16. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...Institute website at alleninstitute.org. A classic challenge in cell biology is making sure that what we observe...of the protein gleaned from the literature and engagement with researchers who study the protein or structure...

  17. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...numbers of women participating in science and engineering. “Fix the Institutions” promotes gender equality...Gendered Innovations in Science, Health & Medicine, Engineering, and Environment—a project aimed at harnessing...

  20. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...Makerspace is composed of computer scientists, engineers, and biologists working together on a variety ... case.   Funding Makerspaces One of the main challenges when starting a new project at a makerspace is...
Showing: 521 - 540 of 791 results