Addgene

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Showing: 521 - 540 of 560 results

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CRISPR-mediated Plant Base Editors

Type

Blog Post

... other proteins. You can detect base editing as early as 48-72 hours post-transformation/transfection ...
Antibiotic Resistance: An Old Solution but a New Problem

Type

Blog Post

...are partially resistant. If you stop a treatment early, you may be un-wittingly selecting for less susceptible...
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

Type

Blog Post

...signal is significantly greater than miniTurbo at earlier time points but miniTurbo exhibits less labeling...
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

Type

Blog Post

...several split vector approaches developed in the early 2000’s which allow researchers to deliver oversized...
An Integrin Antibody Toolkit from IPI

Type

Blog Post

...intra- and extracellular environments. Found on nearly every cell in the human body, integrins are key...
Replacing Paper: Tips for Choosing an Electronic lab Notebook

Type

Blog Post

...Reproducibility Project: Cancer Biology have shown similarly disappointing rates of reproducibility. These ...
Transferable Skills Guide: Conflict Resolution

Type

Blog Post

...identify and address these types of harmful conflict early, because time frequently does not heal these types...
CRISPR 101: Homology Directed Repair

Type

Blog Post

...vertically at the orange arrows). If resolved dissimilarly (e.g. one junction is resolved on the crossing...
Deep Mutational Scanning with One Pot Saturation Mutagenesis

Type

Blog Post

...25167058. PubMed Central PMCID: PMC4412028. 5. Araya, Carlos L., and Douglas M. Fowler. "Deep mutational scanning...
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

Type

Blog Post

...peptides (Santana et al., 2020) and plants viruses (barley yellow striate mosaic virus (Gao et al., 2019) ...
Multiple Plasmids at a Low Price: Kits from Addgene

Type

Blog Post

...: 2388-2393. Huang, Shijie, Xuechen Zhu, and Charles E. Melançon III. "Detection and Quantification...
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Type

Blog Post

...systems. Both approaches may prove more successful early in the course of infection, when HIV-1 is limited...
Antibodies 101: The Basics of Western Blotting

Type

Blog Post

...could contain unintended cross-reactive proteins. Similarly, if you vary the experimental conditions from ...
Finding Your Perfect Job After University

Type

Blog Post

... a type of pediatric kidney cancer. This was particularly interesting as I had just completed my thesis...
Building Global Connections with the International Mentorship Program USA-EUROPE

Type

Blog Post

...President from the non-profit IMFAHE, Inc. She is particularly interested in breast cancer and metastasis as...
Viral Vectors 101: Optogenetic Tools

Type

Blog Post

...over when and where the receptor is activated. Similarly, PTLs are special, covalently linked molecules...
Technologies Enabled by NanoLuc® Luciferase

Type

Blog Post

...orange fluorescent protein, CyOFP1. CyOFP1 was molecularly evolved from mNeptune2 through 33 mutations and...
Plasmids 101: Dimers and Multimers

Type

Blog Post

...plasmid multimerization. Plasmid Multimerization Early studies on plasmid topology recorded plasmid multimerization...
Technique: Probe Phage Genomes for Host Binding Proteins

Type

Blog Post

... and a few proteases knocked out making them particularly useful for protein production), then dilute ...
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

Type

Blog Post

... or excise a gene that is located between them. Early recombinase-dependent vector designs used a lox-...

Showing: 521 - 540 of 560 results

Results per page:

CRISPR-mediated Plant Base Editors

Antibiotic Resistance: An Old Solution but a New Problem

Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

An Integrin Antibody Toolkit from IPI

Replacing Paper: Tips for Choosing an Electronic lab Notebook

Transferable Skills Guide: Conflict Resolution

CRISPR 101: Homology Directed Repair

Deep Mutational Scanning with One Pot Saturation Mutagenesis

CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

Multiple Plasmids at a Low Price: Kits from Addgene

Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Antibodies 101: The Basics of Western Blotting

Finding Your Perfect Job After University

Building Global Connections with the International Mentorship Program USA-EUROPE

Viral Vectors 101: Optogenetic Tools

Technologies Enabled by NanoLuc® Luciferase

Plasmids 101: Dimers and Multimers

Technique: Probe Phage Genomes for Host Binding Proteins

A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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