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Showing: 521 - 540 of 753 results

  1. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...favorite Cas enzyme, a guide RNA to your site of interest, and a donor DNA molecule containing your desired... these numbers may vary based on your locus of interest and experimental system.  Synthesizing ssODNs ...

  2. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...protein. SunTag has two components: 1) a protein of interest that’s tagged with 10-24 copies of the short epitope...portion of the receptors recognizes an antigen of interest while the intracellular T cell receptor portion...

  3. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...trainees at low wages will still be there without restructuring. Positive Points for Trainees - Taking Steps...of Research Website Additional Publications of Interest Can Be Found Here Resources at Addgene  Read...

  5. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...was in the lab, if you read a paper and saw an interesting construct you wished to use, you could either...Voltage Sensor  Ingaki and colleagues (8) were interested in using photoactivatable, optogenetic accuators...

  6. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...and repair pathways work downstream of Tet1 to restore the DNA after cytosine removal. Recently, Albert...Validated gRNAs to Target Your Genomic Locus of Interest Browse the CRISPR Collection ...

  8. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ... Zinc finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding...novel base editor proteins. She is particularly interested in combining the realms of art and science. Follow...

  9. Protocol - How to Design Primers

    Type
    Protocol
    ...complementary regions Note: If you will be including a restriction site at the 5’ end of your primer, note that ...the enzyme to cleave efficiently (e.g. GCGGCG-restriction site-your sequence). Protocol Video Watch the...

  11. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...selecting only bacteria containing the plasmids of interest (for example, fewer satellite colonies will grow...E. coli cells by heatshock with the plasmid of interest. See our transformation page for a detailed heatshock...

  12. General Transfection

    Type
    Protocol
    ...transfect mammalian cells with your plasmid of interest....Transfections allow for transient expression of a gene of interest in a target cell line and can be useful for short...

  13. Gibson Assembly Protocol

    Type
    Protocol
    ...extensively. Why Gibson Cloning? No need for specific restriction sites. Join almost any two fragments regardless...and screen for the correct plasmid product by restriction digest . Sequence the important regions of your...

  14. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...both antero- and retrograde labelling, synaptic restriction as well as controlled monosynaptic and polysynaptic....A., and Callaway, E.M. (2007). Monosynaptic Restriction of Transsynaptic Tracing from Single, Genetically...

  15. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ... You may also like... Plasmid Cloning by PCR Restriction Digest of Plasmid DNA DNA Ligation Background... DNA: Contains the portion of DNA that we are interested in amplifying for analysis and manipulation. ...

  16. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...generate stable cell lines expressing a gene of interest from an integrated lentiviral vector....generate stable cell lines expressing a gene of interest from an integrated lentiviral vector. Unlike the...

  17. Pouring LB Agar Plates

    Type
    Protocol
    ...your own LB/agar plates with your antibiotic of interest. Last Upload: September 14, 2016 Protocol Video...selecting only bacteria containing the plasmids of interest (for example, fewer satellite colonies will grow...
Showing: 521 - 540 of 753 results