...screens POSITIVE screen: Apply library Apply selection Most cells die or do not pass selection (in the case ...selection) In parallel, apply library Apply selection; most cells will survive Perform NGS on the surviving ...The selective pressure must be strong enough that most of the cells die, removing their plasmids from the.... Check out our blog posts featuring some of our most popular libraries! Genome-wide Screening Using CRISPR...

...Core Promoter The core promoter region is located most proximal to the start codon and contains the RNA...factors promote the initiation of transcription. The most 3' portion (closest to the gene's start codon) of...eukaryotes and archaea, however, contain this TATA box. Most prokaryotes contain a sequence thought to be functionally...contains transcription factor binding sites, but mostly contains regulatory elements. Eukaryotic Promoters...

...of viral integration have shown that lentiviruses most often integrate into actively transcribed genes,...p75 binding sites are enriched in gene bodies and mostly absent in promoters and intergenic regions, mirroring...promoter within the transfer plasmid must be modified. Most shRNA‐expressing lentiviral plasmids, such as pLKO...Broad tropism envelope protein used to psuedotype most lentiviral vectors. Packaging Gag in trans Precursor...

..., Addgenie Leila Haery has summed up some of the most important characteristics of retroviruses, lentiviruses...

...Addgene is poised to reach in the near future! We’re almost at:  100,000 different plasmids available from...

... will expand the repository in ways that will be most useful to you, the members of our community.   ...

...Addgene gave to me: MUCICAT to perform multicopy chromosomal integration.  On the tenth day of CRISPR, Addgene...

...CRISPR guide is a comprehensive resource that covers most things CRISPR - from nickases to base editing to...

...April Fool’s Day on the Addgene blog.  We’ve been mostly working remotely for the last year to give our ...

...YanevCellosaurus: A Cell Line Information DatabaseResearcher: Amos BairochViral Vectors 101: Systemic CapsidsResearchers...

...by Maki Naro   3)   “Bird and Moon” by Rosemary Mosco   4)   “Unearthed Comics” by Sara Zimmerman   5)...

...conference blog posts before you go!  Tips to Make the Most of a Scientific Conference Poster Session TipsHow...

... it regularly as people send me suggestions. The most useful parts of the Guide are probably the papers...

... suddenly. Please email [email protected] for the most-up-to-date information on shipping plasmids to your...

...GIPHY   We’ve been working hard to create the most informative and entertaining protocol and explainer...

...breakdown Introduction to Vini, Amy, and SITN The Most Difficult thing Vini, Amy, and Outreach Scientist...

... Troubleshooting Viral Transductions Getting the Most from Your Lentiviral Transduction Additional Resources...

... 3. Fossil Rock Anthem One of Tom's earliest and most popular videos. Describes fossil rocks and their...

...is a very commonly used CRISPR nuclease. Note: Most CRISPR plasmids from Addgene produce either a gRNA...

...the collection. You can find the full list here. Most of these plasmids are empty backbones, some of them...