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Showing: 521 - 540 of 812 results

  1. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...when the antibody is produced from a new set of animals), and wrong application (different experiments/...antibodies. A goldilocks antibody is highly specific (minimal binding to off-target molecules), reliable (wouldn...

  3. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...'t do it unless you can do it well" – even the smallest program. I don't mean have fancy food or a fantastic... put your panelists behind a table. It adds a formality and distance that prevents interaction with each...

  7. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...their versatile timer could be used in vitro, in mammalian cells, in C. elegans, and in Xenopus embryos. ... timers should therefore find use in studies of animal development where events like the patterning of...

  8. Transferable Skills: Negotiation

    Type
    Blog Post
    ...survey found that many researchers feel they lack formal training in a variety of transferable skills. At...easily work on this transferable skill without any formal training or coursework. First, you have to develop...

  9. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ... SNAPf-, CLIPf-, and CLAP-tagged proteins for mammalian cell expression. These plasmids will be useful...expresses all the components of the biosensor in optimal proportions from a vector backbone that allows ...

  10. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...dose (TCID50) (Zen et al., 2004).  To choose the optimal titering assay, you’ll need to understand how different...Levites, Y., … Golde, T. E. (2020). Utilizing minimally purified secreted rAAV for rapid and cost-effective...

  11. Of Myc and Men

    Type
    Blog Post
    ...five amino acids, meaning the tag could be quite small. However, substance P has an amide group added to...larger than the substance P peptide, but still quite small. However, unlike the substance P tag, they found...

  12. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...pCMMP-MCS-IRES-Puro IRES Retroviral pEF1a-IRES-Neo IRES Mammalian MSCV-IRES-Luciferase IRES Retroviral pWPI ...pWPI IRES Lentiviral AmCyan-P2A-mCherry P2A Mammalian pC5Kan-P2A P2A Insect pUltra P2A and T2A Lentiviral...

  13. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...can be used. The Fujii lab demonstrated this in mammalian cells by expressing dCas9 with an N-terminal 3xFLAG...expression of IGF2, a characteristic of many human malignancies. Additionally, the Tapscott Lab identified regulators...

  14. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...higher Tm give more efficient recombination. We normally use a Tm between 47-55 °C for the homologous region...be very helpful to confirm primer design.  Use minimal template DNA Use 1 ng of PCR template DNA to minimize...

  16. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...parties, and the company at large, by polling and informal communications we made a decision to try out a...the other hand if the journal club occurs with a small technical team (single lab or team), a deep technical...

  17. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...properly, it is unstable and may only work for a small number of qPCR runs before a new one is needed. ...and the droplet generation oil are moved through small channels to create a water-in-oil emulsion containing...

  19. CRISPR Antimicrobials

    Type
    Blog Post
    ...antimicrobial must reach essentially every cell or else small pools of antibiotic resistant cells could repopulate... systems used in both papers are analogous to mammalian viral vectors in that the vectors are replication-deficient...
Showing: 521 - 540 of 812 results