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  1. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...Campus and the University of California, Berkeley engineered an AAV variant that efficiently infects neurons...recombinase-dependent vectors, this can cause challenges for the experimental design as these vectors ...

  2. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...typically their own genome, but this step can be engineered to be anything you want delivered to your target...and virus. Viral CRISPR-Cas9 tools have been engineered to introduce frameshift mutations in genes of...

  3. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...routine, open-source software projects that actively engage with the community are particularly successful....hardware, this process is often much more involved. Challenges such as the provision of an accessible supply...

  4. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...CRISPR-Cpf1 genome editing!  A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex...spCas9: ~4 kbsaCas9: ~3 kb ~3.9kb crRNA/gRNA length gRNA: ~100 nt crNA: ~42 nt Requires tracrRNA?...

  6. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...fragment, Fvs are often produced recombinantly and engineered to be joined by a linker peptide, resulting in...Tomisaki M, Kiuchi T, Takagi J, Watanabe N (2022) Engineered fast-dissociating antibody fragments for multiplexed...

  7. To Each HIS Own

    Type
    Blog Post
    ...very pure sample. Because binding strength is proportional to the length of the tag, with longer tags you...

  8. All in a Twist: dsRNA

    Type
    Blog Post
    ...abundant in normal cells and are arise from a ssRNA engaging one strand of a dsDNA molecule; the 3-stranded..., but they also have a darker side. When ssRNA engages one strand of a dsDNA molecule, it does so at the...

  9. Giving gRNAs a Facelift - Synthetic and Beyond

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    Blog Post
    ...utilizes a gRNA which is photocaged and unable to engage a target sequence. Upon a brief light exposure ...Rosenberg, M., Hendel, A. Using Synthetically Engineered Guide RNAs to Enhance CRISPR Genome Editing Systems...

  10. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...numbers of women participating in science and engineering. “Fix the Institutions” promotes gender equality...Gendered Innovations in Science, Health & Medicine, Engineering, and Environment—a project aimed at harnessing...

  12. How to Prepare for an Industry Interview

    Type
    Blog Post
    ... time out of their day to talk with you, so be engaged with their conversation. Send thank you emails ...carefully. And even if you don’t have technical challenges, your interviewers might.  You’ll probably still...

  14. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...and deliver a variety of cargoes, genetically engineered neurotropic viruses emerged as powerful tools...Watabe-Uchida et al., 2012). They could also quantify the strength of the inputs by counting the monosynaptic inputs...

  17. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...safety features   When viruses were first being engineered as biological tools, thought was given on how...replication of virus and its infectivity in check. Engineering controls, safety systems that are built into ...

  18. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ... Eukaryota can also harbor these circular DNA passengers. Most natural plasmids encode beneficial traits...Gateway cloning. In addition, scientists have engineered entirely new types of plasmids without clear ...

  19. Hot Plasmids February 2024

    Type
    Blog Post
    ...here!   Zhang, Z., et al. (2023). Efficient engineering of human and mouse primary cells using peptide-assisted...Researchers in the Gradinaru lab have developed an engineered adeno-associated virus (AAV) vector named AAV.CAP-Mac...

  20. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...Institute website at alleninstitute.org. A classic challenge in cell biology is making sure that what we observe...of the protein gleaned from the literature and engagement with researchers who study the protein or structure...
Showing: 521 - 540 of 802 results