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  1. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...localization of the FP (fused to the protein of interest), thus tracking the localization of that biomolecule...at Albert Einstein College of Medicine. He is interested in everything related to gene expression, particularly...

  4. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...showing that AAV-mediated delivery of the RPE65 gene restores vision in the dog model of LCA (Lancelot, one ... again 3. Acland G. et al., 2001. Gene therapy restores vision in a canine model of childhood blindness...

  5. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...isolate bacteria containing your constructs of interest. But does it matter which antibiotic resistance... retained a functional copy of your plasmid of interest, preventing this issue. The genes and their antibiotics...

  6. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...biologists working with vectors are, in most cases, interested in transferring genes to mammalian cells (i.e... the viral RT protein (Faras et al., 1973). Interestingly, retroviruses are generally only “pseudodiploid...

  7. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...curve? A poor standard curve leads to over or underestimation of an unknown sample’s concentration. To determine...within the linear portion of the graph. It does restrict the dynamic range of your standard curve, so if...

  8. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...insert. Traditionally this has been done with restriction enzyme digest; however colony PCR can accomplish...Additional Resources on the Addgene Blog Learn to do Restriction Cloning Try SLIC cloning Learn about Gibson Assembly...

  9. Protocol - How to Design Primers

    Type
    Protocol
    ...complementary regions Note: If you will be including a restriction site at the 5’ end of your primer, note that ...the enzyme to cleave efficiently (e.g. GCGGCG-restriction site-your sequence). Protocol Video Watch the...

  10. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...envelope proteins from another virus to either restrict or broaden the host cell range (tropism). Pseudotyping..., Young JAT, Callaway EM (2007) Monosynaptic Restriction of Transsynaptic Tracing from Single, Genetically...

  12. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...scientists can make mutations throughout a gene of interest based on an organisms codon usage bias to increase...when do you want to codon optimize your gene of interest? Codon optimization has become extremely useful...

  13. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...understandable to scientists outside your field, and interesting. For example, “there is a significant need for...Campus of the Howard Hughes Medical Institute. His interests include the quality control of secretory proteins...

  14. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ... the ELN is reproducible, and only the highly restricted number of people who can access the ELN have ... the ELN arises here. Notwithstanding access restrictions based on permissions, however, in some cases...

  15. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...experiments with/for them. Though not the most prestigious role in a company, Technical Support is essential... open to experienced professionals with strong interest in the technical aspects of science and the drive...

  17. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ... answers to such questions, Addgene is deeply interested in pursuing them. We’ve recently revamped our...can help others as much as they've helped us.  Interested in providing feedback on AAVs at Addgene? Suggest...

  18. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...protein. SunTag has two components: 1) a protein of interest that’s tagged with 10-24 copies of the short epitope...portion of the receptors recognizes an antigen of interest while the intracellular T cell receptor portion...

  20. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...could be used to routinely deliver sequences of interest to cells with efficiencies of 1-40% after G418...designing homology arms with the modification of interest, ordering them from a gene synthesis company such...
Showing: 501 - 520 of 748 results