...localized fusion proteins and do not require dedicated plasmids or constructs. It should be noted that the photostability...Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire...

...Addgene.org Visit our cancer research plasmids and resources page  Find plasmids for your research Learn about...

...Resources Additional resources on the Addgene blog Plasmids 101: Aptamer FluorophoresAntibodies 101: scFVs...available from AddgeneBrowse our recombinant antibody plasmid collection References Grumezescu, A. M. (2018)....

...23953120. PubMed Central PMCID: PMC4096992. Find plasmids from this publication at Addgene. 2. Tang, Jonathan...2015): 1334-1341. PubMed PMID: 26258682. Find plasmids from this publication at Addgene. 3. Kirchhofer...

...recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid Will...sites to your recipient plasmid : You can modify the MCS of your recipient plasmid using Annealed-oligo ...material. We recommend 1.5-2μg of donor plasmid and 1μg of recipient plasmid. It is also critical that as much...re-circularization of the recipient plasmid. You should treat your digested recipient plasmid with a phosphatase prior...ligations for each plasmid you are trying to create, with varying ratios of recipient plasmid to insert. It...recipient plasmid + insert plate should have significantly more colonies than the recipient plasmid alone ... on your recipient plasmid alone plate, you can try ligating the recipient plasmid alone in the presence...

...for verifying plasmids like this is "plasmid fingerprinting", where you cut the plasmid into 3-8 pieces...used to verify your plasmid. The goal of a diagnostic digest is to cut your plasmid into specific sized...insert and the 5000bp backbone. Verifying Plasmid Identity by "Plasmid Fingerprinting" Often, it will be enough...for how to perform a diagnostic digest to verify plasmid size, verify insert orientation, and more. Protocols...Digest You may also like... Restriction Digest of Plasmid DNA Agarose Gel Electrophoresis Introduction Restriction...selectively move a specific piece of DNA from one plasmid to another. A diagnostic restriction enzyme digest...called restrictions sites. Often, the size of the plasmid insert and vector backbone are known and thus this...

... Protocols Plasmid Cloning by PCR Plasmid Cloning by PCR You may...recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid. Bonus...recipient plasmid. Remember to insert your DNA in the correct orientation in the recipient plasmid by viewing... 1μg of recipient plasmid. It is also critical that as much of the recipient plasmid as possible be cut...ligations for each plasmid you are trying to create, with varying ratios of recipient plasmid to insert. It...recipient plasmid + insert plate should have significantly more colonies than the recipient plasmid alone ... on your recipient plasmid alone plate, you can try ligating the recipient plasmid alone in the presence...

...   Addgene is sharing so much more than plasmids these days. via GIPHY   You can see for yourself...

...ready-to-use antibodies, viral vectors, and, of course, plasmids! Plus, we'll be giving away fun Addgene swag for...

... Addgene’s experience helping scientists share plasmids, especially those of the CRISPR variety (see slides...is in one of the top 10 countries in number of plasmid requests from Addgene. Addgene - Canton Nucleic...

...until now. Alice Ting’s lab has developed a new, plasmid-based biosensor to visualise changes in histone...Additional Resources at Addgene Find the Ting Lab plasmids at Addgene Browse More Fluorescent Protein Biosensors...

...Storage of Plasmids Recovering Plasmid DNA from Bacterial Culture Background Information Plasmids can carry...copy regardless of plasmid size. See the plasmid's info page to determine if your plasmid is high or low ...resistance gene on a plasmids allows researchers to easily isolate bacteria containing that plasmid from bacteria... isolate your plasmid DNA from the bacterial culture by following Isolating Your Plasmid DNA . Antibiotic... low copy plasmids? The copy number refers to the number of copies of an individual plasmid within a single...number plasmids should only need to be grown for 12-16 hr on average. Certain features of a plasmid may ...clonal) colonies of bacteria carrying a specific plasmid. However, a liquid culture is capable of supporting...

...Recovering Plasmid DNA from Bacterial Culture Recovering Plasmid DNA from Bacterial Culture You may also...concentrated plasmid DNA. This page will discuss the general procedure for purifying plasmid DNA from bacterial...perform plasmid purification without using a kit, you can find a protocol for kit-free plasmid mini-prep... the plasmid-containing supernatant. Add either ethanol or isopropanol to precipitate the plasmid DNA....high concentration of clean plasmid DNA. To improve the purity of plasmid DNA purified without a kit it...How to purify plasmid DNA from a bacterial culture, including protocols, tips, and FAQ Protocols... a new window) Promega sell kits for isolating plasmid DNA in quantities as low as a few micrograms to...

... also like... Plasmid Cloning by Restriction Enzyme Digest Restriction Digest of Plasmid DNA DNA Ligation...both storing and replicating plasmids. Because of this, nearly all plasmids (even those designed for mammalian...that will help to maintain the plasmid without rearrangement of the plasmid DNA. Additionally, specific ...tube of plasmid DNA and just need to transform bacteria so that you can grow up more of the plasmid) you ...efficient at taking up larger plasmids. If you need to transform large plasmids, it is a good idea to use ...Learn how to transform E. coli with your plasmid of interest. Protocols...introduced into a cell. Transformation of bacteria with plasmids is important not only for studies in bacteria ...

... Contribute your AAV data Just like Addgene’s plasmid repository, the AAV Data Hub is a community-driven...

...instructions and calculations on how to prepare a plasmid stock used to generate a standard curve, visit ...

...evolution. The Baker Lab has deposited multiple Top7 plasmids at Addgene, along with other tools like high resolution...

...stocks of their plasmids. This way, when you want to make more plasmid DNA, the plasmid will already be... Storage of Plasmids Creating Bacterial Glycerol Stocks for Long-term Storage of Plasmids You may also...important for long-term storage of plasmids. Although you can store your plasmid DNA at -20°C, many labs also...also like... Bacterial Transformation Recovering Plasmid DNA from Bacterial Culture Background Information... and growth temperature, can be found on your plasmid's information page. The next day you will be able...able to start an overnight culture for plasmid DNA prep the following day. Tips and FAQ The optimal concentration... glycerol stock the same time you prepare your plasmid DNA. In the morning, when you retrieve your liquid...

...vectors Career development for scientists A new plasmid tool your lab has deposited If you’d like to write...

...Agarose Method Plasmids 101: Mammalian Vectors Resources on Addgene.org Find mammalian plasmids for your ...