...plasmids from the same group should not be co-transformed, so if you need two plasmids for an experiment... same incompatibility group should not be co-transformed. +Actual copy number varies. See below for ...stocks, agar stabs, or liquid cultures. Freshly transformed cells also give a higher plasmid yield that colonies...SB, Furman JL, Xiao H, Schultz PG. A versatile platform for single- and multiple-unnatural amino acid ...

...Careers has developed a web-based, career-planning platform called the Individual Development Plan (IDP) and...professions Google potential careers to gather more information Follow people in academia, industry, and government...this is a valuable time to network Attend informational interviews to gain insight into the daily routine...much-needed break from your lab bench.  Want further information about shaping your career to your values, interests...

...around the poster (Reshef et al., 2020).The Twitter format meant that the posters could be viewed publicly...virtually means getting to know the app or virtual platform and making sure you stay connected. If possible...Networking can be more difficult in the virtual format than in-person. You might miss out on the short... adapt and take advantage of the new conference format. Additional resources on the Addgene blog Read...

...organism Yeast are single cell, eukaryotic organisms formally classified as fungi. They reproduce by either ...biotech and pharmaceutical companies is K. phaffii (formerly known as pichia). What is this new-fangled yeast...essential for sequestering certain enzymes and performing specific oxidative reactions. They are found ...predominantly uses non-homologous end joining (NHEJ) to perform DNA double strand break repair instead of homologous...

...category of enzyme, or name to discover all the information about that restriction enzyme or share it via... polymerase used. Overall, while much of the information in the app is restricted to the NEB inventory...very simple app that packs important biological information, amino acid abbreviations, and structures into... Blog Learn How to Keep a Lab Notebook for Bioinformatic Analyses Use Apps to Share Your Lab Protocols...

...however, can give you this information (more on this below). Plating the transformation on ampicillin plates... our blue-white screening blog post for more information.   Figure 1: Result of a blue-white screen...Sometimes common  primers can't provide all of the information you need, so you'll want to design a custom primer...

...improving access to useful research materials and information. Addgene has been successful in helping thousands...Addgene providing plasmids in a ready-to-use viral format. Having identified this need in the community,...The viral vector project team develops methods, performs extensive quality control, generates  educational... and Finance Teams continue to facilitate the formation of partnerships to expand the service and figure...

...cutting-edge research projects in synthetic biology and transforms them into modules that students and teachers ...Murray. Murray’s lab uses cellular extracts to perform transcription and translation in vitro, and the... mentors, and provides a nationally recognized platform in March to exhibit projects at any stage of completion...runoff from winter icing.BioBuilderClub is the only informal synthetic biology club of its kind, running from...

...extracellular surface of the GPCR. This results in a conformational change in the GPCR causing the activation of... in the nucleus. The Roth lab showed that this platform accurately identified 120 known GPCR targets demonstrating...post on GPCR signaling using TRUPATH for more information on this system! GPCR-APEX: Interested in tracking...either activate or inhibit neurons. You can find information more about chemogenetics and a concise table ...

...companies across most industries tend to hire their former interns to fill entry-level positions right out...grammatical errors, sentence structures, and overall format (fonts, font size, legibility, and more). Tips ...just as you would for an in-person interview. Formulate and practice a set of common questions with a ...technology, background, and lighting for a smooth performance. Most importantly, make certain that everyone...

...backbone, transformations require electroporation. References & Further Reading: For more information about...genome-wide library to activate all 23,430 known coding isoforms from the RefSeq database. The key to developing...crystal structure. “I thought maybe we could use information from the structure to guide and improve activator...

...proteins. The dTAG molecule induces ternary complex formation between the FKBP12F36V-fusion and the ubiquitin...Chemical Biology additionally provides detailed information on cloning procedures used to construct the deposited...dTAG-13 due its immediate action, potent ligand performance, great depth of degradation, absolute selectivity...FKBP12F36V-tagged proteins using our lentiviral expression platform, to assess selective degradation of FKBP12F36V-BRD4...

...longer term, this screening platform may help us to create high-performance sensors to enable multiplexed...mutants for further enrichment. Tian notes that this platform can be used to engineer many types of biosensors...encoded voltage indicator which had improved performance in live mouse brains. Currently, she is using...

... forces scientists to use invasive measures to perform in vivo optogenetic experiments. Intracranial injections... this library were designed using structural information about parental channelrhodopsins to create recombinant...correlated with the model’s predictions.  Three top performers, named ChRger1, ChRger2, and ChRger3, were then...minimally-invasive optogenetics While ChRgers performed well in a tissue culture dish, that doesn’t guarantee...

.... CombiGEM-CRISPR: The marriage of two simple platforms CRISPR has revolutionized how we decode the genome...regulation in biological systems. The CombiGEM platform provides a means to create barcoded gRNA libraries...library sequences via oligo synthesis using the format indicated below and pool-clone them into the pAWp28...Wong et al., 2016). With CombiGEM and CRISPR platforms now being integrated, we look forward to the realization...

...then return information such as nutritional status. Within the animal kingdom, this information is relayed...datasets and reagents in the field. Additional information can be found in the Datasets and Reagents sections... The development of the transient transfection platform for assaying transcriptional activity, for example...

...transitioned between different lab groups without having a formal interview process. Industry interviews, however...general questions about job responsibilities, performance evaluations, professional development, and company...ask the hiring manager or recruiter for this information. I found it useful to write my list of questions...the hiring manager or recruiter what presentation format they require. Frequently they will ask you to present...

...updates. Panel discussions are becoming a ubiquitous format for events and sessions in scientific conferences...good panel discussion is a well choreographed performance with room for fortuitous improvisation. You want... have the correct date, location and parking information and remind them about the event as it approaches... put your panelists behind a table. It adds a formality and distance that prevents interaction with each...

... need to deliver the Cas protein altogether. Platforming AAV Interest in interrogating and mapping the...glioblastoma, and generate programmable screening platforms for mouse tissues. Your AAVancement could be next...highly scalable cell type-specificin vivoscreening platform. bioRxiv (Cold Spring Harbor Laboratory). https...CRISPR Delivery Genome-wide Screening Using CRISPR Performing In Vivo CRISPR Screens Using the FITS Approach...

...important to understand that these platforms, or pieces of a platform, that are now available exist within...’s only the promises and associated sequence information. Addgene has partnered with the BioBricks Foundation... good ways of converting typically invisible information into actionable signals. Let's say I wanted to...