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  3. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ... Complementary to the released strand, 127bp in length, With 36bp on the PAM-distal side of the break ...al. "Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery....

  4. RUBY-Red Siliques

    Type
    Blog Post
    ... positive plants, but screening these can be challenging and requires specialized equipment. Reporters...Additional resources on the Addgene blog  CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9 Tips for Arabidopsis...

  5. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...metagenome datasets from hypersaline samples, were engineered and adapted for RNAi experiments for mammalian...mammalian cell lines. The Turner Lab Human messenger-RBP sgRNA Library targets human mRNA binding proteins with...

  7. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...other media outlets (AAAS Mass Media Science & Engineering Fellows Program). Science journalism programs...the Advancement of Science. She is enjoys broad engagement through science communication with a variety ...

  8. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...scientists and technical people) is linked to engagement and access to growth opportunities, not salary...zone when you lose track of time because you are engrossed in a task. Things seem to fall into place frequently...

  9. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...comments section below! Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.       Resources...

  12. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...distance.  From front to back: David Gordon, Jeff Guo, Fengbo Zhou, Paddy O’Leary (UCSF). Our approach is ...to Margaret Soucheray, Jeff Guo, Paddy O’Leary, Fengbo Zhou, Helene Foussard, and Dave Gordon) have been...

  13. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...from the MIT Sloan School of Management. Professor Feng Zhang’s original 2013 gene editing paper on CRISPR...References 1. Cong, Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." Science 339.6121 (...

  14. Plasmids 101: Environmental Plasmids

    Type
    Blog Post
    ...circular, extra-chromosomal, double-stranded DNA passengers. For example, a variety of high-copy number yeast...systems have been modified for use in genome engineering, including the Cre-lox system from the P1 phage...

  15. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...the Wyss Institute for Biologically Inspired Engineering and member of the Addgene Advisory board, on ...the Wyss Institute for Biologically Inspired Engineering and the Zhang and Doench/Root labs from the Broad...

  16. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...lines from becoming the new HeLa cells?), genome engineering and stem cells, and the difficulty of sharing...into a pig. In stark contrast to recent genome engineering technologies, stem cell techniques are neither...

  17. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...starting cell culture work. 3. NEB Tools by New England BioLabs In my lab we do a lot of genetic construction...experiments. Not all of our lab enzymes are made by New England BioLabs, but the NEB Tools app comes in handy when...

  18. Clear and Accessible Writing

    Type
    Blog Post
    ...scientific paper, you may feel it’s overwhelming or challenging. So instead, let’s focus on practicing with a...dive into applying these four steps elsewhere, engaging in this practice, and perhaps modifying it as ...

  20. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...Campus and the University of California, Berkeley engineered an AAV variant that efficiently infects neurons...recombinase-dependent vectors, this can cause challenges for the experimental design as these vectors ...
Showing: 501 - 520 of 791 results