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Showing: 501 - 520 of 812 results

  1. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ... replication, repairing mismatched basepairs or small indels that occur during DNA synthesis, and protomoting...preceded by GA or followed by TC) would be cut as normal. If not taken into account, this blocked site could...

  4. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ... possibly because in vitro conditions did not optimally support growth of diverse species. These results...Carlotta, et al. "Metagenomic engineering of the mammalian gut microbiome in situ." Nature methods 16.2 (...

  6. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...problem is, when it happens to other people, it’s normal and quickly forgotten, and when it happens to you...noises were just last week!  Nerves It’s completely normal to be anxious, nervous, and/or apprehensive. I ...

  7. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...resulting in two independent Fab fragments with small tails of the hinge region. Size: ~55 kDa.  Fab -...handful of advantages over full antibodies. Their smaller size allows them to get into samples more efficiently...

  8. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...becoming available to the community. This is no small task, however, at a repository that consistently...supplements), suggested sequencing primers, or mammalian selection markers. You don’t need a fancy database...

  10. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...specific, targeted DNA modifications in transgenic animals, embryonic stem cells, and/or tissue-specific cell...6276558. 2. Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage ...

  11. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...transitioned between different lab groups without having a formal interview process. Industry interviews, however...structured. I met with several individual and/or small groups, usually starting with the hiring manager...

  15. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...endothelial cell-pericyte, neuron-astrocyte, and cancer-stromal cell pairs.   Figure 2: Schematic of the... fluorescent proteins (FPs) fused to the AID* (minimal 44 amino acids auxin inducible degron sequence)...

  17. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...persistent immunological reconstitution with a normally functioning T cell repertoire (1). In this example...icosahedral, non-enveloped virus that carries a small, approximately 4.7 kb single stranded genome. AAV...

  18. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...publish brief novel findings. These results are normally one figure and can be negative and/or even replicated...develop computational methods to better understand animal development (Kyoda et al., 2012).  C. elegans plasmid...

  19. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...you. Once you input a sequence, SplitSet finds optimal fusion sites to split it into multiple fragments...antibiotic screening for selection, failed under normal cycling protocols, and required a 48-hour incubation...

  20. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...environmental plasmids, are all around us! A plasmid is a small piece of DNA that can replicate independently from... genetic parasites. Cryptic plasmids tend to be small and poorly studied, but some researchers have suggested...
Showing: 501 - 520 of 812 results