...well suited for larger transgenes. Unfortunately it has limited tropism in the retina, primarily infecting...Naldini,L., 2015. Gene Therapy returns to centre stage. Nature Vol 526, p.351. PubMed PMID: 26469046. 6...

...transduced by each serotype. Working at Addgene has shown me this was far from an isolated case. Our ...help each other by sharing their data. So take advantage of it and get your data - negative AND positive...

...experiments or a laundry list of molecules to screen has unclear utility. You cannot predict what will happen...Growth of new livers in culture could resolve this shortage." What is the knowledge gap you plan to address...

...two flanking repeats. In cells where recombination has not occured, methanol induces the production of the...that retain the ccdB gene die off and a higher percentage of transformants contain the ccdB-less construct...

...There are many moving parts to our software, which has grown with the company. It would be easy to assign...changing needs of the teams at Addgene. Another advantage of Scrum is that it provides transparency to everyone...

...the bench waiting for you, prep for research work has to be done by the researchers themselves.  Be ...rotation students, joining a new lab at any career stage, learning a new technique while visiting a lab, ...

...any immunogenicity of the AAV vector Once delivery has been optimized, it’s important to ascertain how long... Tabebordbar et al. Long et al. show that the percentage of dystrophin-expressing muscle cells increases...delivery must be optimized to: 1. Reach a high percentage of muscle cells throughout the body, especially...

...Looking forward Over the past twenty years, Addgene has made huge strides in increasing the accessibility...temperature-sensitive materials, we noticed a large percentage of these shipments arrived in India at room temperature...

...Bassik lab created a human knockout library that has 10 gRNAs per gene and targets all ~20,500 protein-coding...genome-wide screens.   Figure 1: Summary of the advantages of the new approach to genome-wide CRISPR screen...

...re-cutting the target sequence once the desired edit has been introduced. We found that blocking mutations...introduction of an intended mutation while taking advantage of the improved efficiency provided by blocking...

...effects, you can consider using a version of Cas9 that has been specifically engineered for reduced off target...sequence and other regions of the genome. The main advantage of NGS is that it allows you to simultaneously...

...few commonly used fluorophores. Each fluorophore has a defined emission maximum. Note, you can also use...itself, like laser type and power, acquisition voltage, the concentration of the fluorophore, and whether...bd-spectrum-viewer.   But what is the real-life advantage of a brighter fluorophore? Strong brightness makes...

...ever thanks to the virtual world that the pandemic has foisted upon us. Action Items:  A simple place to...an account and start engaging with the #scipol hashtag. Often there will be posts about virtual meet ups...

.../9R] ZIP3 Mouse Mouse IgG2a 206600 Anti-S-tag [N56/9R] S-tag Bovine Mouse IgG2a 206601 Anti-TrpC4 [N77...collection . Protocols The UC Davis/NIH NeuroMab Facility has several protocols available that may be helpful for... Human Mouse IgG2a 114476 Anti-Synaptotagmin-3 [N278/19R] Synaptotagmin-3 Mouse Mouse IgG2a 114477 Anti-TRPML3... Human Mouse IgG2a 114482 Anti-Synaptotagmin-12 [N277/7R] Synaptotagmin-12 Mouse Mouse IgG2a 114483 Anti-GluA1...) Rat Mouse IgG2a 114558 Anti-Synaptotagmin-10 [N269/73R] Synaptotagmin-10 Mouse Mouse IgG2a 114559 Anti-Ataxin... Mouse Mouse IgG2a 177526 Anti-Synaptotagmin-7 [N275/14R] Synaptotagmin-7 Mouse Mouse IgG2a 177527 Anti-Lgi1... Human Mouse IgG2a 188216 Anti-Synaptotagmin-6 [N270/47R] Synaptotagmin-6 Mouse Mouse IgG2a 188217 Anti-CASK...