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  1. Modulate the Activity of 17 Signaling Pathways with One Kit!

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    Blog Post
    ...cancers are treated with drugs designed to hit them right where it hurts, the effects are often remarkable...that confer resistance to drugs,” in a rapid, straightforward, and scalable manner, Wood said. Thankfully...

  2. From our Table to Yours: An Inside Look at Lunch at Addgene

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    Blog Post
    ...Right from the start, when we founded Addgene nearly 10 years ago, lunch felt like a family meal. My ... we have a few unique lunchtime traditions that might be fun to incorporate into your own lab. Iron Chef-style...

  3. X-CHIME: Context Dependent Germline Knockout in Immune Cells

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    Blog Post
    ...systems enable gene knockout in immune cells in a high-throughput manner, but these systems have not been...also includes pXPR_071, a plasmid that leads to higher titer lentivirus preparations than the first generation...

  6. London Calling - Addgene’s New Europe Office in London

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    Blog Post
    ...collaborators was extremely helpful, but with hindsight I wished I had gotten them from Addgene. I really...really struggled to get those plasmids with the right information and I believe that a lot of European ...

  8. Antibodies 101: Affinity Tags

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    Blog Post
    ...proteins from E. coli is generally more straightforward and of a higher purity than mammalian purifications...

  10. New Viral Vectors - Fall 2024

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    Blog Post
    ...monkeys it actually targets neurons, and has 45-fold higher expression levels compared to AAV9. You can now...AAV-CAG-GFP packaged in AAV9-X1.1!  We also have eight new AAV-PHP.eB vectors from the Jonathan Ting lab...

  11. Fluorescent Proteins 101: Fluorescent Biosensors

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    Blog Post
    ...signal. Reporter signals may be electrochemical or light-based, with luminescent and fluorescent reporters...transfer between the fluorescent proteins. FRET is highly amenable to biosensor creation, and custom FRET...

  12. Tips for Improving Your Next Manuscript

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    Blog Post
    ...leaders in the field of microbiology with multiple high-profile publications. My classmates consisted of...scientific manuscript is not prose; it is to be straight to the point. 3. Engage the reader Scientists ...

  15. The Stingy Scientist: How the Baby Gel Box Was Born

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    Blog Post
    ... I settled on using glass slides. With just the right amount of agarose, I could pour a very thin gel ...surface tension.  It's a bit tricky to pour the right amount so the agarose doesn’t pour over the sides...

  16. CRISPR/Cas9 FAQs Answered!

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    Blog Post
    ...that wildtype Cas9 with optimized chimeric gRNA has high efficiency but has been shown to have off-target...prefers a 'G' at the transcription start site to have high expression, so adding this G could help with expression...

  17. Choosing a Good Mentor for Scientists

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    Blog Post
    ...period. While there are many aspects to choosing the right labs (see my webinar (updated link from 2020) on...Mentoring" Don’t ignore warning signs If lab members, neighbors or alumni give you information about the PI that...

  21. Antibodies 101: Epitope Tags

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    Blog Post
    ..., is species compatible, and doesn’t come with a high background can be a huge challenge. Epitope tags...amino acids. FLAG tag has been used with moderate to high success rates across all applications. You can find...

  23. Using Addgene's New Viral Service for Your Research

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    Blog Post
    ...for similarly controlling neuronal activity with light will be available soon. We’re also distributing ...controls to determine if your AAVs are getting to the right cells using your particular delivery technique and...

  26. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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    Blog Post
    ...can't be too hard to apply. These papers suggest it might work well enough to consider as a therapeutic.” ...handle on how cleavage sites are repaired.” Those insights will be critical for scientists’ ability to influence...

  28. Networking Follow-up: I Met a Person. Now What?

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    Blog Post
    ...out of the incubator because I’d had a baby that night, all he said was “OK” and hung up on me). I went... 25 years since our last contact, but we were delighted to be reconnected.  Science can be a small community...

  29. 25 Work From Home Tips for Scientists

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    Blog Post
    ...great way to take a mental break, while meditating right before bed will help you prepare for sleep. At Addgene... Home. It’s normal and okay to feel overwhelmed right now, but hopefully these tips are helpful to you...
Showing: 751 - 780 of 986 results