...for verifying plasmids like this is "plasmid fingerprinting", where you cut the plasmid into 3-8 pieces...used to verify your plasmid. The goal of a diagnostic digest is to cut your plasmid into specific sized...insert and the 5000bp backbone. Verifying Plasmid Identity by "Plasmid Fingerprinting" Often, it will be enough...for how to perform a diagnostic digest to verify plasmid size, verify insert orientation, and more. Protocols...Digest You may also like... Restriction Digest of Plasmid DNA Agarose Gel Electrophoresis Introduction Restriction...selectively move a specific piece of DNA from one plasmid to another. A diagnostic restriction enzyme digest...called restrictions sites. Often, the size of the plasmid insert and vector backbone are known and thus this...