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Showing: 481 - 500 of 560 results

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Plasmids 101: Multicistronic Vectors

Type

Blog Post

...the same cassette is sometimes advantageous, particularly when only a portion of the plasmid is packaged...
Tips for Using BLAST to Verify Plasmids

Type

Blog Post

...COBALT for aligning multiple protein sequences, particularly for comparing different isoforms. In addition...
Of Myc and Men

Type

Blog Post

...c-Myc gene (Evan, et al. 1985). Myc was one of the earliest identified oncogenes and was a target of intense...
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

Type

Blog Post

...immunology PhD at Harvard University. He has had nearly a decade of experience working with Drosophila ...
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

Type

Blog Post

...PMCID: PMC3074624. 5. Bindels, Daphne S., et al. "mScarlet: a bright monomeric red fluorescent protein for...
Plasmids 101: Knockout/Knock-In Plasmids

Type

Blog Post

...researchers often use the Cre/Lox system described earlier. In this case, you design your targeting vector...
Google Forums Round Up: First Impressions of NgAgo

Type

Blog Post

...plasmid has been requested by labs around the globe nearly 400 times. Researchers have been excitedly testing...
Plasmids 101: Optimizing Plasmid Yields

Type

Blog Post

... might need to use multiple techniques for a particularly stubborn prep. Check the bacterial strain Increase...
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

Type

Blog Post

...increasing the signal from the second FP. Several early FRET biosensors were made with CFP and YFP, cyan...
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

Type

Blog Post

...many efforts in open science, Endy was recognized earlier this year by the White House as a Champion of Change...
Great Results Start with Great Standard Curves

Type

Blog Post

...consistent dilution factors, change pipette tips regularly, and mix samples very well. Use duplicates or...
Antibodies 101: Producing Recombinant Antibodies

Type

Blog Post

..., Zhan C, Malm M, Field R, Turner R, Sellick C, Varley P, Rockberg J, Chotteau V. Small-scale bioreactor...
DNA Purification Without a Kit

Type

Blog Post

...extraction method described in Kim and Morrison is nearly identical to that of commercially available kits...
Droplet Digital PCR for AAV Quantitation

Type

Blog Post

...droplet separation (Witte et al., 2016). This is particularly important if your amplicon is longer than 150...
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

Type

Blog Post

...journal articles. From paper to plasmid Authors regularly indicate that their plasmids are available from...
Prime Editing: Adding Precision and Flexibility to CRISPR Editing

Type

Blog Post

...world. This new approach, published in Nature earlier this week, is called prime editing. It’s a “search-and-replace...
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

Type

Blog Post

...cargos must be flanked by the L and R ends - thus, scarless insertions are not currently possible, unlike ...
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

Type

Blog Post

...References 1. Buchholz, Ursula J., Stefan Finke, and Karl-Klaus Conzelmann. "Generation of bovine respiratory...
A Career in Grants: How To Become a Grant Professional

Type

Blog Post

...particular area, e.g. education, poverty, environment, early childhood development, or community development?...
AAVs CREATed for Gene Delivery to the CNS and PNS

Type

Blog Post

...difficult it is to trace individual cell’s axons, particularly the two green neurons near the arrows. When ...

Showing: 481 - 500 of 560 results

Results per page:

Plasmids 101: Multicistronic Vectors

Tips for Using BLAST to Verify Plasmids

Of Myc and Men

Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

Plasmids 101: Knockout/Knock-In Plasmids

Google Forums Round Up: First Impressions of NgAgo

Plasmids 101: Optimizing Plasmid Yields

When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

Great Results Start with Great Standard Curves

Antibodies 101: Producing Recombinant Antibodies

DNA Purification Without a Kit

Droplet Digital PCR for AAV Quantitation

Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

Prime Editing: Adding Precision and Flexibility to CRISPR Editing

INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

A Career in Grants: How To Become a Grant Professional

AAVs CREATed for Gene Delivery to the CNS and PNS

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