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  2. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...from Golden Gate Cloning, in which a type IIS restriction enzyme and a ligase are used to assemble multiple...described with “it’s complicated.” Applying MoClo Interested in using MoClo? You don’t have to start from ...

  4. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...noise and ensure you are measuring the events-of-interest. Sensors suitable for ER measurements include:...scientists to focus on specific cell populations of interest. Many of the calcium sensors described are also...

  10. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...scientists can control expression of their genes of interest. While recombinases are powerful gene expression...cells to identify cells exhibiting properties of interest. However, FACS is unable to spatially and temporally...

  12. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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    Blog Post
    ...outskirts of Guangzhouon west of beautiful Mao Fengshan Forest Park. As a national science and technology park...surprising” small RNAs can be and posited that “we underestimate living things.” He further cautioned the distinction...

  13. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...in-situ hybridization, methylation-sensitive restriction enzyme digestion, DNA adenine methyltransferase...construct specifically to the histone protein of interest (in the figure above, H3 at K9 or K27). Joined...

  14. The Materials Science of Optogenetics Experiments

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    Blog Post
    ... are all wired up and expressing your opsin of interest, it’s time to get to the fun stuff: the experiments...Jeanne Kreek at the Rockefeller University.  His interests include the role of learning and memory in opioid...

  15. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...selected to preferentially target your cell type of interest. Viral pseudotyping is also a powerful tool that... optimize them for targeting your cell type of interest. For in vivo imaging applications, the ability...

  16. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...fusion protein containing VP2C and a protein of interest (POI). The VP2C fusion protein anchors to VP1,...into a compartment encapsulating the protein of interest (Figure 3).   Figure 3: Schematic representation...

  18. Plasmids 101: Optimizing Plasmid Yields

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    Blog Post
    ...DNA to verify your plasmid via sequencing or restriction digest. Chances are, you’ll even have DNA left...Transformation Learn How to Perform a Diagnostic Restriction Digest Additional Resources Sigma-Aldrich Introduction...

  19. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...localization of the FP (fused to the protein of interest), thus tracking the localization of that biomolecule...at Albert Einstein College of Medicine. He is interested in everything related to gene expression, particularly...

  20. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...showing that AAV-mediated delivery of the RPE65 gene restores vision in the dog model of LCA (Lancelot, one ... again 3. Acland G. et al., 2001. Gene therapy restores vision in a canine model of childhood blindness...
Showing: 481 - 500 of 753 results