...validating your new clones, try these tips to make the most of your time and sequence! You might also enjoy ...GenBank, EMBL, DDBJ, and PDB sequences and may be the most comprehensive for searching. Timesaving Tip #1:...

...Z., Li, L., Liu, J., Huang, Z., Xie, H., Liu, D., Mo, H., Zhou, R., Lang, B., Meng, B., Weng, H., & Hu...inversions with two gRNA sites on the same chromosome. Most multiplex systems enable researchers to target...simplicity and adaptability, CRISPR rapidly became the most popular genome engineering approach. Figure 1: Overview...ease of generating gRNAs makes CRISPR one of the most scalable genome editing technologies, making CRISPR...mutations (see: Validate genetic modification ). In most cases, NHEJ gives rise to small indels in the target...homology elsewhere in the genome. Realistically, most gRNA targeting sequences will have additional sites...specificity. SpCas9 (from Streptococcus pyogenes ) is the most popular Cas endonuclease, with many modified versions...

... Types’ I selected ‘Lentiviral’ which eliminates most of the plasmids designed for other uses. I could...inspiration for our updated mascot, it’s also a convenient most requested filter. Applying a second filter, this...

...’t we use it? This last section was arguably the most important, and it was a little worrisome for me ...basically impossible for textbooks to keep up. Even the most ambitious teachers, who work hard maintaining a ...

...fluorescence microscopy, is another option. However, most SRM techniques also require specialized instruments...requires fixed samples. This limitation may be the both most obvious and least flexible one. Tissue expansion...

...post-translational modifications that bacterial and most yeast cells cannot. Your choice of production system... the amount of protein you need to produce.  The most common yeast strain used for recombinant antibody...

...-contianing “ligand” that binds to the tag. Like most good ideas in biological imaging, the initial breakthrough...proteins makes them the go-to choice for many (if not most) fluorescence microscopy experiments. However, when...

...’s your favorite part about working at Addgene?” Most Addgenies reply with an enthusiastic, “Our company... atmosphere...

...easy for scientists to re-synthesize DNA with the most optimized sequence. Unfortunately, it is not always...compared to others. Image from Hiss et al., 2017. Most commonly, scientists codon optimize genes from eukaryotic...

...communicate your story with only the most salient details. Make the most of your limited speaking time. Beyond...

...A similar genetic code is used by most organisms on Earth, but different organisms have different preferences...Nonsense! I should just choose the codon with the most abundant pool of charged tRNAs in my host organism...

... the excision of the phage from the bacterial chromosome and the regeneration of attP and attB sites. ...inhibits the growth of CcdB sensitive E. coli strains, most colonies should contain the desired, recombined ...

...Antibody here!   Anderson, Z., Zhu, H., Riedel, T., Moshinsky, D., Meijers, R. (2024). Western Blot for Anti-V5...signal and can be used in light-sensitive systems. Most excitingly, assembly of the split luciferase is ...

...field of research], and have excellent [list the most important skills for the position]. I am enthusiastic... does not need to be a literary masterpiece. For most jobs, the writing quality required will be close...

...transgenic zebrafish are well established (Felker and Mosimann, 2016). One common and robust strategy uses a ... Central PMCID: PMC6247916. Felker, A., and C. Mosimann. "Contemporary zebrafish transgenesis with Tol2...

...several sgRNA’s at the same time, and choose the most effective ones to increase your chances of gene ...the pronuclei were fusing, you may have several mosaic mice. To figure out what alleles are present in...

...included two reference tables below listing some of the most common bacterial and mammalian promoters. These ...mRNA Strong plant promoter from the Cauliflower Mosaic Virus Constitutive  Active in dicots, less active...

... from chromosomal DNA. Although they can be found in archaea and eukaryotes, they play the most significant...conclusions that genes encode proteins and reside on chromosomes, which are composed of DNA. These findings culminated...order to isolate a particular gene from human chromosomal DNA, it would be necessary to isolate a sequence...coined the term plasmid, in reference to any extrachromosomal heritable determinant. Plasmids are fragments...relationship with the host cell. Like the bacterial chromosomal DNA, plasmid DNA is replicated upon cell division...DNA from one context to another, such as from a chromosome to a plasmid. These tools are essential to the...recombinant DNA Molecular Cloning Plasmids that are used most commonly in the field of recombinant DNA technology...

... Since its appearance, SARS-CoV-2 has spread to almost every part of the world manifesting as a full-fledged...Containing the spread of this virus has become an utmost priority for countries around the world and to ...

...scientists using aptamers might know this best, as most fluorescent aptamers are named for a fruit or vegetable...2014). While 10 derivatives of TO exist, Mango is most frequently used with TO1-biotin or TO3-biotin (the...