Skip to main content
Addgene
Showing: 481 - 500 of 812 results

  1. What the HEK?

    Type
    Blog Post
    ... is present in this line, making it genetically female. HEKs are generally cultured as an adherent monolayer...blog Five Popular Model Organisms 10 Tips for Mammalian Cell Culture Adenoviral Vector Production and...

  2. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ... from the those used to replicate the host's chromosomal DNA, but they still rely on the host machinery...streaked bacteria have higher copy numbers – for optimal results always pick a single colony and do not ...

  3. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...valuable for CRISPR/Cas9 stem cell editing. The small molecule NSDB and Na+ hypertonicity are key elements... Introduction of macromolecules into cultured mammalian cells by osmotic lysis of pinocytic vesicles. ...

  4. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...yourself to be as productive as you would be in a normal situation. For some other resources on working...Science, or Resources to Engage Kids at Home. It’s normal and okay to feel overwhelmed right now, but hopefully...

  5. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...examining relationships between microbes and insects, mammals, and plants, among others. I particularly enjoyed...doesn’t love a book series that features a young female protagonist, some clever magic, and a satisfying...

  6. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...generations of iGluSnFR had limitations: they were not optimal for experiments requiring high signal to noise ...fusion to the PDGFR transmembrane domain in the mammalian expression pMinDisplay vector. GPI contains a ...

  8. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...and has extensive experience targeting RNA with small molecules. Other members’ projects range from hacking...Ph.D. grad student at Boston University studying mammalian immunology, and in his free time, he is pursuing...

  9. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...plasmids in the library are differently sized, smaller plasmids may be amplified preferentially, skewing...Brunello library is shown in Figure 2. You can use a small PCR product because the plasmids in the library ...

  10. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...handling recommendations Not all O-rings function optimally at -80°C. For samples that are shipped on dry ...changes in our solutions are transient and return to normal once the tube is opened and equilibrium with ambient...

  11. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...scientists to express a gene of interest in a cell or animal model. AAV plasmids contain several important elements...enzymes cut at known sites within the ITR. However, small deletions and point mutations will go undetected...

  12. Hot Plasmids - January 2023

    Type
    Blog Post
    ...alternatives - RNAi and Cas13. CRISPR-Csm doesn’t degrade proximal RNAs nor does it have high levels of off-target...changes of non-target genes and RNA-seq of a nuclear (MALAT1) and cytoplasmic (CKB1) RNA target. Adapted from...

  13. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...recently released our serotype testing AAV, which are small (20 ul) samples of EGFP-expressing AAV packaged ...resting fluorescence and can be used for imaging small neuronal processes (dendrites and axons). jGCaMP7c...

  14. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ... Assembly method from New England Biolabs® has minimal upfront requirements and allows you to expediently...generation, green chemistry and understanding the minimal genome. Many of these techniques require more complex...

  15. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...PAM-distal side of the break and 91bp on the PAM-proximal side, consistently supported higher frequencies...attained without any of the error-prone repair that normally accompanies Cas9 cutting. We still don’t know ...

  16. Components of CRISPR/Cas9

    Type
    Blog Post
    ...or closely related Cpf1) to cut the genome and a small RNA (gRNA) to guide this nuclease to a user-defined...used for CRISPR-mediated genome editing, these two small RNAs have been condensed into one RNA sequence known...

  18. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...H2B (histone)-GFP reporter into the genome of mammalian cells.  Figure 1: The SRIRACCHA reporter...create GEmCherry2. The Denham lab made several other small changes creating 2 other versions but these did ...

  19. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...reverse tetracycline-controlled transactivator) is normally inactive and cannot bind the tetracycline response...Similarly, the various eukaryotic cell types (mammalian, yeast, plants, etc.) require unique promoters...

  20. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ... attain cell-specific expression in transgenic animals, where cre expression is restricted to a particular... which can then be exclusively activated by the small molecule clozapine N-oxide (CNO) (Krashes et al....
Showing: 481 - 500 of 812 results