...since bacteria won’t divide immediately after it has been heat-shocked or electrically zapped. As a result...cleavage in human cells. Mutagenesis, 20(2), 111–114. https://doi.org/10.1093/mutage/gei016I Resources on ...

...Noninfectious ARK mouse embryo cell lines in which each cell has the capacity to be activated to produce infectious...namely that they can cause cancer via insertional mutagenesis (which HIV does not do). Furthermore, molecular...useful for viral reproduction, and for causing contagious infection; however this would not be great for...

...technology potential enabled by CRISPR-transposons has yet to be fully realized. Many thanks to our guest...applications - as mutagenesis tools for genetic studies (e.g. using mariner), as a method of tagmentation for next-gen...

...expression is sufficient. Lentiviral vector technology has come a long way since the 1990s, both in terms of... popular and useful viral vectors in the lab. Advantages of lentivirus include a large genetic capacity...research is still in the pre-clinical/early clinical stages. Researchers are also working to make lentiviral...

...caveat that phages are very diverse, and each phage has a narrow bacterial host range. To further potential...antibiotic resistance. Advantages of CRISPR over traditional antibiotics The potential advantages of CRISPR over...

...experiment check out Addgene’s protocol page, which has both protocols and videos for techniques in basic...transferred is commonly a plasmid and can infer genetic advantages such as antibiotic resistance. Bacterial...

...repair work. The key is T4 DNA polymerase, which has 3′→5′ exonuclease activity. T4’s polymerase activity...structures at the ends of fragments. The major advantage of SLIC over Gibson assembly is cost, as T4 polymerase...

...several reasons why doing something without a kit has advantages: less plastic waste, less expense, and less...

...-optical psMEK1 + ERK KTR-mRuby2 system has several advantages over existing methods. First, it avoids...

... the RNA backbone to increase stability.  Nature has evolved ways to prevent degradation of important ...less off-target events. While both have similar advantages, bridged nucleic acids with N-methyl substitutions...

...can’t it’s an easy search. Writing this tutorial has shown me that I’m still in the dark ages with respect... at 1 paper/week will be useful. Check out the hashtag #365papers on Twitter for inspiration and accountability...

...assistance in the grant application process, she has faith in you! Additionally, keep in mind that universities...of current methodologies. This section sets the stage for why your funded proposal will address these ...

...Department of Genetics of Harvard Medical School, has a mission of raising awareness and starting conversations...who are interested in such careers should take advantage of their academic environment and attend seminars...

...availability of both wild-type and mutant loxP sites has allowed scientists to leverage this system in new...site-specific recombination. The FLEx switch takes advantage of the orientation specificity of the site-specific...

...Addgene have worked hard to ensure that the service has grown steadily, helped by a partnership with the ...available from Addgene as of 2023. A large percentage of viral vector preps contain biosensors, optogenetic...Figure 2). Biosensors, which include calcium and voltage sensors, are the broadest category of viral vector...

...Product Marketing Manager at sciNote. Today, every lab has its own habits and approaches to record keeping. ...about ELN costs and limited budgets, “a large percentage of survey respondents indicated that cost was...effort and give the lab a serious competitive advantage.  Many thanks to our guest blogger, Tea Pavlek...

...inch wireless monitor. Wireless video technology has gotten very impressive, but due to costs and availability...leisure. For virtual B-Roll (that’s the secondary footage you often see playing over the voice-over in videos...: If you’ve ever watched any behind the scenes footage in the last several years, you can usually see ...

...fluorescent molecules, including fluorescent proteins, has led to the alternative acronym Fluorescence Resonance... et al., 2008), and monitor neuronal activity (voltage sensors), to name a few.   Find Many FRET-Based...

...division. Recently Seán got the entrepreneurial bug and has set up an ELISA kit company on whose website you ... Strategize, choose calls that suit your career stage / research area, and focus on producing a good quality...

...know this?” Over the past fifteen years, the CBM has developed a student-centered SMART Team modeling ... of proteins using 3D printing technology. The tagline for our company is “…where molecules become real...