Addgene

We narrowed to 802 results for: Eng

Showing: 481 - 500 of 802 results

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Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

Type

Blog Post

...widely available imaging platform: ultrasound. Challenges of in vivo imaging with existing bacterial reporters...genes (ARGs) Acoustic Reporter Genes (ARGs) are engineered gene clusters that encode proteins that form ...
Bringing Sustainable Practices to the Lab: Recycling

Type

Blog Post

...for laboratory waste symposium, sponsored by New England Biolabs and Labconscious. I was eager to learn ...Labconscious. If you’re inspired by this post then I challenge you to take to action! These days, it’s important...
An Addgene Co-op: The Intersection of Fun, Friendship, and Work

Type

Blog Post

... both knowledgeable in their field and eager to engage with the work of others. Having an environment ...at once. I was able to do something that was challenging while helping us share our resources better with...
Embracing Serendipity: A Crucial Element in the PhD Journey

Type

Blog Post

...your primary focus lies within a biological lab. Engaging in such seminars exposes you to discussions beyond...release and mental freedom. Practical approaches Engaging in mindful meditation, taking field trips, and...
Antibodies 101: Fc Effector Functions

Type

Blog Post

...macrophages bind IgG antibodies. The macrophages then engulf and digest the pathogen the antibodies are bound...are intriguing from a therapeutic standpoint. Engineered monoclonal antibodies that can drive specific...
CRISPR 101: Drug discovery and CRISPR

Type

Blog Post

...is more on the horizon. Cas enzymes have been engineered to have many unique capabilities which has advanced...Resources CRISPR History and Development for Genome Engineering ...
Management for Scientists: Managing vs. Leading

Type

Blog Post

...motivating their doers by ensuring they are as engaged as possible. To learn more about being an effective... and Richard Badham Leadership Insights for Engineers, Scientists and Computer Professionals as Leaders...
Management for Scientists: What Makes a Good Manager Anyway?

Type

Blog Post

... the successes of the team. A strong sense of engagement in the work Behind-the-scenes support from their...identify the work style of each team member) Need challenging work, opportunities to pursue and problems to...
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

Type

Blog Post

...using sgRNAiBAR. Image from Zhu et al., 2019. Wensheng Wei's lab at Peking University has developed a...recombination sites in the construct to different lengths, the density of cells that are labeled can be altered...
Identifying Sequence Elements with SnapGene's Feature Database

Type

Blog Post

...controls available when needed. SnapGene has been engineered to be easy and enjoyable to use. Development ...annotation process. Many of the best ideas come from engaging with users of the software. We welcome suggestions...
Science Careers: Unruly Interests Feed Many Paths

Type

Blog Post

...other media outlets (AAAS Mass Media Science & Engineering Fellows Program). Science journalism programs...the Advancement of Science. She is enjoys broad engagement through science communication with a variety ...
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

Type

Blog Post

...are single domain antibody fragments typically engineered from heavy-chain only antibodies found in camelids...catalytically inactive dCas9 by fusing it to an engineered peroxidase, (APEX) that labels proximal proteins...
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

Type

Blog Post

...Because mNeonGreen wavelengths lie between the green and yellow fluorescent protein wavelengths, it can be imaged...
Tetbow: Bright Multicolor Labeling for Neuronal Tracing

Type

Blog Post

...23817127. PubMed Central PMCID: PMC3713494. Ke, Meng-Tsen, et al. "Super-resolution mapping of neuronal...reports14.11 (2016): 2718-2732. PubMed PMID: 26972009. Ke, Meng-Tsen, and Takeshi Imai. "Optical clearing and index...
Fluorescent Biosensors for Measuring Autophagic Flux

Type

Blog Post

...Regardless of excitation wavelength, Keima emits at 620 nm. The ratio of signal strength excited at 586 nm to...
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

Type

Blog Post

...screening platform, Oliver Griesbeck’s lab set out to engineer a new FP. Their aim - to red-shift the boundaries...expansion of the genome editing toolkit. However, a challenge in optimizing base editing technologies across...
RUBY-Red Siliques

Type

Blog Post

... positive plants, but screening these can be challenging and requires specialized equipment. Reporters...Additional resources on the Addgene blog CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9 Tips for Arabidopsis...
Hot Plasmids and Viral Preps - September 2021

Type

Blog Post

...metagenome datasets from hypersaline samples, were engineered and adapted for RNAi experiments for mammalian...mammalian cell lines. The Turner Lab Human messenger-RBP sgRNA Library targets human mRNA binding proteins with...
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

Type

Blog Post

...spring requiring an overnight hike in. Navigating challenges: collecting and processing environmental samples...,241 amino acids long, has optimal sgRNA spacer length of 22-25 nts, and is thermostable between 31-100...
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

Type

Blog Post

...staining can be a challenge in the best of cases. But it can be particularly challenging when your only ...

Showing: 481 - 500 of 802 results

Results per page:

Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

Bringing Sustainable Practices to the Lab: Recycling

An Addgene Co-op: The Intersection of Fun, Friendship, and Work

Embracing Serendipity: A Crucial Element in the PhD Journey

Antibodies 101: Fc Effector Functions

CRISPR 101: Drug discovery and CRISPR

Management for Scientists: Managing vs. Leading

Management for Scientists: What Makes a Good Manager Anyway?

Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

Identifying Sequence Elements with SnapGene's Feature Database

Science Careers: Unruly Interests Feed Many Paths

Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

Tetbow: Bright Multicolor Labeling for Neuronal Tracing

Fluorescent Biosensors for Measuring Autophagic Flux

Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

RUBY-Red Siliques

Hot Plasmids and Viral Preps - September 2021

Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

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