...miRNA efficiency on a target sequence. The gene of interest is cloned into a plasmid downstream of luciferase...transcript containing luciferase and the gene of interest permits translation of functional luciferase, ...

...tissue types, recombinases allow you to further restrict gene expression to areas in which the recombinase...expression patterns, thus allowing you to further restrict gene activation to cells expressing both Cre and...

...and then unfollow those who do not capture your interest during that week.” You can also find Dr. Knoepfler...consistently engage with others about your topic(s) of interest. Hopefully through these links, I’ve: 1) convinced...

...open-reading frame and separated from the protein of interest by a 2A self-cleaving peptide (Goedhart et al,...correlation between the fluorescent protein of interest and the reference protein (Goedhart et al, 2011...

...about the various posters I visited. If you’re interested in neuroscience but are afraid you don’t have...virus for you. Neuroscientists are particularly interested in using AAVs because they provide a highly versatile...

...partnership with high school teachers to boost interest and understanding around the engineering of biology...by emailing [email protected]. Of particular interest this fall:  The BioBuilderClub BioBuilderClub ...

...RNA (gRNA) used to target the genomic locus of interest. As these off-target effects can be genome-wide...can allow for targeting of your genomic loci of interest that may not be accessible by wild-type Cas9, ...

...the intent is to make a knockout in your gene of interest, as it is prone to generating indel errors. Indel...bloggers!   David Wyatt is a graduate student interested in determining how the structure of broken ends...

...every project, time, attention, and/or length restraints mean you will have to pick and choose what concepts...feedback in a variety of ways, like having them restate or summarize the concepts you’re covering. Don'...

... allows synthetic biologists to use Type IIS restriction sites to easily assemble multiple DNA fragments...dimerize, bringing each terminus of Cre together to restore Cre activity. As soon as red light or PCB is no...

... approach to simultaneously clone two genes-of-interest (GOI) into two independent expression cassettes...pUC57-L3L2) that offer the advantage of using restriction digest cloning to introduce the GOIs into the...

...considered "natural tumor suppressors." A growing interest in the cancer microenvironment Ever since Stephen...Viva" in Aveiro, Portugal. She is particularly interested in science journalism and writing about biomedical...

...along with an antibody targeting the protein of interest led to rapid, targeted degradation that is dependent...independently control expression of your genes of interest. These transcription factors consist of the GAL4...

...s start with the paper itself: Ma et al. were interested in correcting an autosomal dominant mutation ... vs repair from the maternal MYBPC3 allele. Interestingly, they found that the embryos underwent repair...

...growth factors or serum to stimulate a pathway of interest, but there can be a lot of static since other ...inserting pdDronpa domains into loops of a protein of interest. If you plan to try this, it’s important to check...

...assay with your chosen FP in your cell type of interest before starting your experiments.  Selecting a...this fluorescent protein to tag your protein of interest will be problem-free. In figure 1 we provide an...

...When we developed this tool, there were so many interesting questions that we could apply [it to].” Using...Pharmacology at Novartis Oncology and is particularly interested in applying what we have learned about tumor ...

...dithiothreitol, DTT), Invitrogen NP0009 PageRuler Plus Prestained protein ladder, Invitrogen 26619 Pipette tips...the hot plate to 100 °C. Thaw IgG standard and prestained protein ladder on ice. Reagent Preparation Prepare... that the chamber is sealed. Load 5 µL of the prestained protein ladder to the appropriate well. Pro-Tip...

.... One popular approach is to fuse a protein of interest to each part of a split fluorescent protein (FP...the behavior of the split FP in your system of interest. Remember that the FP fragments themselves can...

...considerations: Design your gRNA to overlap the CpG of interest. Having the CpG directly inside the gRNA sequence...but if this is not possible due to gRNA design restrictions, anything within 5 base pairs should work well...