...of the ABE8s ranged from 1.5x higher for positions A5-A7 to 3.2x higher for positions A3-A4 and A8-A10...synthetic library of TadA sequences to create the eighth generation of adenine base editors, called ABE8... in variants named “ABE8.x”: “8” stands for the eighth round of ABE evolution and the “x” is the placeholder...editing frequencies. For applications that require high DNA editing specificity, for example for potential...

...installment of the Early Career Researcher Toolbox will highlight four tools for finding journal articles related...summary of the topic and a chronological list of highly cited papers that contain your search term. When...of the page, as well as the number of citations highly influenced by this paper. Influence is measured...number of publications that heavily cite, or are highly influenced by the author. You can also see who ...

...possible by the use of our high-throughput DNA isolation process that yields high-quality isolated DNA samples... on our ability to provide requesting scientists high quality data! Addgene's new in-house NGS process...be familiar with our efforts to ensure access to high quality, ready-to-use viral preps and pooled libraries...Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F (2017) Genome-scale CRISPR-Cas9...

...these high levels of viral replication are toxic. Second, infecting subsequently higher and higher order...transmission occur, spreading the virus through higher and higher order neural connections. While this transsynaptic...

...Updated Apr 6, 2021. If you’re doing high throughput work, it’s likely that you come into the lab wishing...transformation One protocol that has been automated is high throughput transformation. With our ever growing...the deck of the Biomek. The FXP id also able to do high throughput DNA preps and other molecular work if... through this process. With enough agar and the right program, the liquid handler can fill 400 vials in...hand. How can you start automating your lab? You might not be doing enough transformations, minipreps, ...

...expression and degradation. These workshops not only highlighted new tools but also included time for questions...presented at the C. elegans meeting. Here I’ll highlight a few toolkits that we have available at Addgene..., and Erik M. Jorgensen. "SapTrap, a toolkit for high-throughput CRISPR/Cas9 gene modification in Caenorhabditis...

...above, or other. High or Low Copy Choose from: high or low copy. Choose “high copy” if a sufficient...standard cloning strain DH5α. For plasmids with highly repetitive sequences (prone to recombination in...Example of the deposit spreadsheet showing the first eight columns and a pop-up instruction box for the “Purpose...

...of unionization continues to be a polarizing one. High-profile universities remain outspoken in their opposition...FLSA changes, including finding money in already tight lab budgets and even simply being able to categorize...salary perspective, while this is a move in the right direction, the increase notably still does not meet...Workshop 2016 At past symposia, FoR worked to highlight some of the problems facing postdocs and graduate...

...chains and two small light chains that stick out to form the “arms” holding the highly variable regions that...membrane proteins that may unfold due to camelid high body temperature (37.2 - 39.2°C). The Seeger lab...References Geertsma ER (2013) FX Cloning: A Versatile High-Throughput Cloning System for Characterization of...resources on the Addgene blog Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification...

..., and so on and so forth. This also serves as a light technical control - an RQ value of 1.5 means you...quencher to quantify specific amplicons.  Sybr has high background noise, due to the large amount of dsDNA...DNA and the length of the amplicon and it has a higher rate of false positives (Colborn, 2008; Harridan...number of factors will determine which approach is right for your experiment. You’ll have to consider:  ...analysis of copy number variants based on real-time LightCycler PCR. Curr Protoc Hum Genet. 2014;80:7.21.1-7.21...

...Addgene we've set out to fill this gap by both highlighting that researchers do learn MANY transferable ...the lab and by offering advice on areas where you might need some help. Today in our transferable skills...you will have a much better chance of creating a high-performing team - which is every manager’s dream... a weekly basis! The silence and maybe even the slight awkwardness can spur people to speak up. Once aware...

...quality. To make sure our AAV vectors are of the highest quality, we undertake a rigorous quality control...vectors, and helper plasmids (Chadeuf et al., 2005, Wright et al., 2011). In fact, the majority of hits come...samples from 0.1-20% and discovered that, with a high enough sequencing depth, we could detect contamination...with exquisite detail (Lecomte et al., 2015). This higher level of quality control is recommended but not...Acids 4:e260 . https://doi.org/10.1038/mtna.2015.32 Wright JF, Zelenaia O (2011) Vector Characterization Methods...

... vivo CRISPR screens. AAV vectors can be made at high titers equal to those achieved with lentivirus but...less involved setup when working with mice and slightly less red tape for approval processes. Additionally... means you can customize your CRISPR screen to a high degree. This is particularly helpful when investigating...Breakefield, X. O., Maguire, C. A., & György, B. (2019). High levels of AAV vector integration into CRISPR-induced...screening by AAV episome-sequencing (CrAAVe-seq) is a highly scalable cell type-specificin vivoscreening platform...

...using a high-speed ultracentrifuge. During the spin, the virions will form a band due to their high density...nationwide where you can obtain excellent quality, high titer rAAV (including Addgene!), but you can also...terminal repeats (ITR). You should always have a high-quality plasmid standard and rAAV reference material...Zen, Z., Espinoza, Y., Bleu, T., Sommer, J. M., & Wright, J. F. (2004). Infectious titer assay for adeno-associated...

... backbone might be more prone to recombination, while a library with a large backbone might be more difficult...to ensure the highest possible transformation efficiency. Using Stbl4 or other ultra-high efficiency cells...specifically calculated based on the size of and weight of your library plasmids. As long as you achieve...

...cells to grow and divide only at the right times and in the right places. Checks and balances: protecting...proteins naturally lack areas that are accessible, high-affinity binding targets for potential therapeutic...therapeutic agents. Nevertheless, a glimmer of light remains for this elusive target. Recent improvements in ...results and customize the small molecules for a higher affinity fit. Kevan Shokat’s group at the University...

...project she needs one of you to lead. At first you might think, “I’m not a leader,” because you don’t see...you. How is this new opportunity much different? Might you already have leadership skills that are just...and develop your nascent leadership skills. You might just find that you’re more of a leader than you ...External collaborators For further advice on highlighting your transferable skills in that big job interview...

...ancestry groups rather than self-reported race, it highlights the myriad issues researchers need to reflect...associated dependencies across ancestry groups (right).    Seeking an explanation  First, they looked...guides from being designed that target regions with high genomic variability.   Moving forward It took a ...Stephanie M. Fullerton et al. Getting genetic ancestry right for science and society." Science 376, (2022): 250...

...now, 2 decades later, is it finally back in the limelight. On December 19, 2017 the FDA approved Luxturna... brought all these key elements together at the right time and place. But it is the hard work and persistence...immunogenicity is critical when systemic delivery of high doses of vectors are needed. Additionally, AAVs ...ultra-rare ocular diseases are being benched due to the high cost of clinical development. As a result, Luk Vandenberghe...8. Trapani I and Auricchio A., 2018. Seeing the Light after 25 Years of Retinal Gene Therapy. Trends Mol...

...excluding Starbucks. Taking a nerdy perspective, you might denote your search as follows: (my_house + coffee...math illustrated. Intersect (left) and set minus (right). Enhancers are regions of DNA that demonstrate...enhancers, check out the Appendix. Conclusion: Shedding light on dark matter Precise knowledge of which stretches... courtesy of beyondthedish (left) and biobabel (right). People commonly think of DNA as a linear polymer...meters long. You twist and roll the string into a tight ball. Now the two ends of the string, instead of...