...’s a good thing, though! Viral delivery has several advantages over other mechanisms. For one, many viral...Cellular phenomena such as exocytosis, autophagy, voltage, and tension all have specific sensors designed...

... is easy, after all, who working in a laboratory has never faced an odd situation with experiments? Our...major challenge when we started was creating a LEGO stage that resembles a real laboratory. It only took one...

...In these instances, the cloning vector typically has two separate resistance cassettes and your gene of...Additionally, the presence of a plasmid is disadvantageous from the bacterium's perspective – a plasmid-containing...

..., another method to create point mutations, also has a strict editing window about 15 nt upstream of the...NGAG and NGCG and SaCas9 with an NNGRRN PAM by mutagenizing the PAM-interacting domain. However, these alternative...mutants with PAM flexibility. In this system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation...recognizing multiple PAMs should have a fitness advantage over proteins with single PAM recognition. ...cell population measured with NGS) are shown as percentages, with fold changes compared to SpCas9 in parentheses...

...The IgnaviCas9 protein is 1,241 amino acids long, has optimal sgRNA spacer length of 22-25 nts, and is ...Spring in Lower Geyser Basin. They used a mini-metagenomics method using microfluidic parallelization to...

...their advantage should they decide to produce virus on their own.Harvard researcher Connie Cepko has been...

...writing, etc. Also, it is unlikely that a mentor has the only relevant expertise and perspective for your...been doing something all wrong or that a reagent has not been convincingly demonstrated to work for your... be needed to achieve your intended next career stage and create a timeline for achieving these goals....

... exposure to increased intracellular calcium. It has been applied to mark active populations of neurons...using viral vectors! One of the most significant advantages of viral delivery is the potential for long-term...results in calcium assays. There’s another crucial advantage: tissue-specific targeting. By employing various...

...for aerosol contamination. As most centrifugation has to be done outside of a biosafety cabinet, you’ll... organizations or cities may require a higher percentage of the decontaminate to be used for BSL-2 waste...decontaminating liquid waste, remember that the percentage indicates the proportion of the decontaminate...

... Prize in Chemistry for his work. Since then PCR has become a standard and essential practice in molecular...generating mutated sequences for site-directed mutagenesis.  Quantitative polymerase chain reaction, qPCR...interest  Molecular Cloning Site-directed mutagenesis Molecular Diagnostics qPCR Quantify starting... PCR  Plasmids 101: Colony PCR Site Directed Mutagenesis by PCR Resources on Addgene.org Protocol: Polymerase...

... proteins, respectively. A plasmid's copy number has to do with the balance between positive and negative...for single- and multiple-unnatural amino acid mutagenesis in Escherichia coli. Biochemistry. 2013 Mar 12...

... interviews are 1:1 discussions with someone who has interesting experiences to share. The conversation...they can share on their resumes have a definite advantage in our process. We see candidates who have done...

...plasmid by sanger sequencing. DNA replication by PCR has error rates that range from roughly 1 per 500bp to...final Reverse Primer sequence of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Preparing the PCR product for...

...individual who is a local expert in that task (or has the bandwidth to become an expert) the end result...get more done. There are also a host of other advantages to practicing effective delegation skills: ...

...regions of the gene is also useful in case the gene has a weird mutation that you didn’t know about (which...and is a testament to how far genome engineering has come. For comparison, in 2007 it took about 3 months... the bands on your gel and get an idea of the percentage of cells in the population that have an edit....

...internal ribosomal entry site and mCherry. If a variant has wild-type levels of abundance, the eGFP will be stable...modeling, directed evolution, site-saturation mutagenesis, and medium-throughput screening, she increased...

...this meeting is to _________." Everyone in the room has likely just run in from some other commitment. An... what you need to accomplish to get to the next stage, and then adjourn. Define the tone of the meeting...

...replicate faster. For this reason, if your library has inserts of different sizes (such as a transcription... is less than that of electroporation, but an advantage is that chemical transformations are generally...Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled Library 80,962 1866 bp, plus...

.... This can be helpful as you're learning! Ashlyn has a research background in CD8+ T cells and cancer ..., unlike voltages, you can adjust compensation values later in your analysis, unlike voltages.  Figure...

...be used to determine how long a pool of FP timers has been expressed, either in whole cells or as a pool...knowledge of protein structure and saturation mutagenesis. mK-GO matures from green to red, and the FT ...absorption spectra). Subach et al’s directed mutagenesis also produced the three separate timers shown...