We narrowed to 893 results for: plasmid
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TypeBlog Post...module, which are also available as lentiviral plasmids if you want to make a stable cell line. This design...developing embryo is accessible for injection of plasmids and other materials. Figure 3: Optogenetic...Guide or browse our collections of optogenetics plasmids – and keep an eye out for future updates to the...on Addgene.org Optogenetics Guide Optogenetics Plasmids References Aljiboury, A. A., Ingram, E., Krishnan...
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Microbes: Look, Listen, and Tell
TypeBlog Post...Resource Page CRISPR plasmids designed for use in bacteria Deposit Your Microbiology Plasmids with Addgene ... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post...on the Addgene Blog Plasmids for endogenous gene tagging in human cells Plasmids 101: Protein Tags Fluorescent...from many pitfalls: they require construction of plasmid vectors and/or rely on cumbersome in vitro synthesis...directly purchased and there is no need to construct plasmid vectors or synthesize/purify IVT sgRNA in the lab...SapTrap Resources on Addgene.org Browse CRISPR plasmids Check out our CRISPR guide pages Find ready-to-use... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog Post... GFP marker (CatCH-GFP). The lab deposited AAV plasmids containing the active promoters at Addgene. Performance...of the retina. Find the AAV synthetic promoter plasmids! Comparing synthetic promoters performance in ...blog posts Resources at Addgene.org Find AAV plasmids Find ready-to-use AAV viral preps Browse all viral... -
Bringing Sustainable Practices to the Lab: Innovations
TypeBlog Post...other scientists save resources by sharing plasmids Find plasmids at Addgene ... -
Editor's Choice, August 2016
TypeBlog Post... can use PCR to make small sequence changes to plasmid DNA. This an oldie but a goodie, and in my personal...experience, is a quick way to generate many mutated plasmid constructs. The second post was contributed by ... -
Six Spooky Science Stories and Halloween at Addgene
TypeBlog Post...Plasmid pumpkins, team costumes, and spooky science stories. Yes, please! At Addgene, we love finding... contest trophy. We’ve had teams dressed up as plasmids, Blugene, and the Addgene ping pong table. This... -
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog Post... Visit Our Stem Cell Page Find Plasmids for Reprogramming Find Plasmids for Differentiation ...to a Nobel Prize for Dr. Yamanaka in 2012. Find plasmids for generating iPSCs iPSCs and ESCs closely resemble... -
Of Myc and Men
TypeBlog Post...Antibodies 101: Monoclonal Antibodies Plasmids 101: Peptide Tagging Plasmid-based Recombinant Antibody Resources... recombinant anti-c-Myc [9E10] at Addgene Find plasmids with the myc-tag ... -
Polymerase Chain Reaction Overview and Applications
TypeBlog Post...Digital PCR for AAV Quantification Plasmid cloning by PCR Plasmids 101: Colony PCR Site Directed Mutagenesis...amplicon can be used in molecular cloning to create a plasmid of interest, for detecting the presence of an insert... -
Typing CRISPR Systems
TypeBlog Post...recently that researchers began building optimal plasmids to harness the power of Class 1 systems. Class...Lastly, type IV systems are most often found on plasmids. The many unique features of IV-A and IV-B have...led to the hypotheses that they are involved in plasmid competition or can hijack machinery from other ...highly diverse and involved in competition between plasmids. Nucleic Acids Research, 48(4), 2000–2012. https... resources on Addgene.org CRISPR guide CRISPR plasmids CRISPR references ... -
Scientific Sharing in the Time of COVID-19: Databases and Resources
TypeBlog Post...researchers have been making new materials like plasmids available to scientists around the world. To support... Resources on Addgene.org Browse our COVID-19 plasmid collection ... -
The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place
TypeBlog Post...CRISPR guide Learn more about plasmid pooled libraries Find CRISPR plasmids for your research ... -
A Better Way to Get Customer Support: The Help Center
TypeBlog Post...Features on Our Plasmid Pages Resources at Addgene Visit the Help Center Find Plasmids for Your Research... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog Post....1111704108. Epub 2011 Aug 9. PubMed. Find the Plasmids @Addgene: Plasmids for KD, R, B2, and B3 recombinases from... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog Post...procedures. The video details how to transfect CRISPR plasmids into MEL cells by electroporation, sort your transfected... PubMed. More CRISPR Refereces: Browse CRISPR Plasmids CRISPR Information at Addgene More CRISPR-... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog Post...thanks for depositing your plasmids with Addgene. Howarth: We submit all our plasmids that may well be of use... -
The Stingy Scientist: How the Baby Gel Box Was Born
TypeBlog Post...was to make my own glass milk for purifying cut plasmid DNA from agar slices. Molecular biologists used...bromide, less stop buffer and miniscule amounts of plasmid sample. How satisfying? With practice, I got ... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog Post...Read our plasmids 101 blog series Resources on Addgene.org Browse our zebrafish CRISPR plasmid collection...collection Read our molecular biology reference Find plasmids for your research ... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...because it relies on a single BiFC pair. Find BiFC plasmids for your experiment at Addgene! How to Design ...straightforward in easy-to-transfect cell lines where plasmid dosage can be tightly controlled. This is also ...Resources on Addgene.org Find fluorescent protein plasmids and resources Visit our Molecular Biology Reference...