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  1. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...was to make my own glass milk for purifying cut plasmid DNA from agar slices.  Molecular biologists used...bromide, less stop buffer and miniscule amounts of plasmid sample.  How satisfying?  With practice, I got ...

  5. Light Sheet Fluorescence Microscopy

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    Blog Post
    ...compared to transgenic animals (17).  These viral plasmids are available through Addgene.  The combination...University of Miami Miller School of Medicine. Find plasmids from the Lee Lab here. Pantelis Tsoulfas is an...University of Miami Miller School of Medicine. Find plasmids from the Tsoulfas lab here.     References: H...

  6. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...for mammalian RNAi Browse all plasmids for RNAi Check out Addgene's plasmids and resources for plant biology...harvested instead of microscopic isolation. The plasmids required to conduct an APT-based RNAi experiment... target sequence, as well as essential control plasmids. Many thanks to our guest blogger Robert Orr...

  10. Year of the Camelid: Antibody Style

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    Blog Post
    ... 12–15 kDa Ease of cloning and expression from plasmids, due to only needing one open reading frame Ability...Membrane Proteins More resources from addgene.org  Plasmids encoding nanobodies at Addgene Sybody Generation...

  13. Using Addgene's New Viral Service for Your Research

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    Blog Post
    ...accelerate your research.   Why distribute virus? Plasmid distribution is booming, but, over the years, many...list of what’s available or suggest additional plasmids with the Viral Service Suggestion Form. Lentivirus...

  15. Overcoming the AAV Size Limitation for CRISPR Delivery

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    Blog Post
    ...capabilities of full-length SpCas9. This set of plasmids includes AAV-Cas9C-VPR for targeted gene activation...bacterial immune system cleaves bacteriophage and plasmid DNA. Nature 468:67–71 . https://doi.org/10.1038...Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids ...

  18. An Addgene Summer Internship

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    Blog Post
    ...something wrong. My capstone project was based on plasmid cloning and I, the researcher, got to choose what... we found with the correct bands was the right plasmid, so after discussion, we sent it for sequencing...

  19. Viral Vectors 101: Transductions

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    Blog Post
    ...typically transfected along with viral packaging plasmids into a viral production cell line, usually HEK...HEK-293 cells. When all of these viral plasmids are expressed within the production cell line, viral particles...
Showing: 441 - 460 of 883 results