... the then RNAi Platform, which has since evolved into the Genetic Perturbation Platform and encompasses...Associate Director of the Genetic Perturbation Platform at the Broad Institute and has worked with many...The model that the Broad was building that had Platforms with a capital ‘P’ focused on technology ... has...enjoys working on technology development under the Platform model at the Broad Institute because they are ...CRISPR Learn more about the Broad Institute and the Platform Model. ...

...open repository of plasmids and other necessary information that’s required to do research. Paratore: What...scientific community through Addgene’s sharing platform. Expanding further beyond the US and increasing...: The promise there is the ability share the information, making it available to everyone, that creates...breakthrough and innovation. With any kind of open platform, there is the potential for increased speed and...certified public accountant (CPA), certified information systems auditor (CISA), and certified health ...both the Internal Institute of Auditors and the Information Systems Audit and Control Association. Lastly...

...engineers regularly use the platform, why scientists are using the platform, and tips for getting the most...from Nature Jobs Blog While Twitter is a popular platform for scientists, it’s not the only tool in a scientist... Facebook. For each of the three social media platforms, there’s some statistics on what percent of scientists...research potential future employers and to arrange informational interviews. 4. “Tips for Scientists Using Social...given you tips for using various social media platforms, and 3) helped you fit social media into your ...

...ways scientists communicate with each other: from formal mechanisms like publishing a research article, ...or submitting patent or grant applications; to informal settings like lab meetings, Twitter, coffee chats...blogging and more. These each have different goals, formats, and processes, and they provide different amounts...one of the key routes for researchers to share information about the emerging SARS-CoV-2 virus quickly and...before, submitting the manuscript to a journal for formal peer review. Some organizations, including some...preprints are eventually published in journals after formal peer review, with the earlier attention and feedback...

...through the literature until you have all of the information you need. Reading primary research literature...written. By nature, scientific literature is information dense and since it continually builds on previous...The introduction should give you just enough information to allow you to understand the research question..., statistics, clarity in images etc)? Did they perform all of the appropriate controls? Refer back to ...Are there any red flags about how the work was performed that could influence the results? The peer-review...misinterpretations. After all, the science was performed, written and evaluated by humans. Keeping up with...

...intracellular parasites and natural vehicles for genetic information. Therefore they make excellent tools for genetic...same gene products for packaging, however, the isoforms of these proteins, as well as the viral LTRs differ...be combined with high-throughput sequencing to perform multiplexed analysis of cells pre-treated with ...Consortium, also known as the Genetic Perturbation Platform, to produce their shRNA library to support functional... with an shRNA sequence of your choice. More information and a detailed protocol on how to use the pLKO...www.genome-engineering.org for the most up-to-date information. Check our CRISPR guide and blog to learn more...

... to be recruited per targeted gene. Performance SunTag performs better than first generation activators...elongation, depending on which sequence is targeted. Performance dCas9-VP64 activation is generally thought of...additional activation domains (HS1 and p65). Performance When targeting single genes, SAM consistently...in tandem to recruit transcription factors. Performance Generally, VPR is found to have significantly... WA (2013) Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression...

...the glut of information, but it’s not in the best interest of any company to hide information about their... surprised to discover that the most crucial information about a product is printed right on the tube ...pages on website are another great source of information. At least at Addgene, the questions in our FAQ...the resources to recapitulate the experiments performed by our thousands of depositors. That means their...specifics. We can help you faster if we get as much information as possible about the issue. For Addgene support...

...found that many researchers feel that they lack formal training in a variety of transferable skills. At...stayed until he graduated. Years later I attended a formal training for new managers and I had a “Eureka” ... have a much better chance of creating a high-performing team - which is every manager’s dream. Learn ...often associate feedback with end-of-the-year performance review, but feedback should be given throughout...the year and in a timely manner. If someone’s performance or behavior is inadequate, you need to address... “bad” managers and I've learned from both. The former will empower you, keep you motivated and help you...

...cancer dependency database used to make this information accessible to the research community. Sean Misek... to guides in the Broad’s Genomic Perturbation Platform Avana library (used by the Cancer Dependency Map...of the dependencies detected. Given this new information, Misek notes that in theory, if the DepMap analysis...used CRISPR screens, they had also found the information they needed to begin correcting the issue. Working...and Adam Brown from Broad's Genetic Perturbation Platform to design a new library that is agnostic to ancestry... are working with Broad's Genetic Perturbation Platform to modify the CRISpick algorithm to prevent guides...

...challenge at hand and help resolve it through well-informed recommendations. Healthcare consulting firms that...to get a large amount of market research done to inform a go/no-go decision in a very short amount of time...pressure to maintain consistently high levels of performance, and occasionally being faced with “shifting ...crucial to how our clients’ products will end up performing in the market. Hence, as a PhD, an MD, or someone... successful therapeutics can be a great fit for former healthcare consultants. I have even seen consultants...especially helpful since they both provide up-to-date information about the industry, recruiting processes, and...

...Schematic of BeadScan platform for screening candidate biosensor variants for performance under two-photon ...high-throughput screen to select variants based on performance metrics specifically for two-photon microscopy...Near-infrared (NIR) fluorescent proteins promise better performance for in vivo use and deep-tissue imaging. Vladislav...concentrations of lactate. The microfluidic screening platform developed here is also a promising approach for...produce a temperature sensor that significantly outperforms previous designs. B-gTEMP is excitable under ...

...CSV file, anytime. The CSV is a rich source of information and has data on the date, material, requesting...requesting PI & organization, and country. This information can be of personal interest to the depositor, but...interesting to parse through, aggregation of this information can provide valuable insight to the most requested...why I turned to dashboards. Dashboards provide information in a concise and clear way that is accessible... the public sharing of aggregated data only, information on requesting PI(s) & organization(s) is not ...

...useful research materials and information. Many of our technology platforms like HaloTag® Fusion Protein...caught the attention of researchers wanting to perform whole-animal imaging, however NLuc’s emission peak...responsive protein binds to calcium it changes conformation and brings the two fluorescent proteins together...Many thanks to our guest blogger Kyle Hooper. A former Technical Services Scientist, Kyle Hooper, PhD,...Machleidt, T., et al. (2015) NanoBRET-A novel BRET platform for the analysis of protein-protein interactions...

...asked the individuals behind some of these new platforms what their motivations were for developing these...COVID-19 to researchers in other disciplines (bioinformatics, image analysis, AI…).  How to put the wider...Resources Canada is a database that provides information about reagents, funding and volunteer opportunities...this program is based in Canada, much of the information is applicable to researchers worldwide. “COVID...research in their field including datasets and platforms for discussing new developments, and more. As ...

...Emerging CRISPR/Cas9 editing technologies have transformed the palette of experiments possible in a wide...recombination repair pathway. Reagents required to perform pha-1 co-conversion are available through Addgene...ensuring the PAMs remain on the chromosome after DSB formation, was most efficient (Farboud et al., 2019). Using... C. elegans. The C. elegans work on small RNAs informed and drove work in countless other systems, whereas...CRISPR technology into C. elegans has been a transformative innovation for our community. Developments ... genomic locus (CRISPR-ChAP-MS) could further transform the range of experiments imaginable in C. elegans...

...found that many researchers feel that they lack formal training in a variety of transferable skills. At... of my colleagues, I can also orchestrate the formation of high-functioning-but-temporary teams that have...for guiding new deposits into the repository, performing quality control, and providing technical customer...goal of your project to launch a product? Make an informed decision about something? Fix a problem? Test ...specialize in order to deal with all the new information in the world, so a process or project or mission...on the other side of the world, communication platforms like Slack make teamwork easier. Trust Ultimately...

...is to know how the AAV vector you intend to use performs in your system. This includes a thorough testing...you time in the long run since not having this information could lead to waiting longer than necessary before... the supplier of your vector, to obtain this information.  Optimize titer for the DIO/FLEx vector Once...experiments requires a full understanding of vector performance in your system. It is very rare for a methods...known and unknown factors that affect vector performance in each and every system and cell type. Therefore... Novel Next-Generation Sequencing and Analysis Platform to Assess the Identity of Recombinant Adeno-Associated...

... or drug treatment can browse through screens performed in relevant cell lines or conditions. This increased...searching the term "breast cancer" returns 40 screens performed in 26 different breast cancer cell lines. Screenshot...without the need to analyze raw sequence data.  This format is inherently flexible and allows the broadest ... will be adaptable to a wide variety of screen formats and contexts and continue to improve the accessibility...joint effort by the BioGRID ORCS team. For more information and updates follow BioGRID and BioGRID ORCS on...

...solve problems is to work together and share information broadly. “Otherwise we will all have to wait ...is an Addgene depositor. KSQ uses a CRISPR-Cas9 platform to systematically test the function of all 20,...the targets that have the biggest potential to transform patients' lives. Previously Stegmeier was the ...also interested to see Addgene expand the sharing platform to other reagents in biology. Luk H. Vandenberghe...he interviewed Addgene co-founder Melina Fan and former Associate Director of Viral Vectors Karen Guerin...