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  1. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...Pk1] in a western blot assay. Image reused from Anderson et al., 2024 under CC BY license.    Find ...Find Anti-V5 [IPI-SV5-Pk1] Antibody here!   Anderson, Z., Zhu, H., Riedel, T., Moshinsky, D., Meijers, R...

  2. Hot Plasmids Spring 2024

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    Blog Post
    ...proteins, and modifications to the pegRNA. The Britt Adamson Lab recently used a CRISPRi screen to identify ...useful materials for research into Alzheimer’s, Parkinson’s, ALS, and Huntington’s Disease, including plasmids...

  3. Tips for Writing a Good Cover Letter

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    Blog Post
    ... Quick tips for writing a good cover letter Personalize your cover letter to the job and job requirements...Soriano-Carot in September 2016 and was updated by Rachel Leeson in May 2024.     Additional Resources on the Addgene...

  4. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...Langan, E., Siebert, S., Li, J., Greiner, D., & Anderson, D. (2015). Neutrophil Responses to Sterile Implant...  Rachel Leeson...

  5. To Codon Optimize or Not: That is the Question

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    Blog Post
    ...regulatory elements or use a non-canonical code (Gustafsson et al., 2004), such as Hoogsten base pairs and...Biol 13:30–34 . https://doi.org/10.1038/nsmb1031 Gustafsson C, Govindarajan S, Minshull J (2004) Codon bias...

  7. Important Considerations in Optogenetics Behavioral Experiments

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    Blog Post
    ...summarized in a short blog post, but some examples I’m personally interested in include: intracranial self-stimulation... to proceed with your behavioral experiments. Comparison of c-fos amongst animals that receive different...

  8. Plasmids 101: E. coli Strains for Protein Expression

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    Blog Post
    ...act on DNA, or are enzymatically active. For this reason, recombinant proteins are typically expressed in...culture from reaching the desired density within a reasonable time-frame. For these cases, some strains carry...

  9. Harnessing Bacterial Toxins for Allelic Exchange

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    Blog Post
    ...arguably the most widely and productively used  (Thomason et al., 2014). In recent years, CRISPR-Cas9 technology...reports 5 (2015): 15096. PubMed PMID: 28060411. Thomason, Lynn C., et al. "Recombineering: genetic engineering...

  11. The Fluorescent Vegetables in Aptamer Soup

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    Blog Post
    ...do aptamers! Corn was recently described in 2017 (Song et al., 2017). Corn naturally dimerizes, and this...PMID: 28747322. PubMed Central PMCID: PMC5602116. Song, Wenjiao, et al. "Imaging RNA polymerase III transcription...

  12. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    Blog Post
    ...is also being realized. There are a variety of reasons to consider using genome editing to change the ...yellow striate mosaic virus (Gao et al., 2019) or sonchus yellow net rhabdovirus (Ma et al., 2020) have already...

  13. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...RNAs." PloS one 11.2 (2016): e0149483. Rogers, Jameson K., and George M. Church. "Genetically encoded ...-tagged human ORF clones to allow testing and comparison of different cell-free expression systems; the...

  15. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...’s software in order to generate signals in a reasonable range. If colleagues in your group already have...interest can still be gated out later. Based on personal preference, some people like to make stricter ...

  16. CRISPR 101: Homology Directed Repair

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    Blog Post
    ...Site-Specific Genome Alterations. Edited by Doudna J and Sontheimer EJ. Methods in Enzymology Vol. 546; (2015). PubMed...inactive CRISPR-Cas9 using asymmetric donor DNA. Richardson CD, et al. Nat Biotech. 34(3); (2016). PubMed...

  17. How to Negotiate a Successful Lab Start Up Budget

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    Blog Post
    ...structure associated to these tools, skills, and personnel Define pricing according to all of the needed ...managers, and techs’ financial books.  To find personnel salary ranges, go to online job information sites...

  19. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...fluor through a process known as bioluminescent resonance energy transfer (BRET). The fluor then emits light...calcium release have been made using fluorescent resonance energy transfer (FRET) probes. These probes generally...

  20. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ... J. H., Kim, Y. S., Machado, T. A., Quirin, S., Benson, B., Kadmon, J., Raja, C., Chibukhchyan, A., Ramakrishnan... Jason Nasse...
Showing: 441 - 460 of 718 results