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Showing: 441 - 460 of 759 results

  1. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...The shredder What sets Cas3 apart from Cas9 and almost all other Cas enzymes is its method of DNA cleavage...abilities   Choosing a Cas While Cas9 may be the most adaptable Cas currently available, the other Cas...

  2. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...sharing your science - which can drive away even the most enthusiastic of learners.  What contributes to cognitive...audience to take on, just to understand you at the most basic of levels.  Concepts This can be obvious, ...

  3. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ... a number of research and clinical applications. Most of the time, when someone speaks about an affinity...thing to go when downsizing an affinity reagent, most of the alternative reagents have reduced immunogenicity...

  4. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...was successfully used in conditions that are the most similar to yours. Take a look at the publications... You now know that application validation is the most important factor in antibody selection, but what...

  5. Antibodies 101: Polyclonal Antibodies

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    Blog Post
    ... is further processed to isolate the antibodies. Most commonly, antibodies of the IgG isotype are used...When to stay away from polyclonal antibodies The most prevalent issue with polyclonal antibodies is their...

  6. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...dry lab scientists (or vice versa), then you have almost certainly experienced some of the hurdles I needed...needed to overcome.  I eventually pinpointed and (mostly) solved two main problems with Addgene’s cross ...

  7. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...relationships (also known as “networking”), one of the most common questions I receive is “Do I have to be on...to you (unless they’ve chosen to hide it). While most people use LinkedIn messaging, not everyone likes...

  8. Gendered Innovations: Why Does Sex of the Cell Matter?

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    Blog Post
    ... 145,000 more quality-adjusted life years. While most of the results were positive, the analysis did find...et al. provide a table indicating the sex of the most commonly used cell lines appearing in American Journal...

  11. Design Tips for Prime Editing

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    Blog Post
    ...Primer binding sites with 40–60% G/C content are most likely to be successful, although sequences with...which helps users select pegRNA designs that are most likely to drive efficient editing. This is not the...

  12. Hot Plasmids February 2024

    Type
    Blog Post
    ...in young macaques and vascular bias in mature marmosets. CAP-Mac also robustly expressed fluorescent reporters...(amino acid sequence DYKDDDDK) may be one of the most versatile. Whether you plan to purify or image a...

  13. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    .... As the depositing scientist, you will have the most involvement with Step 1, and, while data entry can...every so often and this ensures you are using our most up-to-date version! We hope the deposit spreadsheet...

  15. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...similar homology to the site of interest or create mosaic animals where not all cells in the blastocyst were...small molecule that regulates Cre expression. The most common drug used to induce the expression of Cre...

  16. Hot Plasmids - October 2020

    Type
    Blog Post
    ...traditionally introduced in Caenorhabditis elegans on extrachromosomal arrays can lead to artifacts due to overexpression...flow cytometry. Cells grown in high IL-3 showed mostly blue fluorescence, indicating a quick cell cycle...
Showing: 441 - 460 of 759 results