...particles, the functional infection unit. Each particle has a viral envelope, which fuses with the target cell...the fear of random genomic integration causing mutagenesis in the host. Adenoviruses can deliver relatively...non-integrating and infect non-dividing cells. AAVs primary advantage over adenovirus is their low immunogenicity, which...Dividing Cells Moderate Immunogenicity Insertional Mutagenesis Possible Lentivirus Infects non-dividing...non-dividing cells Insertional Mutagenesis Possible Adenovirus Remains Episomal (Little Risk of...of Insertional Mutagenesis) High Gene Expression Infects Non-Dividing Cells Large Packaging Capacity (... AAV Remains Episomal (Little Risk of Mutagenesis) Very Low Immunogenicity Infects Non-Dividing...

...applicable in DSB-induced genome engineering, it has biological importance for the repair of breaks that...directed repair and genome engineering CRISPR-Cas9 has revolutionized the genome engineering world and made... chromosomal aberrations. Pfeiffer P, et al. Mutagenesis (2000). PubMed. Multiple Pathways of Recombination...

...bridge helix. Created with BioRender.com.   Cas9 has two major lobes, called NUC (for nuclease) and REC...gray). Target DNA strand, light gray.   Once it has found a PAM site, Cas9 needs to check for complementarity...orange) and HNH (red) domains, while the REC lobe has been hidden for visibility. A RuvC loop caps the ... and different techniques all bring their own advantages—and blind spots—to these questions. Each new ...

...emerging validation technology. Digital PCR has several advantages over mismatch nuclease assays that will...As mentioned, these assays have several major advantages over the commonly employed mismatch nuclease ...bi-allelic mutations: Perhaps the most noteworthy advantage of digital PCR assays is that they are easily .... 6. Fu, Y. et al. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells...

...senior mentors available to go around. My advice has been to find 5 interested peers and form your own...step-by-step, how-to guide to get one started. Advantages of the group mentoring format Adding peer advice...Because the participants are at a similar development stage, they have a lot to learn from each other. Some ...the group. The group can be one gender and career stage (e.g. all women, all men, all postdocs, all grad...support each other as you transition to the next stage in your careers! Stay in touch…this group will be...

...quantified at 595 nm. This method is time consuming and has low sensitivity so it’s not used much. However, the...concentration. It’s important to consider the advantages and disadvantages of each method and when one method might...

...the most widely used CRISPR nuclease, recent work has shown that alternative enzymes may better suit specific...Joung and Jennifer Doudna Labs used targeted mutagenesis to generate hyper accurate Cas9 (HypaCas9). This... sites. Using a combined random and directed mutagenesis approach, the Anna Cereseto Lab created pX-evoCas9...experimental approaches, knowledge of both the advantages and limitations of each Cas enzyme can help determine...

...since its development, CRISPR/Cas9 genome editing has been used to study the effect of gene knockout in...-target effects and/or toxicity, it would be advantageous to instead use a preexisting, conserved cellular...leading nucleotide could be processed, a distinct advantage over other methods that favor certain nucleotides...potential of PTGs. Applications of PTGs The major advantage of the PTG system is its universality; robust ...

...Collin’s Lab in 2009. From 2009 to today, Sample6 has changed focus and names, swapped leadership, and ...of technologies, effectively communicating the advantages embodied, and driving the enterprise value of...plan competition and advance investors to the next stage of diligence." In business competitions everything... be OK with the constant fear of failure at all stages in the startup". To gain insight into "the random-walk...

...?” Some of the Tweeted comments: “A good mentor has your goals/needs/situation in mind, rather than (...being coddling, to listen without being taken advantage of, and to respect my students as developing colleagues...

...PAM motif, averaging 19 targets per gene. The lab has previously used the library with SpdCas9 to perform...lab discovered a new Cas protein, CasX, from metagenomic analysis of microbial DNA from groundwater. Now...

...with chemicals in it,” leaving me to wonder how he has survived this long without downing any dihydrogen...graduate with my BSc in Anatomy, the University of Otago had established the Centre for Science communication...Department of Science Communication at the University of Otago. Her research focuses on misinformation about sexual...

...AAV for your experiment. The Addgene viral service has now expanded to offer many of our control plasmids...tools for light mediated neuronal control. The advantages of optogenetics lie in the spatiotemporal control...recombination of lox sites. This system takes advantage of the extensive supply of transgenic reporter...

...titers found in our in-stock preps. If a request has technical considerations that may affect expected...specialist will reach out to you during the request stage, before you order.  Timeline Once your order is ...

...to a variety of careers outside of academia. She has had previous graduate students and postdocs go on...interviewed Addgene depositors who are at various stages in their careers to get advice on choosing a lab...

...amplification are endA negative, meaning the gene has been removed/mutated to neutralize the nuclease activity...elution of plasmid DNA through their kit. The advantage of using a Tris buffer is that it allows better...

...logical pattern across the poster. This style has the advantage of being different and potentially more catchy...to your audience. With a poster, you have the advantage of being able to verbally communicate your work...and acknowledgements sections are inserted. The advantage of this style is that it will display your data...

...nucleases to generate an engineered mouse model has advantages over the injection of a targeting vector alone... to create engineered mouse models at embryonic stages, and the pros and cons of different genome editing...mice are engineered during the embryonic stem cell stage using one of several techniques. The longest standing...

...platform. During the conference If the conference has a social media policy, this should be on their website...conference hashtag to set one up. It’s also helpful to write your Twitter handle on your nametag for the ...Check out the conference website for any relevant hashtags and handles to help you find other scientists ... share their research on social media. Use the hashtag if you are live-tweeting any sessions that the ...

...Addgene. The popular CRISPR/Cas9 genome editing system has been modified for use with AAV; this system represents...expression in non-dividing cells and is a key advantage of rAAV. AAV Serotypes Eleven serotypes of AAV...