Addgene

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Showing: 421 - 440 of 581 results

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Building and Validating Recombinant Antibodies: A Case Study

Type

Blog Post

...detect multiple targets with different mAbs in the same experiment. The Trimmer Lab found that they could...
GCE4All: Making Genetic Code Expansion Accessible

Type

Blog Post

...naturally, rarely are the optimization parameters the same for all three components, meaning that the final...
Viral Vectors 101: Types of viruses

Type

Blog Post

...low immunogenicity, meaning it does not induce the same high immune response as retroviruses. This virus...
Viral Vectors 101: Viruses as Biological Tools

Type

Blog Post

...designed to make you sick…well, you feel it. But the same features that make these familiar viruses such a...
Sharing is Caring: But How to Distribute Open Hardware?

Type

Blog Post

...very difficult to adapt the device to one's own samples and limits the use of microscopy in open science...
Dos & Don'ts When Publishing a Scientific Manuscript

Type

Blog Post

... journals or as many journals as possible at the same time. Take your time picking the appropriate journal...
Plasmids 101: Yeast Vectors

Type

Blog Post

...modify proteins yet they still share many of the same technical advantages that come with working with...
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

Type

Blog Post

... practice to compare degradation rates between samples treated with or without an autophagy inhibitor....
Plasmids for Endogenous Gene Tagging in Human Cells

Type

Blog Post

... For an N-terminally tagged protein, we used the same strategy, inserting the tag and the linker preceding...
CRISPRainbow and Genome Visualization

Type

Blog Post

...plasmids from this publication at Addgene. 4. Kiani, Samira, et al. “Cas9 gRNA engineering for genome editing...
Truncated gRNAs for Regulating Gene Expression

Type

Blog Post

...2015): 823-826. PubMed PMID: 26167643. 3. Kiani, Samira, et al. "Cas9 gRNA engineering for genome editing...
Visualizing Protein Turnover In Situ

Type

Blog Post

... suspected to interact, are added to a cellular sample. Both antibodies have a short antibody specific...
Human Germline Editing Using CRISPR

Type

Blog Post

...deletion in MYBPC3 donated skin, blood and semen samples. Ma et al. designed plasmids containing Cas9 and...
Celebrating 15 Years of Scientific Sharing

Type

Blog Post

...community is loving it. Almost 10,000 viral vector samples were distributed in 2018 and we are poised for ...
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

Type

Blog Post

...other hand, using a constitutive promoter in the same background resulted in a 20-fold variation of gene...
Antibodies 101: Conjugation

Type

Blog Post

... antibody will have been verified to perform the same as its unconjugated version, but if you find yourself...
Troubleshooting Your Plasmid Cloning Experiment

Type

Blog Post

...Remember to quantify the DNA concentration of all the samples by gel or by nanodrop to assemble the fragments...
Summer SciComm Series: Cognitive Load

Type

Blog Post

... way I’ve come up is this: It takes me about the same amount of time to read a 100-page genre fiction ...
Career Coaching for Scientists: Why and Where Do I Find One?

Type

Blog Post

...Consulting frequently do sessions by Skype or phone. Samantha Sutton is another very experienced and successful...
Making CRISPR Plasmids Using Fragmid

Type

Blog Post

...(GG) cloning approach. Individual modules and a sample ligation are depicted. (Bottom) Schematic depicting...

Showing: 421 - 440 of 581 results

Results per page:

Building and Validating Recombinant Antibodies: A Case Study

GCE4All: Making Genetic Code Expansion Accessible

Viral Vectors 101: Types of viruses

Viral Vectors 101: Viruses as Biological Tools

Sharing is Caring: But How to Distribute Open Hardware?

Dos & Don'ts When Publishing a Scientific Manuscript

Plasmids 101: Yeast Vectors

Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

Plasmids for Endogenous Gene Tagging in Human Cells

CRISPRainbow and Genome Visualization

Truncated gRNAs for Regulating Gene Expression

Visualizing Protein Turnover In Situ

Human Germline Editing Using CRISPR

Celebrating 15 Years of Scientific Sharing

Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

Antibodies 101: Conjugation

Troubleshooting Your Plasmid Cloning Experiment

Summer SciComm Series: Cognitive Load

Career Coaching for Scientists: Why and Where Do I Find One?

Making CRISPR Plasmids Using Fragmid

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