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...evolution to create APEX2. They have now applied the same directed evolution technique to the biotin ligase...

...resistance gene followed by the target site (the same sequence you’d like to mutate in the genome) and...

...detect multiple targets with different mAbs in the same experiment. The Trimmer Lab found that they could...

...Bioluminescence Resonance Energy Transfer (BRET) works the same way, except that energy is transferred from a luminescence...

...While both lentiviruses and γ-retroviruses use the same gene products for packaging (Gag, Pol, and Env),...

...monitoring of different factors, in different or the same cellular location, all at once! Small molecules ...

...naturally, rarely are the optimization parameters the same for all three components, meaning that the final...

...low immunogenicity, meaning it does not induce the same high immune response as retroviruses. This virus...

...designed to make you sick…well, you feel it. But the same features that make these familiar viruses such a...

...very difficult to adapt the device to one's own samples and limits the use of microscopy in open science...

...characterized and the system allows you to add this same antibody to any protein to get really beautiful ...

... journals or as many journals as possible at the same time. Take your time picking the appropriate journal...

...modify proteins yet they still share many of the same technical advantages that come with working with...

...other hand, using a constitutive promoter in the same background resulted in a 20-fold variation of gene...

... suspected to interact, are added to a cellular sample. Both antibodies have a short antibody specific...

...deletion in MYBPC3 donated skin, blood and semen samples. Ma et al. designed plasmids containing Cas9 and...

... practice to compare degradation rates between samples treated with or without an autophagy inhibitor....

...plasmids from this publication at Addgene. 4. Kiani, Samira, et al. “Cas9 gRNA engineering for genome editing...

...Remember to quantify the DNA concentration of all the samples by gel or by nanodrop to assemble the  fragments...

...community is loving it. Almost 10,000 viral vector samples were distributed in 2018 and we are poised for ...