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  2. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    ...Citing Article" - such as cloning their gene of interest into a previously deposited vector - then you'...locate other papers that have used your plasmid of interest, which may help you identify relevant protocols...

  3. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    ...trainees at all. For grad students and postdocs interested in taking matters into their own hands, there...I am happy to work with any group of trainees interested in designing career exploration programs or peer...

  5. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ...this plasmid has a U6 promoter followed by BbsI restriction sites into which you can clone your 20 nucleotide...can use to express both SpCas9 and your gRNA of interest in a variety of mammalian cell lines. Alternative...

  6. Fluorescent Proteins 101: History of Fluorescent Proteins

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    Blog Post
    ...to be used as research tools is particularly interesting. Although humans have known about bioluminescent...deciding which one is right for you, so if you’re interested in learning more about fluorescent proteins, ...

  8. Let There Be LITE Plasmids

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    Blog Post
    ...can be applied non-invasively, its use is not restricted to cultured cells but extends to freely moving...plasmids have clear application to anyone with an interest in neuroscience, their use is by no means limited...

  9. Editor's Choice, August 2016

    Type
    Blog Post
    ...discussed further in this informative post. Interestingly, this technology may make some uses of Site ... Software Engineer Kris Shamloo wrote a very interesting post about how we develop our in house lab management...

  10. Modulate the Activity of 17 Signaling Pathways with One Kit!

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    Blog Post
    ...screen for pathways that confer any phenotype of interest, not just drug resistance,” he said. “It could...contacted by investigators all over the world who are interested to use our libraries to do things - sometimes...

  11. Some Like it Hot: Thermostable GeoCas9

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    Blog Post
    ...discovered? The discovery of GeoCas9 started with an interesting question: why engineer a new Cas9 when you can...CRISPR Methods for Bacterial Genome Engineering   Interested in using CRISPR/Cas9 RNPs? Check out this post...

  13. What's New in CRISPR - September 2019

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    Blog Post
    ...double-stranded DNA break at the genomic loci of interest and on either side of the cargo (ex: fluorescent...the double-stranded break in the genomic loci of interest. As the universal sgRNA is not predicted to target...

  14. Addgene is Expanding Our Viral Vector Service!

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    Blog Post
    ...subset of plasmids will have technical or legal restrictions that make them ineligible for use in this service...consideration will have a banner on their material page. Interested scientists can expand the banner to select their...

  16. Understanding GPCR Signaling Using TRUPATH

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    Blog Post
    ...activated in response to a ligand binding a GPCR of interest. How does TRUPATH work? The TRUPATH collection...transfected with plasmids expressing the GPCR of interest, a Gα-Rluc8 plasmid, and the appropriate Gγ-GFP2...

  17. Fostering communication in remote collaborations

    Type
    Blog Post
    ...between recurring meetings, you should share interesting results or ask questions to your collaborators...microscopes and allowed me to investigate my protein of interest in a French clinical cohort, neither of which ...

  20. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...added to the N or C terminus of your protein of interest. Many of these tags also have commercially available...makes it amenable to being left on your protein of interest, especially if downstream antibody detection is...
Showing: 421 - 440 of 748 results