...Kamens several years ago through the Healthcare Businesswomen’s Association and it was through this connection... systematically test the function of all 20,000 genes in disease models and then focus drug discovery ...early age, I was fascinated by the impact that medicines can have on human health. As a teenager in high...the field of gene therapy.” He’s also worked on vaccines throughout that time. Now Vandenberghe is an Associate...

...issues by creating immortal antibody-producing cell lines (hybridomas) that secrete antibodies in culture ...production. However, even clonal hybridoma cell lines can produce more than one mAb (Bradbury et al., ...hybridomas can lose gene expression of the mAb-encoding genes, die, or fail to revive after cryopreservation (...Wilkinson IC, Tandon AK, Anderson S, Bladen CL, Jones B, Aldred SF, Bestagno M, Burrone O, Maynard J, ...

...capable of robust expression of high G+C content genes, as validated through monitoring the expression ...adapted for RNAi experiments for mammalian cell lines. The Turner Lab Human messenger-RBP sgRNA Library...MYC-ChIP-seq data from several MYC-dependent cancer cell lines. It can be applied for identification of essential...pooled library from Junjie Chen's lab targets human genes known or suspected to be involved in DNA damage ...

... Addgene to gene-edit mutations into XP genes and other genes involved in photosensitivity in melanocytes...hemiplegia) that are often painful and accompanied by migraines. Those attacks can be brought on by a number of...plan to use CRISPR-Cas9 technology to create cell lines and/or mouse models carrying different SOX10 mutations...

... cell type of interest better than immortalized lines derived from the same cell type; however, their ...interest into primary cells, so most primary cell lines require viral infection. A new paper from Niels ...alter protein function or localization. Thus, the usefulness of CPPs must be evaluated on a case-by-case basis...biallelic mutations. Importantly, hESCs retained their stemness, indicating that iTOP may be valuable for CRISPR...

... delete/overexpress endogenous genes or to introduce heterologous genes, but the trend of research has...mimic natural transcriptional programs on multiple genes. Modifying the CRISPRi system for multidirectional...programs to differentially alter the levels of five genes that encode biosynthetic enzymes for violacein, ...

...screens, enabling genome-wide interrogation of the genes required for cellular processes. For a successful...that constitutively express Cas9 and screen for genes that affect Th2 differentiation and activation, ...-LPs and accelerate the discovery of additional genes and small-molecule probes to aid in the understanding...to different lipid compositions of C. elegans membranes, which might beneficially influence the rhodopsin...

...keeping the system to minimal size. To do so, they harnessed the tRNA processing mechanism, which is conserved...lentivirus, demonstrating simultaneous editing of genes against multiple genetic diseases, such as muscular...for correcting mutations or installing functional genes to study and potentially treat polygenic diseases... that would perturb the unprecedented number of genes with a single viral vector.   Fig. 1: Schematic...

...medical conditions had been swapped with made up illnesses. They were then asked to evaluate the quality ...methodology makes it easier to spot even minor weakness or limitations in a piece of research (Drummond... mask designs could work to slow the spread of illness. I later helped run workshops where I showed people... jargon that even scientists in neighbouring disciplines might struggle to understand. Why should we trust...

...assignment to discuss goals, expected outcomes, timelines and deadlines. Agree on a way to review project progress...identify constraints and boundaries. Where are the lines of authority, responsibility and accountability?...try "Why Aren't You Delegating" in the Harvard Business Review. Another excellent blog on delegation tips...

...containing plasmids differing in their resistance genes, as one does not need to prepare separate batches...

...learn new tasks and beat humans at the ancient Chinese game Go). In addition, there was an entire story-telling...neuroscientists are using techniques from across different disciplines to better understand neurobiology and human cognition... provide a highly versatile means of delivering genes to many different cell types with minimal immunological...effects. AAVs are used in experiments targeting genes to specific cell types in the brain, mapping neuronal...

...and funders in the Transparency and Openness Promotion Guidelines; preregistration to increase clarity...variable actually makes a big difference for the robustness of the findings.   How do we make research more...code, and other materials. The use of reporting guidelines can also help remind us to include all of the...

...frequently used Cas9, spCas9 from Streptococcus pyogenes, this sequence is 3’-NGG. But Cas9s found in other...PAM sequence that the Cas nuclease recognizes determines site accessibility. Cas9s with a broader PAM ...Joung and Jennifer Doudna Labs used targeted mutagenesis to generate hyper accurate Cas9 (HypaCas9). This... sites. Using a combined random and directed mutagenesis approach, the Anna Cereseto Lab created pX-evoCas9...

...cloning uses short sticky ends to insert genes into plasmid backbones, base pairing between these ~4 base ... different types of PCR-generated inserts. By harnessing the power of DNA repair, you can assemble multiple...2024.  References and Resources Further reading Harnessing homologous recombination in vitro to generate...

...observe a phenotype, the modification of multiple genes is often necessary. Guide RNAs (gRNAs) are commonly... al constructed polycistronic glycine tRNA-gRNA genes (PTGs) in the forms tRNA-gRNA and tRNA-gRNA-tRNA...constructed PTGs containing 8 gRNAs targeting 4 MAPK genes, again using rice protoplasts. Each pair of gRNAs...knockout, or multiple gRNAs may be used to target genes with overlapping activity. Multiple gRNAs can also...

... help you verify the ends of the insert. Longer genes may require internal primers that anneal to a specific...

...digests needed in molecular biology, and thaw cell lines. There are many different types of water baths that...

...ion-pump encoding genes HCN1 KCNJ2 KCNJ13 GABRG1 KCNIP2 KCNQ1 Examples of some genes encoding gap junction...fluorescent protein. This GEVI features increased brightness and improved plasma membrane specificity. Stable...encoded, light-gated ion translocators to control tumorigenesis." Oncotarget 7.15 (2016): 19575. PubMed PMID...

...systems including the popular S. pyogenes Cas9 (Rauch et al., 2017, Hynes et al., 2017). However, Acr inhibition...What’s great about CASANOVA? It works with the S. pyogenes Cas9, dCas9-effector fusions, and xCas9, and requires...compatible approach to simultaneously clone two genes-of-interest (GOI) into two independent expression...